Objective To explore the psychology of anxiety neurosis and drug treatment efficacy.Methods 123 clearly diagnosed anxiety neurosis sufferers were randomly divided into group A(psychotherapy),group B(drug treatment) and group C(psychotherapy and drug treatment).Psychological measuring scale SCL-90 was adopted and the results were compared at pretreatment and at 8 weeks post-treatment.Results Anxiety neurosis was often accompanied with apprehensiveness,depression,personal relationship sensitivity,compulsion and somatization.Therapeutic efficacy of group C was the best and the symptom remission rates were group A 43.9%,group B 57.5% and group C 88.6%.The rate of group C was significantly different compared with those of group A and group B(P0.05).Conclusion Though drug intervention is necessary,psychotherapy and drug treatment are the best regimens of therapy for anxiety neurosis.

Objective To develop an HPLC method for the determination of chlorogenic acid in Xiao'er Yinniu Granula.Methods A Phenomenex ODS C18 column (4.6 mm?250 mm,5 ?m) was used,column temperature was at 20 ℃,methanol-0.4 %phosphoric acid water solution(20:80)was used as the mobile phase,the flow rate was 1 mL?min-1,and the detection wavelength was 327 nm.Results The calibration curve of chlorogenic acid was linear between 0.284～1.704 ?g (r=0.999 6),the average recovery and the relative standard deviation were 96.19 %and 1.16 %(n=5),respectively.Conclusion The method is simple,accurate and reproducible,by whihc chlorogenic acid can be better separated from Xiao'er Yinniu Granula.This method can be used for quality control of Xiao'er Yinniu Granula.

OBJECTIVETo explore the mechanism of glutamate (Glu)-induced PC12 cell apoptosis and the protective effect of muscone.

METHODPC12 cells were randomly divided into six groups: normal group (Normal), model group injured by glutamate (Glu), nimodipine group (Nim) and muskone groups (Mus) of high, middle and low doses. The PC12 cells were pretreated with or without different concentrations of muskone for 30 min and then exposed to glutamate. MTT assay for cell survival, flow cytometric detection of apoptotic cells, DCF assay for reactive oxygen species (ROS) and flow cytometric assay was performed to determine the mitochondrial membrane potential in PC12 cell.

RESULTPC12 cell damage, the concentrations of [Ca2+], and apoptosis induced by Glu were decreased after being administrated with paeoniflorin.

CONCLUSIONMuskone inhibited Glu-induced apoptosis in PC12 cells. The mechanism is related to inhibiting intracellular Ca2+ overload and maintaining mitochondral membrance potential.

Animals ; Apoptosis ; drug effects ; Calcium ; metabolism ; Cell Survival ; drug effects ; Cycloparaffins ; pharmacology ; Glutamic Acid ; pharmacology ; Membrane Potential, Mitochondrial ; drug effects ; PC12 Cells ; Pheochromocytoma ; metabolism ; physiopathology ; Protective Agents ; pharmacology ; Rats