1.Molecular cloning of mouse peroxisome proliferator activated receptor ?_2 and characterization of its expressing product in eukaryotic cells
Xiangsheng ZUO ; Guo LI ; Ming LUO
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Objective To provide an approach to research of peroxisome proliferator activated receptor (PPAR) ? 2 function, mouse PPAR? 2 (mPPAR? 2) gene was cloned and its transient expression in eukaryotic cells was carried out. Methods mPPAR? 2 mRNA from epididymis fat pad of Chinese Kunming mice was amplified by RT PCR and subcloned into plasmid pcDNA3 to generate the recombinant plasmid pcDNA3/mPPAR? 2 which was confirmed to contain the amplified target gene segments with fluorescence sequencing. The recombinant plasmid pcDNA3/mPPAR? 2 was used to transfect COS 7 with lipofectamine and the expressing product was detected with immune fluorescence assay and Western blot. Results The sequencing results for amplified target gene showed that the sequence of mPPAR? 2 from epididymis fat pad of Chinese Kunming mice is similar to that of mouse PPAR? 2 in Genbank, only at the site of 383 amino acid where Ser (AGC) substitutes Asn (AAC). pcDNA3/mPPAR? 2 was efficiently expressed in eukaryotic cells in vitro. Conclusion This work is the experimental basis for further researching on PPAR? 2 function.
3.Preparation,physicochemical properties and anti-tumor activity of polymeric micelles of one gemcitabine lipid derivative
Jing ZUO ; Ming YANG ; Miao LI ; Lina DU ; Yiguang JIN
Chinese Journal of Pharmacology and Toxicology 2014;(3):408-414
OBJECTIVE Topreparealipidderivativeofgemcitabine(Gem)anditspolymericmi-celles to overcome the disadvantages of Gem.METHODS N-benzyl-3′-acetyl-gemcitabine(BAG)was synthesized.A BAG-loaded poloxamer polymeric micelle (BAG∶poloxamer 188 =10∶1 ,mol/mol)was prepared using an injection method.The micelles were characterized with a laser particle size and elec-tric charge instru ment and negatively-stained trans mission electron microscopy.Hu man breast cancer cells MCF-7 were cultured with Gem or BAG polymeric micelles of 5,10,20,30,50,70,90 μmol·L-1 for 24,48 and 72 h,respectively.The inhibitory rate of cells was measured with an MTT method.The MCF-7 cytotoxicity of BAG polymeric micelles was investigated.A pharmacodynamic study was per-formed on the mice bearing mouse hepatocellular cancer cells H22.Intravenous (iv)and oral (ig)ad-ministration was used at the dose of Ge m 40 mg·kg -1 or BAG polymeric micelles 62 mg·kg -1 .The mice were administered on the 1 st,4th and 7th day and sacrificed on the 8th day.Tumor inhibitory rates were measured.RESULTS TheBAGstructurewasidentifiedbythinlayerchromatograph,1Hand13C NMR,infrared ray chromatograph and mass spectrum.The appearance of BAG micelles was a slightly blue suspension.The micelles were spheres according to the electron microscopic observation.Their size was 62.82 nm and the zeta potential was -18.8 mV.The half inhibition concentration (IC50)of Gem and BAG polymeric micelles was 40.6 and 90.0 μmol·L-1 ,5.0 and 14.9 μmol·L-1 ,5.0 and 1 3.6 μmol·L-1 at 24,48 and 72 h,respectively according to the MTT results.According to the in vivo results,compared with the tumor model group,Gem (ig),Gem (iv)and BAG polymeric micelles (iv and ig)had significant effect on the tumor weight of H22 cell xenograft mice (P<0.01 ).As for anti-tumor efficiency,BAG polymeric micelles (ig)were better than Gem (ig)(P<0.05);BAG polymeric micelles (iv)were better than BAG polymeric micelles (ig)(P<0.05),and BAG polymeric micelles (iv)were almostequaltoGem(iv).CONCLUSION ThelipidderivativeofGemcanbeloadedinthepoloxamer 1 88 polymeric micelles.BAG polymeric micelles show in vitro MCF-7 cell inhibition and in vivo inhibition of mouse H22 xerografts;iv or ig.BAG polymeric micelles (ig)show better anti-tumor effect than Gem (ig),indicating that BAG polymeric micelles are a promising novel anti-tumor oral preparation.
4.Expression of CXCL12-CXCR4 and its association with angiogenesis in pancreatic cancer.
