ObjectiveTo develop an assay to quantitatively detect anti-BP180NC16A IgG subclasses and to assess the significance of anti-BP180NC16A lgG in bullous pemphigoid (BP).MethodsThe Glutathione S-transferase(GST)-BPI80NC16A fusion protein was expressed in E.coli system and purified by affinity chromatography.An improved enzyme-linked immunoabsorbent assay (ELISA) was developed and used to detect anti-BP180NCI6A IgG subclasses in serum samples from 10 patients with BP,5 patients with pemphigoid gestationis (PG),1 patient with linear IgA bullous dermatosis (LIBD) and 2 patients with pemphigus.Results The optimal condition for the ELISA was determined by cross assay as follows:the concentration of GSTBP180NC16A fusion protein for coating,500 μg/L; the condition for coating,4 ℃ for 12 hours; the dilution ratio of sera and secondary antibody,1∶ 100 and 1 ∶ 2000 respectively; the condition for incubation,37 ℃ for 1 hour;,the condition for the enzyme-substrate reaction,37 ℃ for 20 minutes.Of the 10 patients with BP,all were positive for anti-BP180NC16A IgG1,9 for IgG2,5 for IgG3,and 9 for IgG4.Anti-BP180NC16A IgG was undetected in any of the serum samples from 2 patients with pemphigus vulgaris or 1 patient with adult LIBD.All the 5 sera from patients with PG; were positive for all the anti-BP180NC16A IgG subclasses,which were predominated by IgGl and IgG3.ConclusionThe developed ELISA is a highly specific and reproducible semiquantitative method for the detection of anti-BP180NCI6A IgG subclasses in patients with BP and PG.

OBJECTIVE:To find out the incidence and general patterns of drug-induced cardiovascular diseases and to guide rational use of drugs METHODS:By using literature metrological method 646 cases of drug-induced cardiovascular diseases which were reported in Chinese literatures in 1995～2000 were analysed RESULTS:Among those 646 cases,the incidence of arrhythmia was 59 8% The kinds of induced drugs were up to 155 and the most easily inducing one was cardiovascular system drug,which amounted to 37 2%(240/646) Propafenone had the highest incidence of all in inducing cardiovascular diseases(75 cases) There were 35(5 4%) fatal cases mainly caused by cardiovascular system drugs Most of the patients with drug-induced cardiovascular diseases were in middle-age and old age CONCLUSION:To reduce the rate of drug-induced diseases,cardiovascular system drugs should be rationally administrated and stictly supervised

?receptors are particularly abundant in the CNS. ? receptors have been shown to exhibit such a wide variety of actions as modulating glutaminergic, dopaminergic and cholinergic neurotransmission. They also play an important role in maintaining cell growth and proliferation, learning and memory. Recent evidence suggests the possible involvement of ? receptors in the pathogenesis of schizophrenia. More researches are expected to supply new targets for treatment and diagnosis.

Aged ; Aging ; drug effects ; Arteriosclerosis ; drug therapy ; Dementia ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Hyperlipidemias ; drug therapy ; Medicine, Chinese Traditional ; Phytotherapy ; Yang Deficiency ; drug therapy ; Yin Deficiency ; drug therapy

Objective:This study aims to explore the relationship between serum response factor (SRF) expression level and gas-tric cancer progression by detecting SRF expression level in cancer cells. Methods:The SRF gene in SGC-7901 cells was silenced by RNA interference. Transfection efficiency was detected by fluorescence microscopy, cell proliferation by CCK 8 method, SRF gene and protein expression level by real-time polymerase chain reaction and Western blot, and cell cycle by flow cytometry. Results:Cell treat-ment with siRNA-SRF induced significant reduction in SRF mRNA levels. Western blot analysis showed that SRF protein decreased by 40.1%in the siRNA group compared with that in the control group (P<0.05). Compared with the blank, negative, and mock transfection control groups, cell proliferation of the siRNA-SRF group decreased. The inhibition ratio reached 64.24%, as measured by the CCK-8 assay (P<0.05). Treatment with siRNA could block SGC-7901 cell cycle at G0/G1 phase (P<0.05). Conclusion:SRF expression is close-ly associated with gastric carcinoma cell proliferation. SRF protein level detection can provide a certain reference value in evaluating malignant gastric carcinoma progression. SRF is possibly an important target for the prevention and control of gastric cancers.

