1.Preparation of recombination human ?-interferon and its active analysis
Aijun ZUO ; Dongchun LIANG ; Gang GUO ; Jingyu ZHANG
Chinese Journal of Immunology 2001;0(10):-
Objective:To construct an E.coli expressing system of human interferon-?(IFN-?).Methods:Extracted DNA from human blood and PCR human IFN-?, cloned the human IFN-? gene into plasmid T-easy and pBV-220. Expressed human IFN-? in E.coli DH5?, the expressing product was analysed by SDS-PAGE, Western blot and anti-virus capacity test.Results:DNA sequence analysis showed the recombinant plasmid pBV- IFN-? contained human IFN-?. SDS-PAGE and Western blot proved that there were hIFN-? in E.coli DH5? after temperature inducing and the expressing product has anti-virus activity.Conclusion:A human IFN-? E.coli expressing system was constructed successfully, and the recombination human IFN-? has anti-virus activity.
2.Therapeutic efficacy of gemcitabine combined with cisplatin for advanced non-small cell lung cancer
Yanling ZHANG ; Zhiheng BIAN ; Wenwei ZUO ; Houjie LIANG
Journal of Third Military Medical University 2003;0(24):-
Objective To evaluate the efficacy and toxicity of the combination of gemcitabine and cisplatin in the treatment of patients with advanced non-small cell lung cancer (NSCLC). Methods A total of 30 cases of non-small cell lung cancer (stages Ⅲ and Ⅳ) were treated with gemcitabine (1 200 mg/m 2, iv infusion, at 1 and 8 d) and cisplatin (100 mg/m 2, iv infusion, at 1 d or cisplatin at 30 mg/m 2, iv infusion, at 1 and 8 d). The administration course was 28 d. Results An objective response was obtained in 46.67% of patients (2 complete and 12 partial responses), whereas 11 patients had no change and 5 patients were progressive. The response rate was 52.38% in patients with no prior chemotherapy and the response rate of 33.33% was achieved in patients who had been given prior treatment. There was significant difference between the two groups (P
3.Solitary necrotic nodule of the liver.
Zhong ZUO ; Jin-feng ZHANG ; Feng-xian TANG ; Liang FENG
Chinese Journal of Pathology 2006;35(5):317-317
Adenocarcinoma
;
complications
;
pathology
;
surgery
;
Aged
;
Colectomy
;
methods
;
Colonic Neoplasms
;
complications
;
pathology
;
Diagnosis, Differential
;
Female
;
Hepatectomy
;
methods
;
Humans
;
Liver
;
pathology
;
surgery
;
Liver Diseases
;
complications
;
pathology
;
surgery
;
Necrosis
6.Allergic reactions of COVID-19 vaccine based on mRNA-LNP and its pharmacokinetics in vivo
Chun-su LIANG ; Wei ZUO ; Li-ping DU ; Bo ZHANG
Acta Pharmaceutica Sinica 2023;57(4):867-874
Vaccination has been proved to be the most effective strategy to prevent the Corona Virus Disease 2019 (COVID-19). The mRNA vaccine based on nano drug delivery system (NDDS) - lipid nanoparticles (LNP) has been widely used because of its high effectiveness and safety. Although there have been reports of severe allergic reactions caused by mRNA-LNP vaccines, the mechanism and components of anaphylaxis have not been completely clarified yet. This review focuses on two mRNA-LNP vaccines, BNT162b2 and mRNA-1273. After summarizing the structural characteristics, potential allergens, possible allergic reaction mechanism, and pharmacokinetics of mRNA and LNP
7.Transactivation of thyroid hormone receptor βΔ on target genes through thyroid hormone responsive element
Wei XIE ; Ronglan ZHAO ; Ying ZHANG ; Aijun ZUO ; Dongchun LIANG ; Bei SUN ; Gang GUO ; Jingyu ZHANG
Chinese Journal of Endocrinology and Metabolism 2011;27(4):335-336
To study the transactivation of a novel thyroid hormone receptor isoform, TR?. pcDNA3. 1-TR? and pGL3-Promoter/thyroid hormone responsive element were co-transfected into COS-7 cells. The expression of reporter gene was detected. It could be increased up to 45 times by T3. TR? and showed the characteristics of a transcription factor.
