Objective To investigate the effect of TGF? 1 on expression of MHCⅠ type antigen in human pulmonary squamous cell carcinoma.Methods Expressions of ? 2 M in primary culture cells with rhINF? and rhTGF? 1+rhINF? from human pulmonary squamous cell carcinoma were detected by FCM(Flow Cytometry Method), respectively. Results Expression of ? 2 M in all tumor cell groups was induced significantly by rhINF? ( P 0.05) Conclusion Expression of INF? induced MHCⅠ type antigen in human pulmonary squamous cell carcinoma can be inhibited by TGF? 1.

Objective To investigate the expression of TGF ? 1 in human pulmonary squamous cell carcinoma Methods TGF ? 1 in serum free conditioned medium of different differentiated degree primary cells in culture from human pulmonary squamous cell carcinoma were detected by ELISA Results TGF ? 1 in serum free conditioned medium from all tumor cell groups is significantly higher than from normal bronchial epithelial cell groups ( P 0 05) Conclusion Inhibition effect by TGF ? 1 on cell growth of human pulmonary squamous cell carcinoma is weakened

Objective:To analyze the correlation of protein kinase PLK4 with the prognosis of patients with malignant glioma and the proliferation of human glioblastoma cells. Methods:The relationship of PLK4 expression to the pathological grade and prognosis was evaluated using CGGA datasets. Designated siRNAs targeting PLK4 were constructed, and the silencing effect was identified by RT-qP-CR and Western blot analyses. The effect of PLK4 on the proliferation of tumor cells was evaluated by MTT and colony formation as-says. Results:The expression of PLK4 increased with pathological grade and was significantly related to the overall survival in patients with glioma and the prognosis of patients with high-grade glioma. MTT and colony formation assay results indicated that the prolifera-tion activity was significantly reduced after depleting PLK4. Conclusion:The expression of PLK4 is related to the prognosis of patients with glioma and can affect the proliferation activity of tumor cells. Therefore, PLK4 could be a potential therapeutic target in glioma treatment.

Objective To explore the clinical features,treatment and prognosis of glioblastomas(GBM)in adults.Methods A retrospective cohort study was performed on 176 adult GBM patients admitted to our department from January 2015 to December 2021.Chi-square test was used to investigate the clinical differences between isocitrate dehydrogenase(IDH)mutant and wild-type GBM.Kaplan-Meier and Log-Rank tests were employed to plot survival curve and compute the survival analysis.Multivariate Cox regression model was applied to identify the independent prognostic factors.Results IDH wild-type GBM account for 89.2％and had significantly differences from the IDH-mutant GBM in terms of age of onset,Karnofsky(KPS)score at admission,symptoms of neurological deficit,and methylation status of O6-methylguanine-DNA-methyltransferase(MGMT)promoter(P＜0.05).For the IDH wild-type GBM patients receiving conventional therapy,univariate Cox hazard analysis showed gross total resection,methylation of MGMT promoter,initiation of radiation within the 5th to 6th week after surgery,and adjuvant temozolomide(TMZ)chemotherapy ≥6 cycles were favorable prognostic factors for overall survival(OS);GBMs in the left hemisphere,involvement of single lobe,methylation of MGMT promoter,and initiation of radiation within the 5th to 6th week after surgery were favorable prognostic factors for progression free survival(PFS)(all P＜0.05).Moreover,multivariate Cox hazard regression analysis indicated that methylation of MGMT promoter,and initiation of radiation within the 5th to 6th week after surgery,and adjuvant TMZ chemotherapy ≥6 cycles were independent protective factors for OS,and GBMs in the left hemisphere,involvement of single lobe and methylation of MGMT promoter were independent protective factors for PFS in the GBM patients(all P＜0.05).Conclusion The clinical and prognostic features are totally different between IDH mutant and wild-type GBM,and molecular detections are needed for the further pathological classification.Methylation of MGMT promoter is a primary marker of favorite prognosis for IDH wild-type GBM,and slightly delay in radiotherapy(the 5th to 6th week after surgery)can effectively improve the survival prognosis of IDH wild-type GBM.