Objective To investigate the changes of platelet activating factor ( PAF) in serum from patients with hypertensive disorder complicating pregnancy .Methods The specimens from 138 women were obtained at Department of Obstetrics , Xinhua Hospital , School of Medicine , Shanghai Jiaotong Uni-versity, between June 2008 and March 2010.They were divided into two groups:women( n =63) with hy-pertensive disorder complicating pregnancy and normal pregnant women ( n =75 ) ( control group ) .All of them delivered until maturity .ELISA was used to detect the levels of PAF in those women's serum and in the umbilical cord serum .Results The levels of PAF in hypertensive disorder complicating pregnancy group (27.46 ±13.37 ) ng/ml were no significantly different from the control group (25.48 ±11.05 ) ng/ml( P >0.05 ) .With the hypertensive disorder complicating pregnancy more deteriorative , the levels of women ser-um PAF would be higher .The levels of women's serum PAF in hypertensive disorder complicating pregnancy group were significantly higher than the umbilical cord serum [0.00(0.00-8.83) ng/mL]( P <0.05). The levels of women's serum PAF in control group were also significantly higher than the umbilical cord ser -um[0.00(0.00-7.80)ng/mL]( P <0.05).Conclusion There are some relationships between PAF and hypertensive disorder complicating pregnancy .

Objective To explore the method of primary myometrial cell culture to provide a valuable cell model for a series of research works. Methods Human mometrial cells were isolated by cutting and collagenase digestion, and incubated with DMEM medium and fetal bovine serum. The first passage cells were identified by anti-desmin and-?-action antibodies immunohistochemical staining. Results Primary cultivated human myometrial cells had high purity and viability. Conclusions Primary culture human myometrial cells could obtained by cutting and collagenase digestin.

Objective To investigate the different expression of NF-κB p65 Protein in placental tissues between premature delivery and term delivery and to explore the significance of nuclear factor kB (NF-κB) protein expression in preterm delivery. Methods Fifty premature delivery pregnant women and thirty term delivery pregnant women ( were enrolled in this study. According to the way of delivery , the patients were divided into two groups. The expression of NF-κB p65 protein was analyzed by using immunohistochemistry method in the placental tissues. Results In the term delivery, NF-κB p65 was mainly negative or weak positive expressed in the cytoplasm of cells in the placental tissues. There was no demonstrable difference in immunostaining of the NF-kB p65 subunit in the placental tissues including chorion, umbilical cord and fetal membranes before and after labor ( P ＞ 0.05 ). In the premature delivery, NF-κB p65 was strongly expressed in the cytoplasm and nuclear of cells. The expression of NF-κB p65 protein in chorion and fetal membranes from premature delivery( 18% ,56% ) was significantly higher than that from normal pregnant women (6.7% ,13.3%, P ＜0.01 ). But no significant change was found for NF-κB p65 expression in umbilical cord in pregnant women with or without premature delivery ( P ＞ 0.05 ). And in fetal membranes and deciduas, there was a significant increase in the staining of immunoreactive NF-κB p65 in preterm(52.9% ,58.8% ) by caesarean section compared to tissues obtained from term delivery( 13.3%,6.7%, P ＜0.01 ). In chorion and fetal membranes, there was significant increases in the staining of immunoreactive NF-κB p65 in preterm ( 24.2%, 57.6% ) by vaginal dehvery compared to tissues obtained term delivery (6.7%, 13.3%, P ＜0.01 ). Conclusion In this study, the expression of NF-κB p65 did not show significant change in term delivery before and after labor. NF-κB p65 in premature delivery was higher than term delivery, and it had no relationship with the delivery ways.

For the case study group,52 pregnant women diagnosed as abnormal glucose metabolism and requiring in-hospital glucose control were selected for continuous glucose monitoring.And another 49 pregnant women with known glucose levels through serial glucose monitoring at the same period were taken as case control group.We divided the case study group into A1,A2 and B based on White's typing.In case study group,the glucose levels peaked at 8 to 10 am and dropped to the lowest levels at 10 pm to 6 am on the next day.The glucose of A2 group was markedly higher than that of A1 group.The highest postprandial glucose of case study group happens at (1.6 ± 0.5) h after breakfast,(1.6 ± 1.2) h after lunch and (1.6 ± 0.8) h after dinner.The blood glucose wavy coefficient of patients of A1 and A2 groups had significant statistical difference.The newborn weight of case study group was lower than that of case control group.