8.An improved model of light-induced retinal damage for grading standardization in rat
Liang, TIAN ; Feng, XIA ; Lei, ZHANG ; Qun, GUO ; Jia, GENG ; Hui, CHEN ; Zuo-ming, ZHANG
Chinese Journal of Experimental Ophthalmology 2011;29(3):209-214
Background Light-induced retinal damage models vary as many influence factors,herein the modeling method is difficult to copy.It is necessary to establish the grading standardization of retinal damage after retinal light exposure.Objective This study was to improve the modeling method and establish a grading standardization for light-induced retinal damage in rat.Methods Twenty-four SPF 8-10 week-old male SD rats were randomly divided into 4 groups and 6 eyes for each group.The rats were exposed to light intense of 5000 lx for 1,2,3 hours respectively in 3 groups,and other 6 rats served as the normal group.Full-field light exposure experiment was performed for each individual rat separately,and an annular illumination box was used tO ensure the experimental rat moving in a single direction and exposing the right eye in 5000 lx light surrounding during experimental duration.Ganzfeid electroretinogram(ERG)was recorded from the experimental rats at the fifth day after light exposure,and the animals were then sacrificed for histopathology observation to evaluate the retinal thickness change.All procedures which involved animals adhered to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.Results After exposing to intensity light for 1,2,3 hours,the b-wave amplitudes of rod response,maximal mixed response,oscillatory potential in scotopic ERG as well as cone response,20 Hz flicker response of photopic ERG were significant declined as lapse of light exposure time(F=71.690,P=0.000;F=56.250,P=0.000;F=23.610,P=0.000:F=27.130,P=0.000;F=27.030,P=0.000)and lowed by 26.2%,52.5%,70.7%,24.4%,39.3%,58.1%respectively at the end of experiment.Meanwhile,the b-wave latencies of rod response,maximal mixed response in scotopic ERG as well as cone response of photopic ERG were evidently different among different groups (F=1.370,P=0.282;F:0.800,P=0.508;F=11.840,P=0.000;F=2.080,P=0.136).Light induced retinal damage located mainly at the temporal retina area.After intensity light exposure for 1,2,3 hours,the thickness of outer nuclear layer at the superior temporal retina attenuated by 11.3%,25.6%and 72.5%,respectively(P<0.05).A significant difference was seen in mean thickness of outer nuclear layer at superior temporal retina among different groups(F=410.27,P=0.000). Conclusion A standardized grading method for light-induced retinal damage is recommended.The continuous illumination in a intensity of 5000 Ix for 1,2,3 hours can induce the mild,moderate or severe retinal damage respectively at temporal retina.
9.Effect of rhBMP-2 on the Osteogenesis of Osteoblast Compounded Chitosan
Ai-Jun ZUO ; Dong-Chun LIANG ; Bao-Li WANG ; Gang GUO ; Jing-Yu ZHANG ;
China Biotechnology 2006;0(05):-
Recombinant human BMP-2 was compounded with chitosan/gelatin/hydroxyapatite(HCG) scaffold and the complex was sterilized by 60Co radiating. Osteoblast isolated from cranial bones of newborn rat was primary cultured and seeded onto the complexes. 3 days after culturing, scanning electron microscope(SEM) was applied to detect the compatibility of the cell with the complex. SEM showed osteoblast attached closely with the complex and grew well in its pores. Then the complexes with osteoblast modification were implanted into athymic nude mice subcutaneously. 8 weeks after implantation, X-ray photograph and histological observation were applied to detect the bone formation of the complexes. Under X-ray a high-density areas consistent with the shape of the implanted complex could be seen. Histological observation also proved there was bone formation in the interspace of the complex. A conclusion was drawn that rhBMP-2 compounded HCG scaffold had good osteogenesis ability in vivo.
10.Effect of probucol on adhesion of human monocytic line THP-1 induced by oxidiz ed low density lipoprotein
Chun LIANG ; Zonggui WU ; Zuo HUANG ; Renqian ZHONG ; Lingzhen ZHANG ; Li LI
Academic Journal of Second Military Medical University 2001;22(2):148-150
Objective: To determine the eff ect of probucol on adhesion of human monocytic line THP-1 induced by oxidized low density lipoprotein (oxLDL). Methods: THP-1 cells were induced by oxLDL in vitro. The CD11b, CD54 expressions and adhesion to human umbilic al vein endothelial cells (HUVEC) were measured after treatment with probucol at different concentrations by flow cytometry and β-nitrophenyl N-acetyl-β-D -glucosminide test. Results: Probucol inhibited the adhesion of oxLDL-induced THP-1 cells to HUVEC and down regulated the expression of CD11b in a dose dependent manner (P<0.01), but there was no inhibition on exp ression of CD54. Conclusion: Probucol can inhibit adhesion and a ggregation of monocyte-macrophages to endothelium in circulation, and may have anti-inflammatory action.