Objective:To investigate the third molar horizontal and vertical drift automatically after the second molar extraction.Methods: 26 patients,aged 11～18 years old, with the indications of second morlar extraction were divided into two groups:15 cases in experimental group with the extraction of the second molar and 11 in control group without the extraction. A cephalometric radiograph of each patient was traced on before and 9 months after treatment. Vertical and horizontal drift and mesio- angulation of the third molar were measured.Results:The third molar vertical mesio-movement (mm)in experimental and control groups was 7.08?2.04 and 2.09?0.47(P

Objective To investigate the relationship between interleukin (IL)-18 and podocyte injury of lupus nephritis (LN). Methods Sixty cases of biopsy proven LN patients were enrolled into the study. Thirty cases were selected as controls. The clinical and pathological data, blood and urine samples and renal tissues were collected. The Nephrin expression was detected by immunohistochemical method and IL-18 was measured by enzyme linked immunosorbent assay. The relationship between IL-18 and the Nephrin expression, clinical and pathological indicators of LN were analyzed. Results Thirty-eight cases were in active disease and 22 cases were in inactive disease in LN group according to SLE disease activity index (SLEDAI) 2000. One-way ANOVA showed that the level of plasma and urine IL-18 in the LN groups were higher than those in the control group [(200±38) ng/ml, (18±5) ng/ml] (F=110.84, 203.09, P<0.01). Plasma and urine IL-18 level in active LN group [(565 ±128) ng/ml, (200 ±47) ng/ml] was higher than that in the inactive group [(376 ±106) ng/ml, (67 ±22) ng/ml] (P<0.01), the level of IL-18 of type Ⅳ LN was significantly higher than that of typeⅢand type Ⅴ LN (P<0.01). The expression level of Nephrin in LN groups were lower than those in healthy control group (0.28±0.02)(F=136.39, P<0.01). The expression level of Nephrin in active LN group (0.13±0.03) was lower than that in the inactive group (0.18±0.02) (P<0.01), the level of typeⅣLN Nephrin was significantly 01). The Pearson correlation analysis showed that, compared with plasma IL-18, urine IL-18 level in the LN group was not only negatively correlated with the level of Nephrin and serum C3 (r=-0.780, -0.565, P<0.05), but positively correlated with 24 h UP, erythrocyte sedimentation rate (ESR), SLEDAI and GAI (r=0.546, 0.467, 0.599, 0.634, P<0.05). Serum IL-8 level was independent of albumin (ALB), C4, C reactive protein (CRP) and CI (P>0.05), and was negatively correlated with estimated glomerular filtration rate (eGFR) (r=-0.562, P<0.05). It was positively correlated with serum creatinin, blood urea nitrogen, AI, TLAI and inflammatory cell infiltration (r=0.529, 0.482, 0.665, 0.690, 0.671, P<0.05). Conclusion IL-18 has a very close relationship with podocyte injury in patients with LN, and the uIL-18 can be a potential non-invasive detection method to monitor podocyte injury in LN patients.

OBJECTIVESTo detect telomerase activity in patients with mycosis fungoides (MF) and to study the role of telomerase in the tumorigenesis of MF.

METHODSThe technique of PCR-ELISA was employed to detect telomerase activity in 35 patients with various stages of MF.

RESULTS92.3% tumor stage of MF, 78.6% plaque stage of MF and 75.0% patch stage of MF had positive telomerase activity. The control samples had no telomerase activity. Telomerase activity in tumor stage of MF was significantly higher than that in plaque stage, while the latter was higher than that in patch stage. Telomerase activity was correlated with the stage of MF.

CONCLUSIONHigh level of telomerase activity frequently occurred in patients with MF, suggesting that telomerase might play an important role in the tumorigenesis of MF and is a useful marker for the diagnosis of MF possibly.

