Objective To investigate the levels of serum tHcy and Lp(a)and their association with insulin resistance in patients with type 2 diabetes and CHD. Methods T2DM patients were divided into two groups with CHD and without CHD in addition to normal control.The fasting serum levels of tHcy,Lp(a),FPG and FIns were detected, ISI were calculated，and the correlations among them were analyzed. Results The serum levels of tHcy,Lp(a),FIns,FPG of T2DM plus CHD were significantly higher than those of T2DM without CHD and controls(P<0.01~0.05).ISI was significantly lower in T2DM cases with CHD than those of T2DM cases without CHD and controls(P<0.01). ISI was negatively correlated with tHcy and Lp(a)in T2DM plus CHD group(P<0.05)but not correlated with those in T2DM case without CHD group(P>0.05). Conclusions Detections of the serum levels of tHcy and Lp(a)of T2DM play significant roles in knowing patient condition,guiding clinical treatment and earlier preventing and treating vascular complication of diabetes.

The application of military medical equipment highly influences the effect of medical support.According to the investigation in the affiliated basic units,this paper puts forward improvement measures for the existing problems in the application of military medical equipment.

Strategic information reservation of medicinal materials is complementary to the strategic reservation of medicinal materials itself.This article discusses the advantages and disadvantages of the reservation of physical medicinal goods and mobilization of medical resources for national defense.The construction mode and operational characteristics of the information database for the strategic reservation of medicinal materials have also been discussed as well as the great significance of its widely application to the information reservation of medicinal materials.

Objective To investigate whether the early use of tetramethylpyrazine(TMP) could improve the oxygenation ,reduce the rate of pulmonary lesion ,increase the survival rate and shorten the hospital days in the patients with severe acute pancreatitis (SAP) .Methods A total of 42 patients with SAP were randomly divided into the control (C) group(21 cases ,receiving the conven-tional treatment combined with the respiratory support treatment ) .The TMP group(21 cases ,receing the conventional treatment , respiratory support and TMP treatment ) .Results In the C group ,6 cases were intubated(28 .57% ) ,9 cases were acute respiratory distress syndrome(ARDS)(42 .86% ) ,the survival rate was 80 .95% ,the average hospitalization was (21 .6 ± 7 .3)d;in the TMP group ,3 cases were intubated(14 .28% ) ,6 cases were ARDS(28 .57% ) ,the survival rate was 85 .71% ,the average hospitalization was(17 .9 ± 6 .4)d .The survival rate had no statistical difference between the two groups (P>0 .05) .In addition ,the TMP group was superior to the C group in the average hospital days .Conclusion Eerly TMP interrention significantly reduced the rate of endo-tracheal intubation and the occurrence rate of ARDS ,shorten the hospital days ,but without increasing the survival rate .

The afferent connections of the preoptic region in the rat were studied with retrograde horseradish peroxidase (HRP) method.The mico-injection syringe needle or microelectrophoretic pipette was inserted vertically into the brain. In some cases, the injection was made with the needle inclined and directed contralaterally or in inclined direction through the cerebellum.The results were as follows:Following HRP injection into the medial preoptic area, labeled neurons were found in periventricular hypothalamic gray, paraventricular nucleus, anterior hypothalamic nucleus, supraoptic nucleus, ventromedial hypothalamic nucleus, posterior hypothalamic nucleus and locus coeruleus in all cases. In some cases, labeled cells were also observed within bed nucleus of stria terminalis, dorsal raphe nucleus. In addition, in a few cases, a small number of labeled cells could be found in lateral septal nucleus, medial septal nucleus, periaqueductal gray matter and interpeduncular nucleus.After injection into the region between the medial and lateral preoptic area, besides the above mentioned labeled nuclei, there were still a number of labeled cells found in paratenial nucleus, nucleus reuniens and lateral hypothalamic nucleus, in some cases also found in the supramammillar nucleus, and in one case even in the cortical and medial amygdalar nuclei.Following the injection of HRP into the anterior hypothalamic nucleus, retrograde labeling of neurons was observed within the lateral septal nucleus, preoptic periventricular nucleus, medial preoptic nucleus, supraoptic nucleus, periventricular hypothalamic nucleus, paraventricular nucleus, ventromedial hypothalamic nucleus, periaqueductal gray matter and locus coeruleus in all cases. Among them, supraoptic and paraventricular nuclei were labeled bilaterally in some cases.

