Objective To investigate the clinical value of combined detection of serum human epididymis protein 4(HE4),carbo-hydrate antigen 125(CA125)and tumor marker(CEA)in the diagnosis and staging of ovarian cancer.Methods 90 cases of ovarian cancer(ovarian cancer group),120 cases of benign ovarian disease(ovarian benign group)and 60 cases of healthy women(healthy control group)were selected from March 2014 to 2016 March in our hospital.The levels of HE4 and CA125 and CEA were detected by immunochemiluminescence assays,the results of the three groups were compared and analyzed.Results The positive rate of 3 indicators in the ovarian cancer group was significantly higher than that of the ovarian benign group and the healthy control group (P <0.05).The positive rates of combined detection of Ⅰ-Ⅳ in ovarian cancer were 81.2%,92.1%,97.4% and 100.0%,and CEA,CA125 and HE4 joint inspection in different stages of ovarian cancer positive rate is significantly higher than that of single in-dex positive detection rate.Conclusion The clinical value of HE4,CA125,CEA combined test for the diagnosis and staging of ovar-ian cancer is significant,it is worth to be further promoted.

Hepatitis B recurrence is one of the most frequent complications that will probably result the treat failure of liver transplantation. As many as 80% of patients with HBV are reinfected by this virus after liver transplantation. This has been the most deadly case that threatens patient survival amongst all problems emerging from liver transplantation executed for Hepatitis B ralated hepatocirrhosis. In recent years a great deal of prophylaxis practices have been adopted, which has greatly reduced the possibility of Hepatitis B recurrence. The present paper reviews the causes and treatment of Hepatitis B recurrence after liver transplantation.

Objective To compare methimazole and propylthiouracil affecting liver function by treating hyperthyroidism.Methods Total 80 patients with hyperthyroidism were randomly divided into two groups.Group A (41 cases) was given methimazole,group B (39 cases) was given propylthiouracil.The effect of methimazole and propylthiouracil affecting liver function by treating hyperthyroidism was evaluated by the indexes of thyroid function,liver function,liver damage,and adverse reaction during treatment.Results Before treatment,there were no statistical significance on FT3 and FT4 between two groups.After treatment,the FT3and FT4 were decreased and TSH was increased in two groups (P ＜ 0.05).But there were no statistical significance between two groups.Before treatment,there was no statistical significance on ALT,AST,and GGT between two groups.After treatment,the ALT,AST,and GGT increased in group B and higher than that of group A (P ＜ 0.05).The ALT,AST,and GGT had no change before and after treatment in the group A.During treatment,there were five cases of liver injury in group A and 14 cases in group B.The liver injury rate of group B was higher than that of group A (P ＜ 0.05) and injury time of group B was later than that of group A.During treatment,there was no statistical significance on adverse reaction rate between two groups.Conclusion The efficacy of methimazole was equal to propylthiouracil in treating hyperthyroidism.Compared with propylthiouracil,the methimazole had less injury on liver but induce the liver injury in earlier.It is suggested that the patients should check the liver function regularly during treatment.

Objective To discuss the occurrence of adverse drug reactions (ADR) induced byDanhong Injection; To provide references for rational clinical medication.Methods Literature related to ADR induced byDanhong Injection was retrieved from CNKI, Wanfang database and CBM database during Jan. 2004 to Dec. 2015. Literature was selected based on inclusion and exclusion criteria. Data were collected and analyzed by metrology.Results 116 cases of ADR induced byDanhong Injection were included. The analysis showed that there was no significant correlation between ADR and gender. Most of ADR cases occurred over the age of 50 and within 30 min after medication. Multiple organs and systems were involved in ADR. Damages in skin tissue, the whole body, and respiratory system were common. Relevant clinical symptoms were mainly itching, skin rash, fever, shiver and shortness of breath. However, anaphylactic shock was needed to be aware of.Conclusion The incidence of ADR induced byDanhong Injection requires great attention in order to standardize medication and improve safety.

Objective To investigate the related factors of encephalopathy following orthotopic liver transplantation (OLT).Methods The clinical data of 128 patients who underwent liver transplantation consecutively between October 2001 and October 2003 were analyzed retrospectively by SPSS 10.0 . All clinical factors were analyzed by Crosstabs or independent-samples t test. A mutivariative analysis of these significant factors was done by using the Binary Logistic Regression.Results Encephalopathy occurred in 21 cases within the first week after operation, including 19 cases of Child-pugh C and 2 cases of Child-pugh B. There was a higher occurrence in the patients with chronic severe hepatitis or undergoing re-transplantation ( P 0.05 ). In the encephalopathy group the incidence of renal failure after transplantation and infection pre- or postoperation was higher than in control group ( P

Objective To study the effect of thiazolidinediones on vascular endothelial function in patients with type 2 diabetes mellitus.Methods 50 patients with type 2 diabetes were randomly selected as the research objects,the representative drug pioglitazone application situation for 50 patients were measured before and after the treatment,fasting blood glucose,postprandial 2h blood glucose,total cholesterol,high density lipoprotein cholesterol, low density lipoprotein,total cholesterol,triglyceride,fasting insulin,glycosylated protein and inflammatory factors in red vascular cell adhesion factor were detected.Results the patients after treatment of endothelium dependent vaso-dilatation function improved,compared with those before treatment (P <0.05),fasting blood glucose,patients after the treatment of 2h after meal blood glucose.Conclusion The two thiazolidinediones can effectively improve type 2 diabetes vascular endothelial function,and have a positive effect on the prevention of patients with cardiovascular dis-ease.