To explore the rational use of antibiotics in patients undergoing laparoscopic cholecystectomy(LC).METHODS： The antibiotic use in the selected operation patients was retrospectively studied.230 patients were divided into four groups.A group： Perioperative administration of drug； B group： Preoperative administration for 3 to 7 days and perioperative administration； C group： Perioperative administration and postoperative administration for 2 to 7 days； D group： Preoperative administration for 2 to 7 days， perioperative administration and postoperative administration for 2 to 7 days.Four groups were compared about the efficacy， length of hospital stay and hospitalization expenses.RESULTS： There were no significant differences in efficacy among A， B， C， D and in average length of hospital stay and hospitalization expenses between A and D groups.A group was the lowest in two indices and had the best cost-effectiveness ratio.CONCLUSION： There is clinical significance of preventive antibiotic application in LC patients； perioperative use is the best choice； Cefazolin is the first choice.

Based on the word-formation of Medical English terms,this paper gives a comprehensive analysis of their transformation and adaptation in Chinese language and culture.Three major factors are discussed for the application and diversification of medical English terms,including the language family in translation,linguistic context,situational context and cultural context.

OBJECTIVE:To explore the rational use of antibiotics in patients undergoing laparoscopic cholecystectomy(LC) METHODS:The antibiotic use in the selected operation patients was retrospectively studied 230 patients were divided into four groups A group:Perioperative administration of drug;B group:Preoperative administration for 3 to 7 days and perioperative administration;C group:Perioperative administration and postoperative administration for 2 to 7 days;D group:Preoperative administration for 2 to 7 days,perioperative administration and postoperative administration for 2 to 7 days Four groups were compared about the efficacy,length of hospital stay and hospitalization expenses RESULTS:There were no significant differences in efficacy among A,B,C,D and in average length of hospital stay and hospitalization expenses between A and D groups A group was the lowest in two indices and had the best cost-effectiveness ratio CONCLUSION:There is clinical significance of preventive antibiotic application in LC patients;perioperative use is the best choice;Cefazolin is the first choice

Objective To study the correlation between histopathological findings and X-ray appearances of ameloblastomas.Methods X-ray characteristics and histopathological features in 153 cases of ameloblastomas were analyzed retrospectively, and their relation was comparatively analyzed. Results ① On radiography,the lesions appeared as multilocular in 82 cases(53.59) and unilocular in 71 cases(46.41). ② 96 cases were classic ameloblastomas,including follicular type in 13 cases and non-follicular type in 83 cases,X-ray showed unilocular appearance in 35(36.46) and multilocular appearance in 61 cases(63.54).57 cases were unicystic ameloblastoma ,X-ray showed unilocular appearance in 36 cases(63.16) and multilocular appearance in 21 cases(36.84).There was significant diffrence statistically in the X-ray features of uniloculus and multiloculus between the two histopathological patterns(P﹤0.005). Conclusion Unicystic ameloblastomas often have unilocular radiologic appearance,classic ameloblastomas are mostly of multilocular radiologic appearance,while,the honeycomb radiologic appearances are common seen follicular type ameloblastoma.

Objective To discuss the mechanism of rubia cordifolia extractive affecting some biochemical indicators of blood serum and sporting ability in highly endurance-trained rats,which can provide theoretical basis for using rubia cordifolia extractive as sport supplements.Methods After establishing the highly endurance-trained rats model,we tested the exhaustion time in the trained rats and the contents of serum urea(BUN),lactic dehydrogenase(LOH),blood lactate(BLa)as well as the cruarin(Hb)in the rats.Results The activity of LDH in training group was greater than that in silent group(P