Zuo-xing NIU ; Li-ming FEI ; Chang-liang WANG
Chinese Journal of Oncology 2009;31(4):286-287
Adenocarcinoma, Papillary
;
blood supply
;
metabolism
;
pathology
;
Adult
;
Aged
;
Carcinoma, Pancreatic Ductal
;
blood supply
;
metabolism
;
pathology
;
Chemokine CXCL12
;
metabolism
;
Female
;
Gene Expression Regulation, Neoplastic
;
Humans
;
Lymph Nodes
;
metabolism
;
Lymphatic Metastasis
;
Male
;
Microvessels
;
pathology
;
Middle Aged
;
Neoplasm Staging
;
Neovascularization, Pathologic
;
metabolism
;
pathology
;
Pancreas
;
metabolism
;
Pancreatic Neoplasms
;
blood supply
;
metabolism
;
pathology
;
Receptors, CXCR4
;
metabolism
5.Damage control surgery directed surgical treatment of Crohn disease.
Ning LI ; Wei-ming ZHU ; Lu-gen ZUO
Chinese Journal of Gastrointestinal Surgery 2013;16(4):308-310
Damage control surgery (DCS) has been widely used in the management of surgical patients. Crohn disease (CD) patients requiring surgery are usually severe and associated with high surgical risk, while the concept of DCS has not gained adequate attention in surgery for CD. Surgery is indicated in patients with CD to control symptoms, therefore major surgery should not be performed when the general health of the patients is not satisfactory. Use of DCS to guide surgery can reduce risk of treatment and improve clinical outcome The review is to discuss the necessity, objective, and methods of damage control surgery in the surgical treatment of Crohn disease.
Crohn Disease
;
surgery
;
Humans
6.Effect of surface treatment using Er:YAG laser with different parameters on the bond strength of fiber posts
Xiaojie LI ; Shuhai HU ; Ming DONG ; Nuo XU ; Xiang REN ; Enjun ZUO
Chinese Journal of Tissue Engineering Research 2014;(47):7627-7632
BACKGROUND:Recent years, fiber posts and resin cores have been widely used in repairing the endodonticaly treated teeth with satisfactory effect. Erbium:yttrium aluminum garnet (Er:YAG) laser is a new type of water power laser system, which can be used for surface treatment of fiber posts. But studies on the effect of Er:YAG laser surface treatment on the bond strength of fiber posts are rarely reported. OBJECTIVE: To evaluate the effect of surface treatment utilizing Er:YAG laser irradiation with different parameters on the bond strength of fiber posts to root canal dentin. METHODS: Fifty human maxilary central incisors that had similar dimensions were used. After endodontic treatment, removal of the crown and canal preparation, ParaPost FIBER LUX glass fiber posts were cemented into the root canals. According to the method of surface treatment, 50 teeth were randomly divided into: no surface treatment as control group; four groups undergoing surface preparation with Er:YAG laser with four different power settings (150, 250, 350 and 450 mJ at 10 Hz for 60 s at 100-μs pulse duration), named 1.5, 2.5, 3.5, and 4.5 W Er:YAG laser irradiation groups, respectively. RESULTS AND CONCLUSION: The mean bond strength values reduced from the cervical to the apical root canal, and the bond strength of the dental cervix was significantly different from that of middle and apical thirds (P < 0.05), but there was no difference between the middle and apical thirds (P> 0.05). Regardless of the different part of the root slices, the bond strength was highest in the 4.5 W Er:YAG laser irradiation group, showing significant difference from other groups (P < 0.05). These findings indicate that 4.5 W Er:YAG laser irradiation significantly increases the bond strength of the fiber posts to root canal dentin.
7.The expression and clinical significance of Livin and Smac in patients with non-Hodgkin lymphoma
Rong LI ; Xiangtao PAN ; Ye LU ; Xu CHENG ; Ming YAN ; Huizhu QIU ; Erdong ZUO
Journal of Leukemia & Lymphoma 2010;19(8):494-496
Objective To study the expression and clinical significance of Livin ( anti-apoptosis protein) and Smac (promoting apoptosis factor) in patients with the non-Hodgkin lymphoma (NHL).Methods The expression of Livin and Smac were detected by immunohistochemical staining(SP) assay in 31 patients with NHL, and the relationship between Livin/Smac and clinical staging, IPI and prognosis were analyzed. Results The patients with positive expression of Livin had B symptom, high risk IPI, late clinical staging (Ⅲ/Ⅳ stage) and short survival time, while the ones with positive expression of Smac had no B symptom, early clinical staging( Ⅰ /Ⅱ stage), low risk IPI and good prognosis. The expressions of both Livin and Smac were not related to gender and age. Expression of Livin was not correlated to that of Smac (r =0.003,P >0.05). Conclusion For patients with NHL, the expression of Livin protein was related to poor prognosis and adverse clinical features, whereas the expression of Smac protein was related to good prognosis and clinical feature.