Objective To summarize the expreiences of management of triplet and quaternion pregnancy mothers and their in our hospital. Methods The clinical data of seven cases of multiple pregnancy (6 case of triplet and 1 case of quaternion) in our department from 1992 to 2003 were analyzed retrospectively. Results All seven patients had middle or severe anemia and hypoproteinemia in second and third trimester. The preterm sign happened in 2/3 cases in 32-36 gestation weeks. The patients were admitted in hospital in 27-34 gestation weeks because of obstetric complication. 6 triplet cases delivered during 35th-37th gestation weeks, except the quaternion one delivered at 33 gestation weeks. There were 22 newborns in those cases, the all babies Apgar's scores were above 9, the birth weights of all babies were above the 3th percent of Chinese singleton gestation and newborn estimated curves, and the birth weights of half babies were above the 10th percent position. Conclusion The key points for managing the multiple pregnancies, especially larger than 3 babies were the following ⑴All pregnant women had a good prenatal care. ⑵Pregnant women rested well, anemia must be corrected, and the adequate nutrition was supplied, especially in third trimester. ⑶Using ultrasound to determine the cervix length and evaluate the risk of preterm delivery, when the preterm delivery sign happened, the inhibiting drugs must be used cautiously and rationally. ⑷ After the antenatal dexamethasone therapy was applied around 36 gestation weeks, selective cesarean section was preferred to deliver the babies. If good prenatal care can be applied, a good outcome for triplets and quaternion can be achieved, close to that of same gestation weeks singleton.

Objective To investigate influence of ureaplasma urealyticum(UU)infection on low genital tract during the pregnancy.The ureaplasma urealyticum was detected by the polymerase chain reaction(PCR) in women vaginal discharge. The patients were divided into infection group and control group.Results 624(25 68%) in 2430 cases of vaginal discharge had ureaplasma urealyticum infection. Infection rates of ureaplasma urealyticum was 24 53% in second trimester and 28 86% in third trimester respectively.184 cases (32 05%) of the premature rupture of membranes in infected group were significantly higher than 20 cases(22%) in control group. The UU infection incidence in small for gestation(SGA) age was increased in the infection group (45/574 vs 2/100)P

Objective To measure the blood levels of mercury and arsenic of mother and umbilical cord, and analyze the relationship between these levels and outcome of pregnancy and complications. To explore the influential factors in order to guide a good birth and good care in pregnancy and lying-in women.Methods A total of 400 cases who were hospitalized in Xinhua hospital from March 2009 to August 2009 were included in this study. The blood levels of arsenic of mother and umbilical cord were determined by microwave digestion method and inductively coupled plasma emission mass spectroscopy method ( ICP-MS),while mercury determination was made using flame atomic absorption spectrophotometer (AAS). In addition, pregnant women were required to fill the questionnaire including their living environments, life styles,diet habits and etc. Results The newborn cord blood levels of mercury and arsenic were positively related to those of their mothers( r =0.88,0.91 respectively, P ＜0.05). The adverse pregnancy rates and complications rates in blood elevated mercury and arsenic groups were significantly higher than in the normal groups( x2 =7.07, 9. 94 respectively, P ＜ 0.01 ). Multiple regression analysis showed that the newborn blood mercury levels were significantly correlated to sea-fish eating, fruits supplement and ventilation during pregnancy. The former was a risk factor( Wald χ2 =8.28, P ＜0.01 ) and the later two were protective factors( Wald χ2 = 9.02, 6.05 respectively, P ＜ 0.05 ). Multiple regression analysis also showed that the newborn blood arsenic levels were significantly correlated to eating canned food, using cosmetics and education degree. Eating canned food and using cosmetics were risk factors(Wald χ2 =7.35, 10.38 respectively,P ＜ 0.05 ) while high education degree was protective factor( Wald χ2 = 8.87, P ＜ 0.05 ). Conclusion The newborn cord blood levels of mercury and arsenic were related to those of their mothers, environmental factors and health care during pregnancy. Health education and care during pregnancy were effective approaches for preventing mercury and arsenic poisoning.

Objective To investigate the correlation between KLF14 gene rs4731702 locus polymorphism and gestational diabe-tes mellitus (GDM) and relation between its different genotypes with BMI and insulin resistance.Methods This study adopted the case-control method.One hundred pregnant women of GDM (GDM group) and one hundred healthy pregnant women (normal con-trol group) were randomly selected as the research subjects and performed the physical examination ,biochemical indicators detec-tion.HOMA-IR and HOMA-β were calculated.The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was adopted.The genotyping of KLF14 gene rs4731702 locus in the two groups was performed.The genotypes and allele gene frequency were compared between the two groups and the GDM association analysis was conducted.Results The C alleles fre-quency and CC genotype frequency of KLF14 gene rs4731702 locus in the GDM group was significantly higher than that in the con-trol group ,the difference was statistically significant (P< 0.05).The patients with genotype CC in the GDM group had higher BMI ,FPG ,TG ,HbA1c and HOMA-IR as compared with those carrying genotype CT and TT ,the difference was statistically signif-icant(P<0.05).Also they had lower FINS ,HDL and HOMA-βas compared with carriers of genotype CT and TT ,the difference was statistically significant(P<0.05).The multivariate analysis showed that the genotype CC of KLF14 gene rs4731702 locus was closely related with GDM (P=0.005 ,RR=25.128).Conclusion The genotype CC of KLF14 gene rs4731702 locus plays a role in the pathogenesis process of GDM ,may be susceptibility genes for GDM ,which is also related to the abnormal lipid metabolism ,islet dysfunction and obesity.The polymorphism study of KLF14 gene rs4731702 locus helps to reveal the relation between lipid metabo-lism abnormality and insulin resistance with GDM onset.

