Objective:To evaluate the measurement uncertainty in the determination of paracetamol tablets by UV. Methods:The mathematical model of content determination by UV was established and the uncertainty sources were analyzed. Each active component of uncertainty was calculated, and the expanded uncertainty was obtained. Results:The expanded uncertainty for the UV determination of paracetamol tablets was 1. 2%,and the content determination result was (97. 0 ± 1. 2) % (k=2). Conclusion:The main sources of uncertainty are analyzed, which can provide reliable theoretical basis for the effective control of the method.

Objective To evaluate the safety and efficacy of transcatheter closure of perimembranous ventricular septal defects(VSD) using domestic symmetric double-disk occluders.Methods From March 2003 to July 2007,689 VSD patients were treated with transcatheter intervention using domestic symmetric double-disk occluders and received clinical follow up at 1 month,3 months,6months after the procedure and annually thereafter.Complications including residual shunt,heart rhythm changing,shape of the occluder and the presence of tricuspid/aortic regurgitation were observed thoroughly.Results The overall technical success rate was 98.8%.One patient died of cerebral bleeding 7 days after intervention.One patient suffered complete A-V block and required permanent pacemaker implantation and another patient conversed to open surgery because of moderate tricuspid regurgitation.Asymmetric Amplatzer occluders were used in 6 patients but the intervention failed in 2 patients whom required an open conversion.Comorbidities such as atrial septum defect,patent foramen ovale were treated concomitantly.No other complications were observed during the follow-up period.Conclusion Complication associated with transcatheter closure of perimembranous VSD using domestic symmetric double-disk occluders was relatively low.The procedure was safe and applicable.Meanwhile,it is necessary to stick to the procedural indications and avoid complications such as tricuspid regurgitation etc.

Humidificait on by artificial means must be provided when the upper airway is bypassed during mechanical ventilation.Enough humidification is very important during mechanical ventilation.Heated humidification( HH) and heta and moisture exchanger( HME) are the most commonly used types of artificial humidification in this situation.In this article, their advantages and disadvantages are itn roduced.HH na d HME are compared in humidification performance,effect no ventilator mechanics and association with venti-lation associated pneumonia.Depending on the clinical scenario, humidifier is reasonably selected, and the selection may changeo ver time according the treatment effect.

Objective:To investigate the possibility of miR-125b in serum as a novel tumor marker for primary hepatocellular car-cinoma (HCC) and the diagnosis value of combined detection of miR-125b and alpha-fetoprotein (AFP) for HCC. Methods:We detected serum miR-125b expression of 65 cases of HCC patients and 30 cases of healthy controls by real-time quantitative PCR. Moreover, we analyzed the significance of miR-125b and explored the relationship between miR-125b and clinical pathological factors. Results:The level of miRNA-125b was down regulated in serum of HCC patients compared with healthy controls which showed significant differences (P<0.05). Furthermore, the expression of miRNA-125b has no distinct correlation with sex, age, HbsAg, AFP, ALT andα-GGT, which had no significant differences (P>0.05). The expression level of miRNA-125b correlated the difference with liver Cirrhosis, tumor size and tumor node metastasis (TNM) stages, which were considered significant differences (P<0.05). The receiver operating characteristic (ROC) curve analysis of serum miR-125b for the diagnosis of HCC yielded AUC of 0.917(95%CI:0.851~0.960, P<0.001)with 85.9%sensitivity and 93.5%specificity. The ROC curve analysis of combined miR-125b and AFP for HCC detection yielded AUC of 0.951(95%CI:0.894~0.982, P<0.001)with 92.9%sensitivity and 93.5%specificity. The ROC curve analysis of serum miR-125b as biomarkers for the group (AFP<20μg/L) of HCC yielded AUC of 0.889(95%CI:0.776~0.957, P<0.001)with 84.0%sensitivity and 87.1%specificity. Conclusion:Serum miRNA-125b combined with AFP has considerable clinical value for the early diagnosis of primary HCC.

Objective To evaluate the shore-term efficacy and the X-ray evolution of new smear positive pulmonary tuberculosis after short-course chemotherapy. Methods 3229 cases with new smear positive pulmonary tuberculosis were treated with 2H_3R_3Z_3S_3/4H_3R_3 or 2 H_3R_3Z_3E_3/4H_3R_3 chemotherapy.Clinical efficacy and the X-ray evolution were analyzed after treatment. Results Among 3229 cases after treatment,the clinical cure rate and chest X-ray effective rate were 98%(3163/3229) and 98%(3163/3229) respectively. Conclusion The standardized chemotherapy treatment can achieve high clinical cure rate and X-ray cure rate for pulmonary tuberculosis.

