1.Quantative measurement of tumor-associated antigen by autoradiography.
June Key CHUNG ; Dong Soo LEE ; Myung Chul LEE ; Chang Soon KOH ; James C REYNOLDS
Journal of the Korean Cancer Association 1991;23(4):740-748
No abstract available.
Autoradiography*
2.Effects of UV Light on DNA Synthesis Studied by Autoradiography.
Jai Il YOUN ; Jun Woo SHIN ; Yoo Shin LEE
Annals of Dermatology 1989;1(1):6-9
No abstract available.
Autoradiography*
;
DNA*
;
Ultraviolet Rays*
3.The effects of UVB and UVA on the scheduled and unscheduled DNA synthesis of mouse epidermal cells.
Kyu Han KIM ; Bang Soon KIM ; Sang Eun MOON ; Jai Il YOUN
Korean Journal of Dermatology 1992;30(2):186-196
After UV irradiation to mouse epidermis, autoradiography using H-thymidine was performed to study the effects of UV on scheduled and unschcdu ed DNA syntheses. The results can be summarized as follows. l. In case of UVB 100mJ/cm, heavily labeled cells (HLCs) representing scheduled DNA synthesis began to decrease immediately after UVB irradiai,ior and were significantly decreased after 24 hours, but then recovered after 48 hours. The ecovery was maintained from 72 hours, up to 7 days. Sparsely labeled cells (SLCs) repres inting unscheduled DNA synthesis were significantly increased from immediately after UV 3 irradiation and up to 6 hours later. Repair was present at 24 hours, and was maintained at 48 hours, 72 hours, and 7 day after irradiation. 2. In case of UVA, UVA 10J/cm did not affect DNA synthesis sign ficantly. But in cases of UVA 30J/cm and 50J/cm, HLCs began to decrease immediately after UVA irradiation and were significantly decreased for 24 hours. Recovery occured at 48 hours, and was maintained from 72 hours up to 7 days. SLCs were significently increased immediately after and up to 6 hours after UVB irradiation. Repair generally occurred after 24 hours, and was maintained at 48 hours, 72 hours, and 7 days after ir rad ation.
Animals
;
Autoradiography
;
DNA*
;
Epidermis
;
Mice*
4.Distribution of the muscarinic receptors and characterization in the brain of wistar rats and spontaneously hypertensive rats(SHR strain) by digital autoradiography.
In SOHN ; Myung Chul LEE ; Chang Soon KOH
Korean Journal of Nuclear Medicine 1993;27(1):28-34
No abstract available.
Autoradiography*
;
Brain*
;
Rats, Wistar*
;
Receptors, Muscarinic*
5.Autoradiographic Investigation for 3H-Thymidine Labeling Indicies of gamma-Glutamyl Transpeptidase Positive Foci during the Rat Liver Carcinogenesis.
Kee Woon KWEON ; Hong Joo KIM ; Soong Hwan LEE ; Yeong Jung CHO ; Jong Cheol KIM ; Sung Woo KIM ; Hee Soo KIM ; In Gyu BACK ; Cheol Hun JUNG ; Yong Hyeon JO ; Chang Woo GHAM ; Dong Hoo LEE
The Korean Journal of Hepatology 1998;4(2):162-178
No abstract available.
Animals
;
Autoradiography
;
Carcinogenesis*
;
Diethylnitrosamine
;
gamma-Glutamyltransferase*
;
Liver*
;
Rats*
6.Autoradiographic Investigation for 3H-Thymidine Labeling Indicies of gamma-Glutamyl Transpeptidase Positive Foci during the Rat Liver Carcinogenesis.
Kee Woon KWEON ; Hong Joo KIM ; Soong Hwan LEE ; Yeong Jung CHO ; Jong Cheol KIM ; Sung Woo KIM ; Hee Soo KIM ; In Gyu BACK ; Cheol Hun JUNG ; Yong Hyeon JO ; Chang Woo GHAM ; Dong Hoo LEE
The Korean Journal of Hepatology 1998;4(2):162-178
No abstract available.
