ObjectiveTo investigate the protective effects of epigallocatechin gallate (EGCG) in a mouse model of Parkinson's disease induced by 1-Methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP).Methods32 C57BL/ 6 male mice were randomly divided into 4 groups: Model group was administrated with 16 mg/kg MPTP (i.p., four times, 2 h interval); Sham group was treated with saline; EGCG treatment group was given EGCG (5 mg/kg) after MPTP administration; normal group was just given EGCG (5 mg/kg) as treatment group. After given EGCG for 3 weeks, behavioral tests, tyrosine hydroxylase (TH) immunohistochemistry staining and the HPLC for dopamine (DA) and its metabolites were used.ResultsThe present results indicated that oral administration of EGCG significantly improved the behavioral impairement in mice induced by MPTP (P<0.05). And in the EGCG treatment group, there were more TH-positive neurons than in model group. In addition, levels of DA and its metabolites in striatum decreased significantly in MPTP group (P<0.05). Though the concentration of DA and its metabolites in EGCG treatment group tended to increase, however, there was no significance between EGCG treatment and model group.ConclusionEGCG could improve the behavioral impairment in a mouse model of Parkinson's disease induced by MPTP and protect against the loss of the dopamine neurons in the substantia nigra (SN).

Objective To evaluate the application effect of clinical pharmacy services in the secondary prevention ot stroke.Methods Hospitalized stroke patients were selected in Taihe Hospital from June 2013 to August 2014.The patients meeting the inclusion criteria were paired and sequentially numbered,and randomly divided into observation group (n =100) and control group (n =102).The patients were treated routinely during thc hospitalization.The clinical pharmacists conducted the drug education.The control group implemented the telephone follow-up for guiding compliance behavior one month after discharge.The observation group continued to perform clinical pharmacist-leading service projects.The medication compliance (Morisky scale),systolic blood pressure (SBP),diastolic blood pressure (DBP),glycated hemoglobin (HbA1 C),serum total cholesterol (TC),low density lipoprotein-C (LDL-C) and relapse rate were compared three months,six months and twelve months after the two groups discharged.Results The medication compliance,SBP,DBP,HbA1C,TC,LDL-C and recurrence rate after hospital discharge were no statistically significant (P ＞ 0.05) three months after the two groups discharged,but statistically significant between the two groups (P ＜ 0.05 or P ＜ 0.01) six months and twelvc months after the two groups discharged.Levels of SBP,DBP,HbA1 C,TC and LDL-C in the two groups were both significantly lower than before (P ＜ 0.05 or P ＜ 0.01).The relapse rate of the observation group three months,six months and twelve months after they discharged was 2.00％,5.00％ and 15.00％,respectively,and that of the control group was 2.94％,13.72％ and 28.43％,respectively.Conclusion The pharmacy services in the secondary prevention of stroke can improve the patient medication compliance and better control the blood pressure,blood glucose and blood lipid lcvcls,and reduce the relapse rate.It should be recommended in the clinical practice work.

Objective To study the characteristics of injuries in patients,and provide basic evidence for injury prevention and control.Methods 3 hospitals were sampled by stratified sampling,and the data of all injured patients visiting the 3 hospitals in 2006～2007 were analyzed.Results 11 142 injured patients were collected.The ratio of male to female was 1.81∶1.The average age was 34.56?16.Injured patients were mainly peasants(40.04%) and students(20.62%).Most injuries occurred at home and highway,and at 9～10AM and 3～4PM.The cause of injuries were fall(32.12%),traffic accidents(20.33%) blunt force(16.60%),and animal attacks(11.67%).Unintentionally injured patients accounted for 87.70% of all cases.Conclusions The occupation injury,traffic injury and injury to young adolescents are seriously endangering the health of peasants and students.

Objective To investigate the effects of octacosanol on the behavioral changes and its potential mechanism in 6-hydroxydopamineinduced Parkinsonism rats. Methods 6-hydroxydopamine-induced Parkinsonism rats accepted octacosanol orally in the dosage of 17.5mg/kg (low dose), 35 mg/kg (medium dose) and 70 mg/kg (high dose) for 2 weeks, and then assessed with rotating test and narrow beam test. The apoptosis cells were counted with TUNEL assay, and the expression of nerve growth factor (NGF), precursor of nerve growth factor (proNGF), as well as their receptors were detected with Western blotting. Results The achievement of behavioral tests significantly improved after administration of octacosanol (P<0.05), with the decrease of the apoptotic cells, more expression of NGF and its receptors TrkA, and less expression of caspase-3, proNGF and its receptors p75NTR and sortilin, especially at the dosage of 70 mg/kg (P<0.05). Conclusion Octacosanol may protect the neurol impairment from 6-hydroxydopamine through NGF mediated pathway to decrease the apoptosis.

Objective To explore the effects of octacosanol on the behavioral impairments in rats with Parkinson's disease (PD) inducedby 6-hydroxydopamine (6-OHDA). Methods The SD rats were divided into the control group (n=15), the model group (n=15), the low dosegroup (n=12), the medium dose group (n=12) and the high dose group (n=12). 6-OHDA was stereotactically injected into the right striatumof the rats at 2 sites to produce PD models. The treatment groups received octacosanol with the dose of 17.5 mg/kg, 35 mg/kg or 70 mg/kgfor 2 weeks. They were tested with apomorphine-induced rotation test, the modified Morris Water Maze, and rotarod test. Results The contralateralrotation in 30 min and escape latency were less in the medium and high dose groups than in the model group (P<0.05); the latencyand total time in the rotarod test were significantly less in all the treatment groups than in the model group (P<0.01). Conclusion Octacosanolcan decrease the impaired behaviors of rats with PD induced by 6-OHDA.

