Objective To provide references for clinical rational use of Chinese patent medicine. Methods Totally 387 specifications of Chinese patent medicine in our hospital were analyzed, and were judged by pregnancy contraindication according to China Pharmacopeia (2010 edition). Results The 387 Chinese patent medicines involved 32 prohibited pregnancy ingredients, 1 taboo ingredient. In 89 Chinese patent medicines contraind prohibited ingredients, 33 marked prohibited ingredients, 18 marked contraindicated ingredients, 14 marked cautious ingredients, and 24 marked without-contraindication ingredients. 68 Chinese patent medicines contained pregnancy contraindication labels but without prohibited ingredients. Conclusion A unified standard of pregnancy contraindication should be established;researches on pregnancy contraindication should be strengthened;medicine specifications should be standardized and perfected.

Objective To investigate the application value of high-sensitivity troponin T (hs-cTnT)in acute coronary syndrome (ACS).Methods Serum hs-cTnT,CK and CK-MB were detected 198 patients with chest pain including 105 cases of acute myocar-dial infarction(AMI)and 93 cases of unstable angina(UA),and 83 cases of healthy physical examination as control.Results The positive rates of hs-cTnT,CK and CK-MB in the patients with diagnosed AMI were 88.6%,48.6% and 71.4% respectively,which were significantly higher than those in the patients with UA and the healthy controls(P <0.05 ).The positive rate of hs-cTnT in early AMI patients was higher than that of CK and CK-MB.Conclusion Compared with CK and CK-MB in myocardial Zymogram, hs-cTnT has very high sensitivity and specificity for diagnosing myocardial injury,could reduce the misdiagnosis and missed diagno-sis of ACS and is a marker for diagnosing the myocardial injury in early ACS.

The gradually matured MOOC technology and its platform will promote a new round of teaching reform and innovation of information retrieval microcourse .The teaching model of information retrieval microcourse against the background of MOOC and its implementation were described with the practical teaching model of information re-trieval microcourse in Shihezi University as an example .Its problems were summarized with suggestions put forward for their solution .

Objective To study the chemical constituents of starfish Asterias rollestoni Bell.Methods The constituents were isolated by chromatography methods and identified by spectroscopic and chemical analyses.Results and Conclusion A novel compound was obtained and identified as(2S,3S,4R)-2-(13'-methyl-pentadecanoylamino)-nondecane-1,3,4-triol.The compound was reported first time and named as rollceramide A.

OBJECTIVE To estimate the current situation about nosocomial infections(NI),and find possible reasons to improve measures.METHODS A point-prevalence survey of NI in all inpatients was made on June 11,2007.RESULTS Of the 755 patients investigated,the prevalence rate of NI was 4.77%(36 cases).In all of the NI occurred,the common sites of NI were respiratory tract(69.30%),superficial surgical incision(13.80%),and skin soft tissue(8.33%).The usage rate of antibiotics was 57.40%,The single usage rate of antibiotics was 60.70%,double antibiotics usage 38.30%,and triple antibiotics usage 0.90%.CONCLUSIONS The departments of nephrology and neurosurgery are with high NI rate.Strengthening the management of severe patient and rational using antibiotics can help to reduce prevalence rate of NI.

Objective To investigate the influence of inhalation anesthestic desfluran (Des) on the learning and memory abilities of rats and the protective role of chrysophanol.Methods Totally,50 male and 50 female rats,aging 24 months and weighing (500 ± 10) g,were randomly divided into five groups:normal control group,Des group,low-,mediumand high-dose Chr group (0.1,1.0 and 10.0 mg·kg-1),with 20 rats in each group.After anesthetization for 24 h,the Morris water maze was used to investigate the abilities of learning and memory of rats.The amount of Aβ1-42 was determined by ELISA assay,and the apoptosis of rat hippocampal neurons in five group was observed by TUNEL assay.Furthermore,the expression levels of Bcl-2,Bax and Caspase-3 were examined by Western blotting.The activity of acetylcholinesterase in each rats hippocampus was determined using iron trichloride chromogenic spectrophotometer colorimetric analysis method.Results Compared with the normal control group,the mean escape latency of the rats in Des group was significantly prolonged;the spatial exploring time (29.85 ± 4.51) s was reduced;the apoptotic rate of neurons (0.742 ± 0.052)％,the amount of Aβ1-42 peptide (9 618.72 ± 1 076.43) pmol· g-1,the expression levels of Caspase-3 (1.132 ± 0.217),and Bax (1.298 ± 0.209) were increased;the expression of Bcl-2 (0.318 ±0.038) were reduced;the activity of acetylcholinesterase (96.38 ±7.62) U·mL-1 was increased.Compared with the Des group,the rats in all Chr groups obtained shorter escape latency and longer spatial exploring time;the amount of Aβ1-42 peptide and the expression levels of Caspase-3 and Bax were down-regulated;the activity of acetylcholinesterase was reduced.In addition,chrysophanol improved the abilities of learning and memory of anesthetic rats in a dose-dependent manner.Conclusion Chrysophanol could improve the abilities of the learning and memory of rats after desflurane anesthesia,along with inhibition of Aβ deposition.