Humans ; Mycosis Fungoides ; enzymology ; pathology ; Neoplasm Staging ; Skin Neoplasms ; enzymology ; pathology ; Telomerase ; metabolism

Objective To investigate the relationship between white blood cell count (WBC) and the prevalence of simple fatty liver disease (SFL) and nonalcoholic steatohepatitis (NASH). Methods We designed a large scale cross-sectional study in an adult population. Participants were selected from Tianjin Medical University's General Hospital-Health Management Centre. The diagnoses of simple fatty liver disease and nonalcoholic steatohepatitis were based on liver ultrasonography and serum alanine aminotransferase concentration. A total of 37507 subjects (8644 SFL and 2557 NASH) were included in this study. Multiple logistic regression analysis was used to assess whether the quartiles of WBC were associated with the prevalence of simple fatty liver disease and nonalcoholic steatohepatitis. Results After adjusting for potential confounders, the odds ratios (95% confidence interval) of simple fatty liver disease and nonalcoholic steatohepatitis for increasing quartiles of WBC were: simple fatty liver disease, 1.00 (reference), 1.37 (1.24, 1.50), 1.70 (1.55, 1.86) and 2.09 (1.90, 2.29) (P for trend<0.0001);nonalcoholic steatohepatitis, 1.00 (reference), 1.39 (1.16, 1.66), 1.69 (1.43, 1.99) and 2.13 (1.81, 2.50) (P for trend<0.0001). Conclusions This study proves the correlation between WBC and the prevalence of simple fatty liver disease and nonalcoholic steatohepatitis. Further study is needed to clarify whether WBC has a predictive value for the occurrence of nonalcoholic fatty liver disease.

OBJECTIVETo finish the work of sequencing the full sequence of intron 51 of dystrophin gene and understand its characteristic of sequence.

METHODSThe whole intron 51 was sequenced by primer walking. The sequencing results were analyzed by repeat sequences, matrix attachment region (MAR) and topoisomerase II cleavage sites. The residue sequences, after removal of the repetitive sequences, were subjected to the analysis of CpG islands, promoter, open reading frame (ORF) and unidentified low copy repeat sequence.

RESULTSThe acquired intron 51 sequence was composed of 38725 bp. Repetitive sequences constituted 37.53% of total intron sequence. The overall G+C content of intron 51 was 36.34%. There are four potential MARs in intron 51. Three of them are clustered in the 12 kb region near exon 51. Numerous ORFs were found on both strands, but no homologues proteins were found in Genbank CDS transcriptional peptide, PDB, SwissProt, PIR and PRF databases.

CONCLUSIONThe expansion of intron 7 over the last 120 million years was mainly the result of L1 insertion into intron 7, and not all of repetitive sequences are associated with chromosomal rearrangement. No sequence of functional significance was found in intron 51. The results suggest that the cluster of MARs may be associated with the instability of intron 51.

Base Sequence ; CpG Islands ; genetics ; Databases, Nucleic Acid ; Dystrophin ; genetics ; Gene Deletion ; Genes ; Humans ; Introns ; genetics ; Long Interspersed Nucleotide Elements ; genetics ; Mutagenesis, Insertional ; Open Reading Frames ; Promoter Regions, Genetic ; Repetitive Sequences, Nucleic Acid ; genetics ; Sequence Analysis, DNA ; methods

The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a catastrophic impact on human life and economic life. Due to the combination of multiple underlying diseases and low vaccination rates, dialysis patients are susceptible to SARS-CoV-2 infection and are likely to have more severe illness and even death. Moreover, dialysis patients with SARS-CoV-2 infection may initially present as asymptomatic or with mild symptoms, which makes it very difficult to identify severe patients at an early stage. Here, the epidemiology, clinical characteristics, risk factors for prognosis, vaccination and therapeutic strategies of dialysis patients with SARS-CoV-2 infection were summarized and analyzed, and it is hoped to provide a reference for the diagnosis and treatment of SARS-CoV-2 infection in this special group of patients.

Objective: To study the effects of long non-coding RNA linc-01135 on the osteogenic differentiation of inflammatory PDLSCs(P-PDLSCs) under 12％ static mechanical strain loading. Methods: Cells were isolated and cultured from the healthy and periodontitis samples respectively to obtain healthy PDLSCs(H-PDLSCs) and P-PDLSCs. RT-PCR were used to identify the expression level of linc-01135. Lentiviruses were used to upregulate and downregulate the expression of linc-01135, and the osteogenesis gene expression were analyzed by RT-PCR and the osteogenesis differentiation capability were evaluated by alizarin red staining. Results: linc-01135 expression in P-PDLSCs was lower than that in H-PDLSCs. When the expression of linc-01135 was upregulated in P-PDLSCs before 12％ SMS loading, the expression level of RUNX2, ALP, OPG was significantly increased. In contrast, the expression of RUNX2, ALP, OPG was significantly decreased when the expression of linc-01135 was suppressed. Alizarin red staining proved the same trend. Conclusion: linc-01135 can promote the osteogenic differentiation capability of P-PDLSCs under 12％SMS loading.

