Objective To investigate the effect of TGF? 1 on expression of MHCⅠ type antigen in human pulmonary squamous cell carcinoma.Methods Expressions of ? 2 M in primary culture cells with rhINF? and rhTGF? 1+rhINF? from human pulmonary squamous cell carcinoma were detected by FCM(Flow Cytometry Method), respectively. Results Expression of ? 2 M in all tumor cell groups was induced significantly by rhINF? ( P 0.05) Conclusion Expression of INF? induced MHCⅠ type antigen in human pulmonary squamous cell carcinoma can be inhibited by TGF? 1.

Objective To investigate the expression of TGF ? 1 in human pulmonary squamous cell carcinoma Methods TGF ? 1 in serum free conditioned medium of different differentiated degree primary cells in culture from human pulmonary squamous cell carcinoma were detected by ELISA Results TGF ? 1 in serum free conditioned medium from all tumor cell groups is significantly higher than from normal bronchial epithelial cell groups ( P 0 05) Conclusion Inhibition effect by TGF ? 1 on cell growth of human pulmonary squamous cell carcinoma is weakened

Objective:To analyze the correlation of protein kinase PLK4 with the prognosis of patients with malignant glioma and the proliferation of human glioblastoma cells. Methods:The relationship of PLK4 expression to the pathological grade and prognosis was evaluated using CGGA datasets. Designated siRNAs targeting PLK4 were constructed, and the silencing effect was identified by RT-qP-CR and Western blot analyses. The effect of PLK4 on the proliferation of tumor cells was evaluated by MTT and colony formation as-says. Results:The expression of PLK4 increased with pathological grade and was significantly related to the overall survival in patients with glioma and the prognosis of patients with high-grade glioma. MTT and colony formation assay results indicated that the prolifera-tion activity was significantly reduced after depleting PLK4. Conclusion:The expression of PLK4 is related to the prognosis of patients with glioma and can affect the proliferation activity of tumor cells. Therefore, PLK4 could be a potential therapeutic target in glioma treatment.