8.Clinical value of multislice CT in diagnosis and preoperative TNM-staging of gastric carcinoma
Li-Juan DU ; Jian-Ming TIAN ; Tao-Zhen LV ; Chang-Jing ZUO ;
Academic Journal of Second Military Medical University 2000;0(07):-
Objective: To evaluate the clinical value of multislice CT (MSCT) in the diagnosis and preoperative TNM staging of gastric carcinoma. Methods: Fifty patients with advanced gastric carcinoma were examined by MSCT, gastrointestinal series (GI), fiberoptic gastroscopy (FG) and transabdominal ultrasonography (US). The results of the 4 methods were compared with postoperative pathological results. Forty patients, who were diagnosed as having advanced gastric carcinoma by both MSCT and US, had their TNM staging evaluated and the results were compared with postoperative pathological TNM evaluation. Results: The detection rates of MSCT, FG, GI and US for advanced gastric carcinoma were 98%, 100%, 88% and 80%, respectively. The detection rate of MSCT was not significantly different with that of FG, but was obviously higher than that of GI (P=0.027) and US (P=0.004). The accuracy of MSCT in preoperative TNM staging was significantly higher than that of US(92.5% vs 72.5%). Conclusion: MSCT, with two-phase thin slice incremental scanning image, multiplaner reformats (MPR) and three-dimension (3D) image, is more advantaged in detecting the gross type, size, location, invasion and metastasis of advanced gastric carcinoma, thus greatly improving the detection rate and preoperative TNM staging of advanced gastric carcinoma.
9.Effect of Chinese herb Yiyanheji on intestinal mucosa barrier in rats with severe acute pancreatitis
Jun-Shan YANG ; Yan ZUO ; Li-Ming HAN ; Yu-Zhi TIAN ; Yu HOU ;
Chinese Journal of Primary Medicine and Pharmacy 2005;0(11):-
Objective To study the effects of Chinese herbs Yiyanheji on intestinal mucosa barrier in rats with SAP.Methods Animal models of SAP were induced by retrograde injection of 5% sodium taurocholate (0.1ml/100g) into the common biliopancreatic duct.90 healthy Wistar rats weighing (250?30)g were randomly divided into 3 groups:sham operation group (SO,n=30),SAP group (SAP,n=30) and Chinese herbs Yiyanheji treated group(SAP+YH,n=30).Treated group was treated with Yiyanheji after operation.The SAP group was treated with physiological saline.Three groups of rats were killed at 72 hours after operation or treatment.Bacterial cultures were performed in all animals.The changes of terminal ileum' tissue were observed by optical electron mi- croscopy.The data of test were analyzed by statistic software.Results The incidence of bacterial translocation was 22.5% in Yiyanheji treated group,and it was lower than that in SAP group which was 90.0 %.The difference was significant(P
10.Antisense Sites Screening of Fas Gene mRNA and Its Validation in vitro
Gang ZUO ; Hui-Ming HAN ; Xiao-Li TIAN ; Quan-Hui WANG ; Jian-Ping MAO ;
China Biotechnology 2006;0(04):-
Three candidate antisense target sites of mouse Fas gene were screened by PARASS (poly-A anchored RNA accessible sites screening) technology. They were target at Fas gene 297nt-317nt, 618nt- 638nt and 662nt-682nt. Antisense oligos (A1, A2 and A3) and DNAzymes (D1, D2, and D3) for every target site were designed and synthesized. In vitro, the validation of the sites were judged by antisense oligos included RNase H splicing and the DNAzyme degradation. The results indicated that A1, A2 and A3 introduced RNase H degradation. DNAzymes D1, D2 and D3 cleaved Fas mRNA effectively. Neither degradation observed in antisense oligo RNase H group in non-target site (1211-1231nt) and 2 bases mismatched of A3, nor splicing occurred in DNzyme group in non-target site ( 1211-1231nt) and 2 bases mismatched of D3. Site 2 and 3 were at the same positions with those of ISIS Pharmaceuticals. The effective antisense oligos and DNAzymes for Fas gene could be used for the research subsequently.