Objective:To explore the diagnostic value of the combined detection of galactomannan (Gm) in bronchoalveolar lavage fluid (BALF) and IgG of aspergillus fumigatus in blood for pulmonary aspergillosis.Methods:Seventy-four patients with pulmonary aspergillosis admitted to Haiyan People′s Hospital from February 2017 to September 2019 were divided into chronic pulmonary aspergillosis group (35 cases) and invasive pulmonary aspergillosis group (39 cases). The BALF of ttwo groups were collected, the Gm level of BALF was tested, and the IgG level of blood aspergillus fumigatus was detected by using the aspergillus IgG enzyme-linked immunosorbent assay (ELISA) kit, and the positive prediction rate, negative prediction rate, diagnostic sensitivity, specificity and accuracy of the two methods was compared.Results:The Gm I value of BALF and the IgG level of blood in invasive pulmonary aspergillosis group were lower than those in chronic pulmonary aspergillosis group: 0.65 ± 0.09 vs. 0.98 ± 0.12, (118.95 ± 12.31) kAU/L vs. (147.63 ± 15.32) kAU/L, and there were significant differences ( P<0.05). The positive predictive rate and negative predictive rate in combined detection of detection of GMI and IgG in BALF and blood were significantly higher than those in the single detection of GMI and IgG in BLAF and blood: 92.50%(37/40) vs. 61.90%(26/42) and 61.54%(24/39), 88.24%(30/34) vs. 53.13%(17/32) and 51.43%(18/35), and there were significant differences ( P<0.05). There was a certain correlation between the severity of pulmonary aspergillosis and the Gm I value of BALF and the IgG level of blood aspergillus fumigatus. The sensitivity and accuracy of the diagnosis in combined detection of Gm I and IgG in BALF and blood were higher than those in the single detection of Gm I and IgG in BALF and blood: 92.86%(39/42) vs. 65.00%(26/40) and 67.57%(25/37), 83.78%(62/74) vs. 75.68%(56/74) and 75.68%(56/74), the diagnostic specificity was lower than that of the single detection of GM I and IgG in BALF and blood: 71.88%(23/32) vs. 88.24%(30/34) and 83.78%(31/37), and there were significant differences ( P<0.05). Conclusions:There is a certain correlation between Gm in BALF and aspergillus fumigatus IgG in blood and the symptoms of pulmonary aspergillosis. The combined detection of Gm in BALF and aspergillus fumigatus IgG in blood is of great significance for the clinical diagnosis and treatment of pulmonary aspergillosis.

Objective:To analyze the prevalence and clinical features of respiratory syncytial virus (RSV) in Chengde city.Methods:From August 2022 to June 2023, throat swabs and clinical data of 478 hospitalized children with respiratory tract infection in the Chengde Central Hospital were collected. Real-time quantitative PCR was used to detect the molecular epidemiology of RSV-A and RSV-B subtypes and analyze the clinical features of patients with RSV infection.Results:Among the hospitalized children, 67.57% (323/478) tested positive for RSV. The outbreak of RSV infection was caused by RSV-A subtype. The peaks of RSV-A infection occurred from November to December, 2022 and May to June, 2023. There were 86.07% (278/323) of the RSV-A-positive cases had mixed infection with other pathogens, primarily bacterial pathogens with Streptococcus pneumoniae being the most common, followed by Klebsiella pneumoniae. Influenza virus A was the most common viral pathogens causing mixed infection. The level of lactate dehydrogenase was higher in the patients with single RSV-A infection than in those with mixed infection ( Z=2.396, P=0.017), and higher than the normal upper limit. Compared with the single infection group, the mixed infection group had higher white blood cell count ( Z=2.417, P=0.016), neutrophil ratio ( Z=3.218, P=0.001), C-reactive protein level ( Z=1.998, P=0.046) and creatinine level ( Z=2.107, P=0.035), and lower lymphocyte ratio ( Z=3.205, P=0.001), but they were all within the normal range. There were no significant differences in the clinical features between RSV-A-positive patients co-infected with bacteria or other viruses (all P>0.05). Conclusions:RSV-A is the leading cause of respiratory tract infection in children in Chengde from 2022 to 2023, and often co-detected with bacteria. The mixed infection with other respiratory pathogens is related to the clinical features of patients with RSV-A infection.