Objective]To observe the clinical effect of chief physician Zhao Runchen’s therapy of nerve root type cervical spondylosis.[Methods]According to the random and parallel control test principles, 48 cases of patients with nerve root type cervical spondylosis were divided into treatment group and control group. The treatment group was treated with conventional massage combined with pulling therapy, and the control group was treated with conventional massage. After treatment every other day for 20 times, the clinical effect of the two groups and the visual analogue scale before and after treatment were observed.[Results]15 cases in the treatment group were cured, 6 cases improved, so the basic cure rate was 63%, and total effective rate was 88%. 11 cases in the control group were cured, 9 cases improved, so the basic cure rate was 46%, and total effective rate was 83%. Cure rate between the two groups had significant difference(P<0.01), and VAS score of both groups were significantly improved after treatment: the control group decreased significantly(P<0.05), and the treatment group showed extremely significant decrease(P<0.01). [Conclusion]Chief physician Zhao Runchen’s pulling therapy of nerve root type cervical spondylosis has a satisfactory basic cure rate.

Epilepsy is one of the common diseases in the nervous system with its complicated pathogenesis still remains unknown.The drug resistance mechanism of intractable epilepsy has always been a key point in the research of neuroscience.A possible cause for the drug resistance is the over expression of efflux drug transporters,e.g.ATP-binding cassette transporters,which may decrease extracellular antiepileptic drugs levels in brains of intractable epilepsy patients.ATP-binding cassette transporters are super family of transporter proteins that require ATP hydrolysis for the transport of substrates across membranes,including P-glycoprotein,multidrug resistance-associated protein,major vault protein and breast cancer resistance associated protein.They are major impediment for the AED successful treatment of many forms of refractory epilepsy in human.This paper reviews the research progress on over-expression of ATP-binding cassette transporters and mechanism of drug resistance in intractable epilepsy.

BACKGROUND:The amniotic membrane is the rejected material after birth. Amniotic mesenchymal stem cel s are characterized as easy harvesting, strong proliferation ability, no ethical controversy, and low immunogenicity. OBJECTIVE:To electrotransfer human telomerase reverse transcriptase (hTERT) gene into amniotic mesenchymal stem cel s transplanted into diabetic rats and to explore its effect on diabetic rats. METHODS:Human amniotic mesenchymal stem cel s were isolated, cultured and electrotransferred by hTERT gene. Ten of 50 Sprague-Dawley rats were randomized selected as controls, and the remaining rats were used to establish diabetic models through injection of 45 mg/kg streptozotocin. Thirty-six model rats were randomized into model group, cel transplantation group and hTERT-transfected cel transplantation group, with 12 rats in each group. In the latter two groups, human amniotic mesenchymal stem cel s and hTERT-transfected amniotic mesenchymal stem cel s were injected via sublingual veins, respectively. After transplantation, blood glucose levels were monitored dynamical y, and plasma insulin concentration was detected every week. Pancreas tissues were taken and cut into sections for histological observation using hematoxylin-eosin staining. RESULTS AND CONCLUSION:At 4 weeks after transplantation, the blood glucose levels were significantly lower in the two cel transplantation groups than the model group (P<0.05), and especial y in the hTERT-transfected cel transplantation group, the blood glucose level was close to the normal value (P>0.05). However, the model group stil had a higher blood glucose level. At 6 weeks after transplantation, compared with the model group, the plasma insulin concentration was significantly increased in the two cel transplantation group (P<0.05), and the severity of pancreatic injury was also eased in these two groups (P<0.05), especial y in the hTERT-transfected cel transplantation group (P<0.05). These findings indicate that hTERT-transfected amniotic mesenchymal stem cel transplantation can dramatical y decrease blood glucose level and relieve pancreatic injury in diabetic rats, which is an effective method for treatment of mel itus diabetes in rats.

Objective To analyze the clinical nusing care methods and effects of patients complicated with tuberculosis during pregnancy or puerperal state.Methods Clinical data of 39 cases of patients complicated with tuberculosis during pregnancy or puerperal state were retrospective analyzed.Results After comprehensive treatment and nursing care,4 cases died,others got good prognosis.Conclusions Meticulous treatment and nursing can promote the rehabilitation of patients complicated with tuberculosis during pregnancy or puerperal state.For these patients,the cure rate can be improved and the recurrence rate can be reduced by choosing suitable treatment and nursing care programs,paying attention to psychological care and health education,ensuring patients taking medicine regularly.