Objective To investigate the expression and clinical significance of high-sensitivity C-reactive protein(hs-CRP) and interleukin-6 (IL-6) in patients with recurrence of atrial fibrillation(AF) after radiofrequency catheter ablation.Methods Retrospective analysis was made among a total of ninety-seven cases of paroxysmal atrial fibrillation treated by radiofrequency catheter ablation in Chaoyang Central Hospital from January 2011 to December 2015.The patients were divided into recurrent group and successful group,depending on whether they had recurrence in six months after radiofrequency catheter ablation.The concentration of the patients'' serum hs-CRP and IL-6 before radiofrequency catheter ablation were detected and compared with those of the healthy subjects (control group) during the same period.Logistic regression analysis was used to analyze the risk factors in atrial fibrillation recurrence after radiofrequency catheter ablation.Results The levels of serum hs-CRP was (4.6±1.2) mg/L and IL-6 was(0.19±0.05) mg/L in the recurrent group,higher than those in the control group ((2.0±0.3) mg/L,(0.10±0.03) mg/L).The differences between the two groups were statistically significant (P=0.037,0.046).According to the atrial fibrillation recurrence,97 patients were divided into recurrent group (29 cases) and successful group (68 cases).The levels of serum hs-CRP and IL-6 in the recurrent group were (5.61±2.30) mg/L,(0.30±0.07) mg/L,respectively,while the data of the successful group were (3.29±1.62) mg/L,(0.15±0.06) mg/L,respectively.There were significant differences between the two groups (P=0.047,0.036).Logistic regression analysis showed that the left ventricular anterior and posterior diameter (OR=2.073,95%CI:1.078~4.126,P=0.031),levels of serum hs-CRP (OR=1.943,95%CI:1.025-3.698,P=0.042) and serum IL-6 (OR=2.071,95%CI:1.089-10.101,P=0.027) were all risk factors in atrial fibrillation recurrence after radiofrequency catheter ablation.Conclusions Inflammatory factors might be involved in the occurrence and maintenance of atrial fibrillation.The expression levels of serum hs-CRP and IL-6 are related to the recurrence of AF,which could be independent predictors of postoperative atrial fibrillation recurrence.

Objective:To explore the efficacy of iodized lecithin in treatment of macular edema. Methods:From February 2014 to February 2015,a total of 66 patients(66 eyes) with macular edema in our hospital were divided into control group(n=33) and ob-servation group ( n = 33 ) randomly. Iodized lecithin combined with conventional therapy was applied in observation group, and conventional therapy was applied in control group. Observed the change of best corrected visual acuity( LogMAR BVCA) and central macular thickness(CMT) in the two groups pre and post treatment,and observation on clinical curative effect of the two groups at 6 month after treatment. Results:Compared with pre-treatment,the average value of LogMAR BVCA and CMT in the two groups showed significantly decreased at 1, 2, 3, 6 months after therapy ( P< 0. 05 ) . Compared with control group, the observation group could significantly decrease the average value of LogMAR BVCA and CMT at 2,3,6 months after therapy (P< 0. 05). After 6 months of treatment,the total effective rate of observation group showed significantly higher than control group (P< 0. 05). Conclusion: Iodized lecithin combined with conventional therapy have a significant curative effect to improve vision,promote regression of macular edema, and enhance the treatment effect. It’s worthy of clinic application.

OBJECTIVE:To establish a method for the simultaneous determination of andrographolide,quercetin-3-O-gluco-side,dehydroandrographolide and kaempferol-3-O-glucoside in Compound chuanxinlian tablet. METHODS:HPLC was performed on the column of Phenomenex C18 with mobile phase of acetonitrile- 0.1% phosphoric acid aqueous(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 225 nm for andrographolide,335 nm for quercetin-3-O-glucoside and 254 nm for de-hydroandrographolide and kaempferol-3-O-glucoside,column temperature was 25 ℃,and the volume injection was 10 μl. RE-SULTS:The linear range was 0.111-2.770 μg for andrographolide(r=0.999 9),0.064-1.605 μg for quercetin-3-O-glucoside (r=0.999 9),0.086-2.138 μg for dehydroandrographolide(r=0.999 9)and 0.050-1.260 μg for kaempferol-3-O-glucoside(r=0.999 9);the limits of quantitation were 0.48 ng,0.88 ng,0.93 ng and 1.03 ng,the limits of detection were 0.79 ng,1.15 ng,0.96 ng and 1.07 ng,respectively;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 96.57%-100.30%(RSD=1.3%,n=9),95.04%-98.87%(RSD=1.3%,n=9),95.74%-102.92%(RSD=2.4%,n=9) and 96.73%-100.95%(RSD=1.6%,n=9),respectively. CONCLUSIONS:The method is sensitive,reliable and reproducible,and can be used for the si-multaneous determination of andrographolide,quercetin-3-O-glucoside,dehydroandrographolide and kaempferol-3-O-glucoside in Compound chuanxinlian tablet.

It is generally accepted that Kasai operation is the best treatment for biliary atresia (BA). The implementation of early screen plan improves the age threshold at the suitable time point of Kasai operation. This is the key issue to improve the survival rate of autologous liver in biliary atresia. The monitoring of stool color card, B-ultrasonography and serum con?jugated bilirubin can be used for preliminary seeming biliary atresia. It is very important to improve the ability of basic unit medical staff for major improvements to the understanding of the significance of biliary atresia. In some country and district, the local government has put the screen list for BA into healthcare booklet for children. Thus, it has a significant impact of early screen plan on the diagnosis and treatment of BA, and it can improve the BA autologous liver survival time. So far, there is still no BA early screen plan in the nationwide. We should pay attention to BA, and establish early screen system in different local health unit quickly.