Objective To probe the effect of applying montelukast combined with pidotimod in asthma of aged patient.Methods 120 cases of asthma in non-acute episode phase were randomly divided into three groups.Group 1 was treated with montelukast combined with pidotimod,group 2 was treated with montelukast,and the control,namely group 3 was ketotifen.All cases were treated by means of intensification therapy including glucocorticoids,?_2 agonists and some necessary anti-infection during acute episode.Results After 10 weeks treatment,both group 1 and 2 made notable improvement with respect to many asthma control parameters(P

0.05). But the amplitudes of the PhNR were significantly reduced in group of primary optic nerve atrophy (P

Measuring the content of soluble reducing sugar, total sugar, soluble protein, guanosine, alkaloids, and succinic acid of Pinellia ternata tuber were measured by anthrone-sulfuric acid colorimetric method, Coomassie brilliant blue method, RP-HPLC, reverse potentiometric titration, acid dye colorimetry, respectively. The result showed that yellow light could promote the growth and development of P. ternata and increase the content of soluble reducing sugar, total sugar, alkaloids, and succinic acid. Under blue light could promote the content of soluble protein and guanosine. Red and yellow light increased the content of chlorophyll a and chlorophyll b, contrastively blue light reduced the content of chlorophyll a and chlorophyll b. White film through the most uniform spectrum was most conducive to the synthesis of chlorophyll a. As single film, blue film, yellow film were more conducive to the synthesis of chlorophyll a, green film and red film had been relatively beneficial to the synthesis of chlorophyll b. Bulbil formed the largest number and the biggest propagation coefficient of P. ternata under red light showed that it could increase the production of P. ternata under red light.
Carbohydrate Metabolism ; radiation effects ; Chlorophyll ; metabolism ; Light ; Pinellia ; growth & development ; metabolism ; radiation effects ; Plant Leaves ; growth & development ; radiation effects ; Plant Proteins ; chemistry ; metabolism ; Quality Control ; Solubility

OBJECTIVETo observe the effect of Ganoderma lucidum decoction in treating Russula subnigricans poisoning (RSP) patients.

METHODSThe 14 patients of RSP in the treated group were treated with GLD (GLD, one dose was prepared by 100 g of Ganoderma lucidum decocted with water to 600 ml), on the base of conventional treatment, and 11 patients received conventional therapy in the previous year were taken as control. The clinical efficacy and parameters in them were compared, including the urine N-acetyl-D-glucosaminidase (NAG, which reflects the injury of kidney), the red blood cell and protein in urine, the alanine transaminase (ALT, which reflects the injury of liver), and the aspartate aminotransferase (AST, which reflects the injury of heart).

RESULTSA better clinical cure-markedly improving rate was showed in the treated group as compared with the control group, P < 0.01. In the treated group, red blood cell in urine disappeared after 24 hrs treatment in the majority of patients, urinary protein reduced obviously and the other three parameters reached the peak at the 3rd day then lowered gradually. In the control group, all the parameters increased continuously. Comparison between the parameters at corresponding time in the two groups showed significant difference (P < 0.01), those in the treated group were markedly lower than those in the control group respectively.

CONCLUSIONGLD has good effect in treating RSP, could obviously lower the fatat rate of RSP.

Acetylglucosaminidase ; urine ; Adolescent ; Adult ; Aged ; Alanine Transaminase ; blood ; Aspartate Aminotransferases ; blood ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Mushroom Poisoning ; drug therapy ; Phytotherapy ; Reishi ; chemistry

OBJECTIVETo explore the relationship between the polymorphisms of interferon-gamma (IFN-gamma) gene intron 1 at position + 874 and Condyloma Acuminata (CA).

METHODSIFN-gamma gene single nucleotide polymorphisms (intron 1 at position + 874) were detected in 156 subjects, including 76 patients with recurrent CA (CA group) and 80 healthy controls (control group), by polymerase chain reaction with sequence specific primers.

RESULTSNo significant difference of IFN-gamma 1 + 874 was found between CA group (TT, TA, and AA frequencies were 10.5%, 34.2%, and 55.3%, respectively) and control group (TT, TA, and AA frequencies were 7.5%, 30.0%, and 62.5%, respectively) (chi2 = 0.959, P = 0.619).

CONCLUSIONIFN-gamma gene polymorphism (intron 1 at position + 874) is not correlated with recurrent CA.

Adolescent ; Adult ; Aged ; Condylomata Acuminata ; genetics ; Female ; Humans ; Interferon-gamma ; genetics ; Introns ; genetics ; Male ; Middle Aged ; Polymerase Chain Reaction ; methods ; Polymorphism, Genetic ; genetics ; Polymorphism, Single Nucleotide ; Recurrence ; Young Adult