Objective To investigate whether octacosanol would attenuate neurotoxicity in 1-Methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP)-treated C57BL/6N mice and its potential mechanism. Methods Behavioral tests, Nissl histochemistry and Western blot were used to investigate the effects of octacosanol in this mouse model of PD. Results Oral administration of octacosanol (100 mg/kg) significantly improved behavioral outcome in mice induced by MPTP and markedly ameliorated morphological appearances of neuronal cells in striatum. Furthermore, octacosanol blocked MPTP-induced phosphorylation of p38MAPK and JNK, but not ERK1/2. Conclusion The protective effects afforded by octacosanol might be mediated by blocking the phosphorylation of p38 MAPK and JNK on the signal transduction in vivo.

Objective To study the correlation between pain, lower limb strength and physical function in patients with primary osteoporosis. Methods Fifty-seven female patients diagnosed with menopause-related low bone mass or primary osteoporosis using a GE calcaneus bone density detector were involved in this study. The muscle strength of their lower limbs was tested with a Biodex system 4 machine. Pain was assessed with a visual analogue scale, and physical function (PF) with the SF-36 instrument. Results Low back pain was significantly correlated with PF, and so was leg pain. Leg pain was also significantly correlated with the strength of the extensors of the dominant leg during low velocity and medium velocity movement. Leg pain was not, however, significantly correlated with the strength of the flexors of the dominant leg during low and medium velocity movement. Conclusions Pain predicts poor physical function in patients with primary osteoporosis or low bone density. During low and medium velocity movement, leg pain significantly predicts poor muscle strength in the extensors of the dominant lower leg, but it has no correlation with the muscle strength of the flexors.

OBJECTIVETo observe the clinical efficacy and safety of Corydalis composite (CDC) combined with methotrexate (MTX) in treating rheumatoid arthritis (RA).

METHODSSeventy-six RA patients were randomly assigned to 2 groups, 37 in the treated group received the combined therapy, and the 39 received MTX treatment alone, all were treated for 12 weeks. Efficacy of treatment was evaluated adopting the standard of American College of Rheumatology (ACR), taking ACR20 as the chief criterion; ACR50, ACR70 as well as the clinical indexes and items in Health Account Questionnaire (HAQ) as the auxiliary criteria, including joint swelling index, joint tenderness index, holding power, morning stiffness time, resting pain, erythrocyte sedimentation rate (ESR), C-reactive protein. And the adverse reaction was recorded at the same time.

RESULTSAfter being treated for 4, 8 and 12 weeks, the ACR20 response rate reached 35.14%, 59.46% and 70.27% respectively in patients of the treated group, while that in the control group was 17.95%, 35.90% and 46.15% respectively, significant difference between groups was shown in the outcome of week 8 and 12 (P < 0.05). ACR50 and ACR70 improving rate at all the time points of observation were increased in the treated group, with the ACR50 improving rate at week 12 higher than that in the control group (43.24% vs. 20.51%, P < 0.05). As compared with the control group, the improvements in all the auxiliary criteria were more significant in the treated group (P < 0.05). The incidence of adverse reaction was less in the treated group than in the control group (32.43% vs. 56.41%, P < 0.05), particularly in term of the damage on liver (0 vs. 10.26%, P < 0.05).

CONCLUSIONCDC combined with MTX is more effective than MTX alone in treating active RA with less adverse reaction.

Adult ; Antirheumatic Agents ; administration & dosage ; therapeutic use ; Arthritis, Rheumatoid ; drug therapy ; Biological Products ; therapeutic use ; Corydalis ; Drug Therapy, Combination ; Female ; Humans ; Male ; Methotrexate ; administration & dosage ; therapeutic use ; Middle Aged ; Treatment Outcome

This paper deals with the manufacturing state of the art of biochip, and introduces a new method--laser microtechnology, including its developing procedure, characteristics and function in biochip production.
Biosensing Techniques ; instrumentation ; methods ; Computer-Aided Design ; Equipment Design ; Lab-On-A-Chip Devices ; Lasers ; Microchip Analytical Procedures ; methods ; Micromanipulation

Objective:To examine the potential influence factors of abdominal aortic aneurysm (AAA).Methods:A 1∶2 pair-matched, case-control study was conducted from July 2011 to December 2012 .A pair was composed of one AAA patient recruited from the Vascular Surgery Department , Chinese PLA General Hospital and two gender-and age-matched non-AAA subjects , one from the same hospital and the other from the community in Fangshan District in Beijing .Demographic data , medical history and the lifestyle of each subject were collected .Moreover , all the participants underwent abdominal ultra-sound or computed tomography ( CT ) and peripheral venous blood samples were obtained .Results:There were 155 case/control pairs .The multivariate conditional logistic regression model confirmed that suffering from hypertension conferred a 1.98-fold (95%CI 1.12-3.18) increased likelihood of AAA. Smoking was a strong independent risk factor of AAA , with odds ratios ( 95% confidence intervals ) of 5.23 (2.44-11.23).Dyslipidemia(OR=2.61,95%CI 1.45-4.70), a higher level of serum hs-CRP (OR=2.43,95%CI 1.37-4.31) and homocysteine (OR=2.73,95%CI 1.61-4.65) were all asso-ciated with AAA.Conclusion: Hypertension and smoking are the risk factors of AAA .Dyslipidemia, hsCRP and Hcy are associated with AAA .