Animals
;
Autoradiography
;
Carcinogenesis*
;
Diethylnitrosamine
;
gamma-Glutamyltransferase*
;
Liver*
;
Rats*
7.Autoradiographic studies on the uptake and distribution of C(14)-glucose by Paragonimus westermani.
The Korean Journal of Parasitology 1971;9(1):9-16
Autoradiographic study was performed in order to know the distribution of exogenous C(14)-glucose by lung fluke, Paragonimus westermani, incubated in Tyrode medium containing 10 uCi/ml of labeled substance. After 1 hour incubation at 37C, microautoradiographs of this fluke showed that black grains derived from radioactive carbon were accumulated mainly in the parenchyme and subcuticular musculature. The muscular tissues such as oral sucker, pharynx and ventral sucker revaled considerable density of fine grains. Slight radioactivity was also observed in the regions of ovary, testes, vitelline follicles, eggs in uterus, intestinal ceca, and even in excretory bladder. Structures showing the least activity included the cuticle and uterine tubules of this fluke.
parasitology-helminth-trematoda- Paragonimus wertermani
;
autoradiography
;
biochemistry-glucose
;
Tyrode
;
glucose
8.Development of Experimental 9L Gliosarcoma Rat Brain Tumor Model.
Jong Hyun KIM ; Seung Hoon LEE ; Chang Hun RHE ; Sang Min YOON
Journal of Korean Neurosurgical Society 1995;24(5):497-504
Experimental brain tumor model is essential for the development of new therapeutic modalities of brain tumors and evaluation of efficacy of each therapeutic variety. Authors developed experimental rat brain tumor model with 9L and C6 cell line in Fischer rat using stereotactic method. We tried to determine the tumor occurrence rate, the ideal time for secondary experiment using this brain tumor model, and the duration between the onset of neurological signs and the time of expiration. We performed autoradiography for each cell line to evaluate the reliance of the tumor model. We could make good tumor model in all the cases of experiment and except to use it in another extension of experiment.
Animals
;
Autoradiography
;
Brain Neoplasms*
;
Brain*
;
Cell Line
;
Gliosarcoma*
;
Rats*
9.Ontogeny of Adrenergic Receptors in the Rat Amygdala.
Yoon Young CHUNG ; Choon Sang BAE
Korean Journal of Anatomy 1998;31(3):447-463
The mammalian amygdala comprises a heterogeneous complex of cytoarchitectonically, histochemically and connectionally distinct nuclei. To investigate the developmental changes and regional distributions of adrenoceptor binding sites in the adult and postnatal rat amygdala (postnatal days 0, 5, 10, 15, 20, 30), in vitro autoradiography was performed. Binding sites for the alpha1-adrenoceptor ligand [3H]-prazosin, the alpha2-adrenoceptor ligand [3H]-rauwolscine, and the beta-adrenoceptor ligand [125I]-iodocyanopindolol were visualized by the in vitro autoradiography, and anatomically localized by comparing the autoradiograms to Nissl- and acetylcholinesterase-stained sections. On toluidine blue- and acetylcholinesterase-stained sections of the amygdaloid complex of the rat, three major divisions can be distinguished: the cortical-like nuclear group, medial nuclear group, and central nuclear group. The basolateral nuclear group of the cortical-like nuclear group was divided into three subregions, the basal, the basolateral and the basomedial amygdaloid nucleus. Between the medial and the basolateral amygdaloid nucleus, the intercalated nuclei were observed. The highest number of alpha1-adrenoceptor binding sites was detected in the central and the lateral amygdaloid nucleus. The nuclei most strongly labeled by [3H]-rauwolscine were those in the medial part of the amygdaloid complex. The pattern of the beta-adrenoceptor binding was relatively diffuse, the medial amygdaloid nucleus was most strongly labeled among the amygdaloid nuclei. At the postnatal day 0, adrenergic receptor binding sites were only weakly labeled. The expression of a1-adrenoceptor binding was rapidly increased in the central amygdaloid nucleus at the postnatal day 5, and between the postnatal day 10 and 15, the concentration of bindig sites reached the adult levels. The expression of alpha2- and beta-adrenoceptor binding was increased in most amygdaloid nuclei at the postnatal day 10, and higher density was observed at the postnatal day 30. In the adult, the expression of adrenoceptor binding was relatively low in most nuclei when compared to postnatal day 30. These findings may provide evidence that alpha1-adrenoceptor is involved in regulating amygdaloid development and function more specifically than alpha2- and beta-adrenoceptor during postnatal development. These results indicate that the regional distributions of alpha1-, alpha2-, and beta-adrenoceptor show some differences from those of the other mammalian species reported.
Adult
;
Amygdala*
;
Animals
;
Autoradiography
;
Binding Sites
;
Humans
;
Rats*
;
Receptors, Adrenergic*
10.Metabolism of C(14)-glucose by Paramphistomum cervi.
Byong Seol SEO ; Han Jong RIM ; Sang Il LEE ; Dae Kwan PARK ; Sang Chan MOON
The Korean Journal of Parasitology 1965;3(1):5-9
The trematode Paramphistomum cervi empolyed in this experiment was obtained from the reticulum of cattle slaughtered at the local abbatoir. The worms were selected and washed several times in normal sterilized saline solution. Each about ten of intact worms were incubated in 50 cc volume of special incubation flasks with incubation mixture consisting of 50 cc of Krebs-Ringer phosohate buffer (pH 7.4) to which were added universally labeled C(14)-glucose and non-radioactive carrier glucose concentration of 200 mg per cent. The worms were allowed to incubate for 3 hours in the incubator at 38 C. After incubation period, respiratory CO(2) samples from central wall of incubation flask were analysed for total CO(2) production rate and their specific activity of respiratory CO(2). Glycogen samples isolated from worms were analysed for the tissue concentration and their radioactivities in order to determine the turnover rate of glycogen pool. The glucose uptake rate was determined by analysing the difference of the glucose concentration in a medium before and after incubation period. Radioactivities of these series of experiments were counted by an endwindow Geiger-Muller counter as an infinitely thin samples. The quantitative analysis of C(14)-glucose utilized by Paramphistomum cervi was summerized as the following. The glucose uptake rate by Paramphistomum was a mean value of 2.32+/-0.27 micro-mole/hr/g of wet wt. and total CO(2) production rate by the worms averaged 10.85+/-0.41 micro-mole/hr/g of wet wt. The relative specific activities of respiratory CO(2) averaged 49.72+/-13.20 per cent. Thus, a mean of 49.72 per cent of total CO(2) production rate was originated from the glucose in the medium, therefore the rate of CO(2) production derived from medium glucose was mean of 5.24+/-2.16 micro-mole/hr/g of wet wt. Thus, the average value of 37.46+/-5.28 per cent of glucose utilized by the worms from the medium glucose was oxidized to respiratory CO(2). The tissue concentration of Paraphismum was a mean of 41.56+/-5.82 micro-mole/hr/g of wet wt or 4.16+/-0.72 per cent/g , and the turnover rate of glycogen pool yielded with a mean of 0.12+/-0.014 percent/hr or 0.06+/-0.04 mg/hr/g of wet wt. Therefore, a mean value of 16.75+/-4.84 per cent of glucose was incorporated to the glycogen. These data account for that at least 54.21 per cent of the utilized glucose by the worms participated in furnishing the oxidation into respiratory CO(2) and the synthetic process into glycogen. According to the above data of the experiment, it is suggested in the metabolic process of glucose by the Paramphistomum that the synthetic process into the glycogen is less active than the oxidative process into the resppiratory CO(2).
parasitology-helminth-trematoda
;
Paramphistomum cervi
;
autoradiography
;
biochemistry
;
glucose
;
metabolism
;
CO(2)
;
glycogen