Objective To investigate the levels of serum tHcy and Lp(a)and their association with insulin resistance in patients with type 2 diabetes and CHD. Methods T2DM patients were divided into two groups with CHD and without CHD in addition to normal control.The fasting serum levels of tHcy,Lp(a),FPG and FIns were detected, ISI were calculated，and the correlations among them were analyzed. Results The serum levels of tHcy,Lp(a),FIns,FPG of T2DM plus CHD were significantly higher than those of T2DM without CHD and controls(P<0.01~0.05).ISI was significantly lower in T2DM cases with CHD than those of T2DM cases without CHD and controls(P<0.01). ISI was negatively correlated with tHcy and Lp(a)in T2DM plus CHD group(P<0.05)but not correlated with those in T2DM case without CHD group(P>0.05). Conclusions Detections of the serum levels of tHcy and Lp(a)of T2DM play significant roles in knowing patient condition,guiding clinical treatment and earlier preventing and treating vascular complication of diabetes.

Retinopathy is a major cause of morbidity in diabetes and remains the primary cause of new blindness. Therefore, it is necessary to find new drug to treat diabetic retinopathy. Type 2 diabetes mellitus (T2DM) rats were induced by injection (ip) with streptozotocin (STZ) 35 mg·kg-1 and fed with a high-carbohydrate/high-fat diet 2 weeks later. From week 17 to 32, diabetic rats were given different doses of berberine 75, 150, and 300 mg·kg-1, fenofibrate 100 mg·kg-1 and rosiglitazone 4 mg·kg-1, separately. Retinal structure was observed with hematoxylin-eosin staining and peroxisome proliferator-activated receptors (PPARs) α/δ/γ protein expressions were detected by immunohistochemistry. The retina of control rats was thicker than that of other groups, 16 weeks treatment with berberine (150 and 300 mg·kg-1) and rosiglitazone 4 mg·kg-1 thickened the diabetic retina, but no difference existed in retinal structure among groups. Both berberine (150 and 300 mg·kg-1) and rosiglitazone 4 mg·kg-1 significantly decreased PPARγ expression in diabetic retina;while berberine (150 and 300 mg·kg-1) and fenofibrate 100 mg·kg-1 obviously increased both PPARα and PPARδ expressions in diabetic retina. Berberine modulates PPARα/δ/γ protein levels in diabetic retina which may contribute to ameliorate retinopathy complication induced by STZ and a high-carbohydrate/high-fat diet. It is expected that berberine might be a more beneficial drug to treat diabetic retinal complication comparing with fenofibrate and rosiglitazone.

10.3969/j.issn.1000-3606.2013.06.001

Objective To observe the therapeutic effect of electroacupuncture plus laser intravenous irradiation therapy on dysfunction caused by craniocerebral injury. Methods One hundred and seventy cases of craniocerebral injury were randomly and equally divided into a treatment group and a control group. The control group was managed with routine treatment, while the treatment group with the electroacupuncture plus the laser intravenous irradiation on the basis of the routine treatment. Results After the treatment, there were significant differences between the two groups in terms of Glasgow Outcome Scale(GOS), Dyscinesia Scale(DS) and Karnofsky Performance Scale(KPS) evaluations. Conclusion The therapeutic effect and clinical outcome of electroacupuncture plus intravenous laser irradiation is significantly better than that in control group, indicating that electroacupuncture plus intravenous laser irradiation is a better therapeutic option for improvement of motor functions.

Congenital cataract is a common eye disease which leads to children low vision. Surgery is the main treatment. Because of children's preoperative evaluation, operation, postoperative management is more difficult than adult, the outcome of surgery is not so well. Therefore, we review the relevant congenital cataract surgery researches in recent years in order to discuss the problems and further developments in this area.

Aim To study the regulation mechanisms of deacetylase inhibitor SAHA in p21WAF1/CIP1 promoter acetylation in breast cancer MCF-7 cells.Methods We used quantitative real-time PCR, Western blot and DNA-ChIP to determine the effects on the regulation of cell cycle with SAHA treatment in MCF-7 cells.By DNA-ChIP, we assessed the acetylation level of p21WAF1/CIP1 promoter.Results SAHA significantly affected the expression of cell cycle-related factors, and induced the mRNA and protein expression of p21WAF1/CIP1.SAHA could adjust the acetylation level of p21WAF1/CIP1 promoter.Conclusion SAHA regulates the cell cycle progression by adjusting the acetylation level of p21WAF1/CIP1 promoter in MCF-7 cells.

OBJECTIVE:To explore the best way of drug application&taking in pharmacy through computer.METH-ODS:Ratio ordering method was applied to the high and low limit setups of drug stocks.RESULTS:The timely and dynamic high and low limit data setups through computer has successfully overcome the drawbacks of those from manual labor,thus more scientific and rapid drug application&taking was available.CONCLUSION:Ratio ordering method deserves to be generalization and application in drug application&taking by computer.

It is widely accepted that diabetes is intimately linked to diet. The latest researches showed that diet-microbiota interactions acted as moderators of human diabetes. Gluten-free diet and hydrolyzed protein decreased the incidence of type 1 diabetes mellitus (T1DM)by the mechanism of changes in gut microbiota and immune micro-environment. Consuming more dairy products decreased the incidence of type 2 diabetes mellitus (T2DM). High fat diet can induce insulin resistance (IR),thus increasing the incidence of T2DM. Parental dietary affected on offspring diabetes by the epigenetic mechanism. Low carbohydrate diet,dietary fiber and legumes were effective to the treatment of diabetes. Prediction of postprandial glucose level and treatment based on gut microbiota contributed to control the glucose of diabetic patients. An in-depth understanding of these mechanisms provides new ideas for the individualized and precise treatment of diabetes.

The afferent connections of the preoptic region in the rat were studied with retrograde horseradish peroxidase (HRP) method.The mico-injection syringe needle or microelectrophoretic pipette was inserted vertically into the brain. In some cases, the injection was made with the needle inclined and directed contralaterally or in inclined direction through the cerebellum.The results were as follows:Following HRP injection into the medial preoptic area, labeled neurons were found in periventricular hypothalamic gray, paraventricular nucleus, anterior hypothalamic nucleus, supraoptic nucleus, ventromedial hypothalamic nucleus, posterior hypothalamic nucleus and locus coeruleus in all cases. In some cases, labeled cells were also observed within bed nucleus of stria terminalis, dorsal raphe nucleus. In addition, in a few cases, a small number of labeled cells could be found in lateral septal nucleus, medial septal nucleus, periaqueductal gray matter and interpeduncular nucleus.After injection into the region between the medial and lateral preoptic area, besides the above mentioned labeled nuclei, there were still a number of labeled cells found in paratenial nucleus, nucleus reuniens and lateral hypothalamic nucleus, in some cases also found in the supramammillar nucleus, and in one case even in the cortical and medial amygdalar nuclei.Following the injection of HRP into the anterior hypothalamic nucleus, retrograde labeling of neurons was observed within the lateral septal nucleus, preoptic periventricular nucleus, medial preoptic nucleus, supraoptic nucleus, periventricular hypothalamic nucleus, paraventricular nucleus, ventromedial hypothalamic nucleus, periaqueductal gray matter and locus coeruleus in all cases. Among them, supraoptic and paraventricular nuclei were labeled bilaterally in some cases.