Aim To study the regulation mechanisms of deacetylase inhibitor SAHA in p21WAF1/CIP1 promoter acetylation in breast cancer MCF-7 cells.Methods We used quantitative real-time PCR, Western blot and DNA-ChIP to determine the effects on the regulation of cell cycle with SAHA treatment in MCF-7 cells.By DNA-ChIP, we assessed the acetylation level of p21WAF1/CIP1 promoter.Results SAHA significantly affected the expression of cell cycle-related factors, and induced the mRNA and protein expression of p21WAF1/CIP1.SAHA could adjust the acetylation level of p21WAF1/CIP1 promoter.Conclusion SAHA regulates the cell cycle progression by adjusting the acetylation level of p21WAF1/CIP1 promoter in MCF-7 cells.

Objective To develop developer with fast determining stopped spot of electrolyte electrode in wartime.Methods According to certain proportion configuration,the developer was prepared by using relative reagent and distilled water.Through injector to take 1ml developer and curve syringe needle,then straight insertion the type needle of electrolysis meter and pour into the developer to fill suction tube.Results The reagent can be seen various electrodes change and fast display the electrode breakdown anywhere.Conclusion The color developing reagent is low-cost,efficient and convenient to use.

Objective To explore the effect of baicalin on tumour necrosis factor-?(TNF-?) and soluble inter-cellular adhesion molecule-1(sICAM-1) of immunosuppressed rats infected with Pneumocystis carinii.Methods Forty-nine SD rats were randomly divided into 7 groups:normal control(A), immunosuppressed control(B), SMZ/TMP control(C), baicalin prevention(D), low dose(E), moderate dose(F) and high dose(G).Rats of group D received six injections at three-day intervals with 3.5 mg dexamethasone sodium phosphate for 3 weeks, while groups B, C, E, F and G received same immunosuppressor but for 6 weeks.Rats in group D were given 100 mg/kg baicalin daily for 5 weeks, and groups C, E, F and G were given 250 mg/kg SMZ+50 mg/kg TMP, low dose 100 mg/(kg?d), moderate dose 200 mg/(kg?d), and high dose 400 mg/(kg?d) baicalin daily for 2 weeks, respectively.At the end of 8th week after immunosppression, the contents of TNF-? and sICAM-1 in peripheral blood were detected by radioimmunoassay(RIA) and ELISA, respectively.The pathological change of lung was observed by lung imprint smear with gormori methenamine silver(GMS) staining and lung section with hematoxylin-eosin(HE) staining.Results The content of TNF-? in group D(2.14?0.14) ng/ml, group E(2.57?0.15) ng/ml, group F(1.46?0.14) ng/ml, group G(1.12?0.13) ng/ml andgroup C(1.59?0.14) ng/ml were higher than that of group A(0.70?0.21)ng/ml(P

It is widely accepted that diabetes is intimately linked to diet. The latest researches showed that diet-microbiota interactions acted as moderators of human diabetes. Gluten-free diet and hydrolyzed protein decreased the incidence of type 1 diabetes mellitus (T1DM)by the mechanism of changes in gut microbiota and immune micro-environment. Consuming more dairy products decreased the incidence of type 2 diabetes mellitus (T2DM). High fat diet can induce insulin resistance (IR),thus increasing the incidence of T2DM. Parental dietary affected on offspring diabetes by the epigenetic mechanism. Low carbohydrate diet,dietary fiber and legumes were effective to the treatment of diabetes. Prediction of postprandial glucose level and treatment based on gut microbiota contributed to control the glucose of diabetic patients. An in-depth understanding of these mechanisms provides new ideas for the individualized and precise treatment of diabetes.

Whether resection for colorectal cancer could achieve the same effect as open surgery had been doubted. Recently, the international large prospective random control tests and large retrospective studies have revealed that laparoscopic resection for colorectal cancer not only has short-term advantages such as minor injuries, less postoperative pain, quicker recovery of bowel function and less postoperative hospital stay, but also had at least the equivalent long-term effects in local recurrence, distant metastasis, incision implantation and long term survival. It is promising that the roll of laparoscopic surgery for colorectal cancer will be defined by more evidence-based medicine study in the near future.