Objective:To investigate the efficacy and safety of SIMI mouthguard paint(test group) in the treatment of enamel demineralization during orthodontic therapy with fixed applince.Methods:152 cases underwent orthodontic therapy with fixed applince were included in a randomized,open,positive control trial,and were treated by SIMI and Duraphant fluoride toothpast (control group) respectively(n =76).The enamel opaque spot was observed before and 3 months after using the products.The oral mucosa reactions,asthma attacks or stomach nausea and other adverse events were recorded.Results:150 cases (n =75) completed the trial.The results showed that the test group was non-inferior compared with the control group.No adverse event was found in both groups.Conclusion:SIMI mouthguard paint is effective in control of enamel demineralization during orthodontic therapy with fixed applince.

Objective To study stress distributions of lower dentition distally moved with miniscrews in external oblique line area when the traction hooks with different lengths were placed at different positions by the three-dimensional (3D) finite element method. Methods Based on cone beam computed tomography (CBCT) data from a patient, traction hooks with the heights of 2 mm and 7 mm were constructed and fixed between the lateral incisors and the canine brackets, as well as between the canines and the first premolar brackets. Four kinds of 3D finite element models of full dentition distally moved with miniscrews were constructed. The 3 N traction force was loaded between the traction hook and the miniscrews. The maximum von Mises stress and initial displacement of the mandibular teeth and dentition under four working conditions were analyzed. ResultsWhen the traction hook with 7 mm-height was placed between the lateral incisor and the canine, the lower central incisor rotated clockwisely, with the crown moving labially and the root moving lingually. But under the other three working conditions, the central incisor rotated counterclockwisely, and the crown and root moved lingually. When the traction hook with 7 mm-height was placed between the canine and the first premolar, the lower canine crown moved proximally. But under the other three working conditions, both the crown and root moved distally. Under four working conditions, the mandibular molars all rotated counterclockwisely,with the crown and root moving distally. Conclusions Through 3D finite element analysis, biomechanical mechanism of the effect of different traction hooks on mandibular tooth movement during distalization of the lower dentition was elucidated.With full understanding of the biomechanical mechanism, proper selection for length and placement of the traction hook can achieve distal movement of the entire mandibular dentition more efficiently.

The blood-brain barrier (BBB) is a tight boundary formed between endothelial cells and astrocytes, which separates and protects brain from most pathogens as well as neural toxins in circulation. However, detailed molecular players involved in formation of BBB are not completely known. Dentin matrix protein 1 (DMP1)-proteoglycan (PG), which is known to be involved in mineralization of bones and dentin, is also expressed in soft tissues including brain with unknown functions. In the present study, we reported that DMP1-PG was expressed in brain astrocytes and enriched in BBB units. The only glycosylation site of DMP1 is serine89 (S89) in the N-terminal domain of the protein in mouse. Mutant mice with DMP1 point mutations changing S89 to glycine (S89G), which completely eradicated glycosylation of the protein, demonstrated severe BBB disruption. Another breed of DMP1 mutant mice, which lacked the C-terminal domain of DMP1, manifested normal BBB function. The polarity of S89G-DMP1 astrocytes was disrupted and cell-cell adhesion was loosened. Through a battery of analyses, we found that DMP1 glycosylation was critically required for astrocyte maturation both in vitro and in vivo. S89G-DMP1 mutant astrocytes failed to express aquaporin 4 and had reduced laminin and ZO1 expression, which resulted in disruption of BBB. Interestingly, overexpression of wild-type DMP1-PG in mouse brain driven by the nestin promoter elevated laminin and ZO1 expression beyond wild type levels and could effectively resisted intravenous mannitol-induced BBB reversible opening. Taken together, our study not only revealed a novel element, i.e., DMP1-PG, that regulated BBB formation, but also assigned a new function to DMP1-PG.
Animals ; Astrocytes ; cytology ; metabolism ; Blood-Brain Barrier ; cytology ; metabolism ; Cells, Cultured ; Extracellular Matrix Proteins ; genetics ; metabolism ; Female ; Glycosylation ; Male ; Mice ; Proteoglycans ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction