Objective To study the technique of using the postauricular revolving door island flap in one-stage reconstruction of concha defect and its efficacy.Methods Fifteen cases of concha de fect were repaired by the postauricular revolving door island flap in one stage reconstruction from 2000-2010.The maximum size of defect was 2.7 cm× 3.0 cm,and the minimum was 1.0 cm×1.3 cm.Results All the island flaps were survived postoperatively,and satisfactory in auricular appearances of all patients by 6 months to 5 years follow-up.Conclusions The revolving door island flaps with abundant blood supply are transferred to reach the auricular defect,which match normal auricular tissue in contour,color,and texture. This flap on the retroauricular area is relatively concealed,and it therefore can be one of the best procedures for repair of the auricular defects,especially concha.

Ascorbic acid was found to inhibit the activity of collagenase in vitro significantly at the concentration of 56.7mmlo/L and completely at the concentration of 85.0mmol/L. This effect was destroyed by heat. The experiment explained further the useful action of topical ascorbic acid in the treatment of corneal alkaline burn and ulcer.

OBJECTIVE:To facilitate a sound and orderly development of hospital preparations(HP).METHODS:The suggestion of classified management of HP was come up with based on the characteristics of it.RESULTS & CONCLUSIONS:It is advisable for HP be managed by dividing them into market supplement agents,cipher prescription preparation,dosage form reformation agents,sterilizing agents,diagnostic agents and supplemental agents.And the licence numbers of the first 3 categories should be gained from the provincial Food and Drug Administration,while those of the sterilizing agents,diagnostic agents and supplemental agents from the municipal Food and Drug Administration.

The paper describes the concept and general development of competitive intelligence, pointes out that carrying out this kind of service is keeping with the demand of development of library, college and university as well as society. College and university libraries have the advantages in carrying out competitive intelligence service, including abundant literature resources, advanced equipments and qualified talented person. Besides, the paper poiuts out the factors which may restrict the development and puts forward countermeasures.

Human papillomavirus is a DNA virus that can induce uterine cervical neoplasms, especially the high-risk and mixed infection. In this review, the progress on HPV feature and carcinogenic mechanisms,molecular detection on uterine cervical neoplasms and papillomavirus vaccines is discussed.

An ideal assessment is an essential aspectfor patients with rectal cancer in personal treatment management and prognosis prediction.Magnetic resonance imaging (MRI) plays a central role in this process.High-resolution MRI can be used to accurately measure the depth of extramural rectal cancer spread.Therefore,high-resolution MRI can define the tumor category of rectal cancer in accordance with American Joint Committee on Cancer/Union for International Cancer Control (AJCC/UICC).MRI can detect the extramural venous invasion that has been demonstrated as an independently predicting factor for prognosis and survival of patients.In addition,MRI defines the tumor and relevant anatomy,providing the most detail on treatment choice.

This article discussed the development, challenge and solution for the front page of inpatient medical record based on DRGs (diagnosis related groups).It will facilitate the stakeholders' understanding, unifying the standard of data, building quality control system, improving the quality of the front page of medical record.Thus, the information in the front page will contribute to the scientific evaluation of health service ability and quality for hospital.

The auxotype of 36 strains of N. Gonorrhoeae collectad in Chongqing was studied by the method of NEDA and deficient NEDA media. 9 distinct clusters were found : 2 strains of Arginine type; 3 strains of Isoleuine type; 2 strains of Uracil and Hypoxanthine type; 8 strains of Cysteine and Cystine type; 4 strains of Proline type. 4 strains of Methionine typs; 5 strains of Vitamin and Co-enzyme type; 4 strains of Multiple-deficient type; 4 strains of Zero type

Objective:To explore the optimal transfection conditions in which the concentrated virus supernatant transfer the foreign mdr-1 gene into hematopoietic cells of rabbit bone marrow,and test the expression and function of the mdr-1 gene in cells.Methods:An amphotropic virus producer cell line,PA317-HaMDR1/A1 which contains a full-length cDNA of human mdr-1 gene,was screened with colchicine(90ng/ml),and the supernatant was collected and concentrated.The bone marrow cells of the New Zealand rabbits were gathered and the mononuclear cells which contain many hematopoietic cells were enriched.The hematopoietic cells were cocultivated with concentrated virus supernatant and interleukins.The transfection rate was determined by SP immunohistochemical methods;the integration of mdr-1 gene in rabbit mononuclear cells was tested by PCR method ,and the physiological function of mdr-1 gene was tested by DNR extrusion test.Results:After screening by colchicine,the expression of P-glycoprotein(P-gp) of PA317-HaMDR1/A1 was enhanced.The foreign mdr-1 gene was transferred into hematopoietic cells of rabbit bone marrow successfully,and the transduction rate of 2 days,4days and 6 days were 22%,37%,and 39% respectively.However,the state of the 4 days was the best.The transferred mdr-1 gene had its physiological function.Conclusion:The foreign mdr-1 gene can be transferred into hematopoietic cells of rabbit bone marrow successfully by concentrated virus supernatant transfection method,and the transferring gene can be expressed stably and effectively.The study has provided a basis for the further research on chemoprotection experiment of the mdr-1 gene transferred into the bone marrow cells.

Objective:To assess their influence on position,age,pepsin in gastric juice and different diseases to aspiration of gastric contents.Methods:To choice pepsin levels in tracheal aspirates as an indicator,the own control designing was adopted in the present study.Twenty mechanical ventilated children in different diseases who were 0-8 years old were nursed prone position and 30?supine fowler position.Tracheal aspirates were harvested at 1 hour,2 hours and 3 hours in different body positions and pepsin levels in tracheal aspirates were measured.At the same time pepsin levels in gastric contents were measured.Results:At the same time pepsin levels in tracheal aspirates at prone positioning were obviously less than that at 30?supine fowler position(P0.05).Conclusion:Aspiration of gastric contents at prone positioning were smaller than that at 30?supine fowler position.Pepsin levels may generally apply in paediatrics as an indicator.