Objective To explore the prenatal diagnosis and factors influencing prognosis of congenital diaphragmatic hernia (CDH).Methods Fifty seven cases of CDH prenatally diagnosed by ultrasound in the Department of Obstetrics,Xinhua Hospital from January 1,2006 to March 31,2013 were retrospectively reviewed.Prognosis and risk factors were analyzed by ultrasound characteristics and newborn status.Statistical analysis was performed using t test,x2 test and Logistic regression.Results Forty-five (79％)of all cases were left-side CDH and the rest 12 (21％)were right-side.Fourteen fetuses were aborted.Thirty three were delivered and treated surgically,twenty-three infants survived after surgery and ten died,with an overall survival rate of 40％ (23/57).The other ten cases were delivered,but died of no treated surgically.The birth weight of the surviving neonates was heavier than that of those who died [(3 173 ±348) vs (2 846±568)g,t=2.238,P=0.033].The gcstational age at diagnosis of the aborted fetuses was younger than that of the surviving neonates [(24.0 ± 4.1) vs (30.0± 6.0) weeks,t=3.181,P=0.003].Twenty-one (91％,21/23) of the surviving neonates had a lung-to-head ratio (LHR) ≥ 1.4.A linear trend test showed a negative correlation between LHR and adverse outcome (x2=9.223,P=0.002) with a correlation coefficient-0.730 (P=0.000).LHR＜1.4 (OR=35.867,95％CI:1.861-691.341,P=0.018) and polyhydramnios (OR=9.285,95％CI:1.102-78.212,P=0.040) were risk factors for neonatal death.Right-side CDH (OR=0.158,95％CI:0.009-2.824,P=0.210),liver herniation into the thorax (OR=1.730,95％CI:0.144 20.707,P=0.665) and birth weight (OR=l.002,95％CI:0.999-1.005,P=0.135) were not risk factors.Conclusions The survival rate of CDH is still low.Gestational age on diagnosis,presence of polyhydramnios and LHR＜ 1.4 predict an adverse outcome.

Objective To determine the perinatal outcomes and its risk factors of fetuses prenatally diagnosed with the double bubble sign on ultrasound scanning.Methods The clinical data of 57 cases of fetal double bubble sign which was prenatally diagnosed by ultrasound in Xin Hua Hospital from January 1,2000 to December 31,2013 were retrospectively analyzed.The neonates survived not less than 42 days were as surviving group,and who survived lower than 42 days were as dead group.The t test,x2 test and Logistic regression were used for statistical analysis of the data.Results Of 57 fetuses with the double bubble sign,the incidence of polyhydramnios,intrauterine fetal death and associated anomalies was 88％ (50/57),4％ (2/57) and 23％ (13/57),respectively.Induced labor to terminate the pregnancy was occurred in eight cases,the other 49 cases had live births but ten neonates abandoned therapy.Thirty-nine live babies accepted surgical treatment with one dead,and the other 38 neonates survived not less than 42 days.The overall 42-day survival rate was 67％ (38/57) and the surgical survival rate was 97％ (38/39).Compared with surviving neonates,the neonates who died had lower gestational age [(35.8±3.0) vs (38.1 ± 1.9) weeks,t=2.859,P＜0.01] and birth weight [(2 229±567) vs (2 830±558) g,t=3.136,P＜0.01],and a higher incidence of prenatally diagnosed structural anomalies [3/11 vs 3％ (1/38),P＜0.05] and karyotype anomalies detected after birth [3/11 vs 3％ (1/38),P＜0.05].Logistic regression analysis showed that the fetuses with the double bubble sign and fetal growth restriction had a higher risk of complications (OR=9.893,95％CI:1.758 55.661,P=0.009).Conclusions Fetuses with double bubble sign have adverse outcome if they complicating preterm delivery,low birth weight,prenatally diagnosed structural anomalies and karyotype anomalies.