The acupuncture manipulation is an important factor for achieving a good therapeutic effect in acupuncture practice.The authors review recent studies on acupuncture manipulation and its qualification from a) differences of the therapeutic effects of acupuncture manipulation,b) quantification of acupuncture manipulations,and c) instruments for delivering and sampling the analogued parameters of different acupuncture manipulations,computer analysis software,etc.Most studies revealed that different acupuncture manipulations had different clinical efficacies.Meanwhile,researches on the quantification of acupuncture manipulations and some related instruments also have made a bigger progress.Nevertheless,there still exist many problems,such as difficulties of various acupuncture techniques in unification in clinic,stronger personal subjectivity in performing manipulations,etc.We should overcome these problems and conduct more researches,so as to raise the clinical curative effect further.

Objective To observe the therapeutic effect of oral use of Yibai Tongfeng Pills for the treatment ofgouty arthritis with damp-heat obstruction. Methods One hundred and sixteen gouty arthritis patients were randomized into treatment group (N = 62) and control group (N = 54). The treatment group was treated with Yibai Tongfeng Pills orally, and the control group was treated with oral use of Colchicine Tablets and Sodium Bicarbonate Tablets at acute stage and with oral use of Benzbromarone Tablets at remission stage. Twelve weeks constituted one treatment course. Clinical efficacy of the two groups was evaluated after treatment, and the changes of C-reactive protein(CRP), white blood cell(WBC) and uric acid(UA) levels in the blood of the two groups were observed before and after treatment. Results (1) The total effective rate of the treatment group was 93.55%and that of the control group was 83.33%, the difference being significant(P<0.05).(2) After treatment, the scores of syndrome manifestations of the two groups were obviously decreased(P < 0.05 compared with those before treatment), and the decrease in the treatment group was superior to that in the control group(P<0.05) . (3) After treatment, CRP, WBC and UA levels in the blood of the two groups were much improved(P < 0.05 compared with those before treatment), and the improvement of CRP in the treatment group was superior to that in the control group(P<0.05), while the differences of the improvement of WBC and UA between the two groups were insignificant (P > 0.05). Conclusion Yibai Tongfeng Pills are effective for the treatment of gouty arthritis with damp-heat obstruction by improving syndrome manifestations and rapidly decreasing CRP level.

Objective To observe the antioxidative dosage-effect relationship of Marigold lutein, and provide experimental data for clinical use. Methods The mice were randomly divided into seven groups:blank control group, model control group, 1 mg/kg lutein group, 5 mg/kg lutein group, 25 mg/kg lutein group, 125 mg/kg lutein group and 625 mg/kg lutein group. The mice in blank control group were dealt with saline solution by intraperitoneal injection, the others were dealt with D-galactose (120 mg/kg) by intraperitoneal injection for seven weeks to make oxidative damage model, meanwhile the mice were given corresponding dose of the drug. Subsequently, the level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in the serum were measured, and the antioxidative dosage-effect relationship was observed. Results The 1, 5, 25 mg/kg lutein reduced the MDA level and increased SOD activity, and the 125, 625 mg/kg dose of lutein did not show significant antioxidant activity. Conclusion Lutein has significant antioxidant activity in mouse dealt with D-galactose within the dose range of 1-25 mg/kg. The results suggest that the clinical dosage range of lutein should be kept within reasonable limits.

The aim of this study is to explore the tissue distribution of PEGylated puerarin in acute myocardial ischemia model rats. Healthy male SD rats were randomly divided into two groups (30 each). Both were given PEGylated puerarin at a dose of 488 mg x kg(-1). After 5 min of medication, one group was normal rats, another group with acute myocardial ischemia was established by peritoneal injection of 50 mg x kg(-1) isoprenaline. After administration, the animals were executed at 30, 60, 90, 120, 150 and 180 min, then heart, liver, spleen, lung, kidney were extracted. The content of puerarin in organ tissue was determined by HPLC. The results showed that the AUC of tissue distribution of PEGylated puerarin in normal rats was liver > kidney > heart ≈ spleen > lung > brain. While the AUC of tissue distribution of PEGylated puerarin in acute myocardial ischemia model rats was liver ≈ heart > kidney > lung ≈ spleen > brain. AUC(heart) of PEGylated puerarin in acute myocardial ischemia model rats was 1.7 times than that of the normal rats, and there was significant difference (P < 0.05). Thus, PEGylated puerarin had a good heart-targeting property in early myocardial infarction area, drugs could accumulate in the ischemic myocardium. It provided important information for further study and clinic use of PEGylated puerarin.

ObjectiveTo investigate the effectiveness of combined oxaliplatin regimen as adjuvant chemotherapy for hepatocellular carcinoma and to evaluate the efficacy of using adenosine triphosphate tumor chemosensitivity assay (ATP-TCA) for direction of individual chemotherapy.MethodsThe twenty-six patients with primary hepatocellular carcinoma were operated.Specimens were collected and adenosine triphosphate tumor chemosensitivity assay (ATP-TCA) was applied to evaluate the sensitiveness of chemotherapy agent(Adriamycin, Mitomycin, Mitoxantrone, Oxaliplatin, Irinotecan, 5-FU, Gemzar, Carboplatin, Cisplatin, Docetaxel and Etoposide).Sensitive group (SG) was from from 11 patients who were sensitive to oxaliplatin, and control group was from the other 16 patients who were not sensitive to oxaliplatin.All the twenty-six patients received oxaliplatin combined with 5-FU or capecitabine regimen chemotherapy.The effectiveness (CR,PR,SD,PD,ORR,OS and DFS) of the regimen according to RECIST criteria and WHO criteria for anticancer drugs toxicity and efficacy of ATP-TCA were evaluated.ResultsTwenty-six patients were successfully evaluated.In SG, six patients obtained complete remission(CR), three got partial remission(PR), one got stable disease (SD) and one patient got progression disease (PD).While in control group,four patients obtained CR,two patients got PR, five patients got SD and four got PD.No significant differences were found in overall survival (OS, P = 0.1116) and disease-free survival (DFS, P = 0.2328)between sensitive group and control group.But significant differences were found in overall response rate (ORR) (81.8％ vs 40.0％, P =0.0401) between two groups.Common toxicities were as follows:I to Ⅱdegree of myelosuppression was 53.8％, I to Ⅱ degree of gastrointestinal tract response was 50％, I to Ⅱ degree of liver function damage was 57.7％ and I to Ⅱ degree of neuropathy was 23.1％, respectively.Most of these toxicities were tolerable at grade 1 ～ 2.No significant differences were found in the toxicities between two groups.ConclusionsCombined oxaliplatin regimen might be an effective choice for adjuvant chemotherapy for HCC, which has with tolerable systemic toxicity.Application of ATP-TCA system might further improve the efficacy of this regimen by selecting right candidate.

Objective To investigate the expression of multidrug resistance protein such as multidrug resistance-associated protein 1 (MRP1),lung-resistance related protein (LRP), P-glycoprotein (Pgp),glutathione s-transferase (GST-π) and topoismerase Ⅱ (TOPO Ⅱ ) in hepatocellular carcinoma (HCC), which would be supplied for the clinical chemotherapy of HCC. Methods Twenty-six cases of HCC who underwent hepatectomy were enrolled and immunohistochemical (IHC) staining was carried out on all specimens for the detection of expression of MRP1,LRP,Pgp,GST-πand TOPO Ⅱ and the data was analyzed by image analysis system. Results The expression of five multidrug resistance protein in HCC tissue were significantly higher than those in adjacent tissue beyond cancer (P ＜0.05). The significant differences were found in the expression of Pgp,TOPO Ⅱ and GST-π between HCC tissue and distant metastasis (P ＜ 0.05 ). The expression of the five multidrug resistance protein in poorly differentiated HCC tissue was higher than that in well-differentiated tissue,while the significant difference was only found in the expression of TOPO Ⅱ (P ＜ 0.05 ). The significant association was not found between the expressions of five multidrug resistance protein in HCC tissue and the size of tumor,AFP, the portal vein tumor thrombus,hepatic cirrhosis and liver function. Conclusions Five multidrug resistance protein overexpression in various degrees in HCC tissue, which relates to some biological behavior of the cancer. Combined detection is of much benefit to the choice of the drug of chemotherapy and to the prediction of prognosis.

The primary objective was to develope a UPLC method for determine the concentration of buspirone hydroxychloride in plasma and to evaluate the effects of Aconiti Laterlis Radix and Aconiti Laterlis Radix compatibility of Glycyrrhizae Radix on CYP3A4 in vivo. ACQUITY UPLC BEH C18 column (2.1 mm x 10 mm, 1.7 microm) was used for the gradient elution with a 2.0 mmol x L(-1) ammonium acetate (pH 7.4, A)-acetonitrile (B) solution, 0-2.2 min, 10% - 60% B, 2.2-2.5 min, 60% B, 2.5-3.0 min, 60%-75% B, 3.0-3.5 min, 75% B, 3.5-4.0 min, 75%-10% B, at the flow rate of 0.3 mL x min(-1) at room temperature. The UV wavelenght was detected at 243 nm. The linear calibration curve ranged between 0.078 125-20.0 microg (r = 0.9975). The average recovery (n = 6) of buspirone hydroxychloride was 85.62% (RSD 6.8%). The results showed that this method has good specificity and repeatability, and which can be used for the determination of buspirone hydrochlorid in serum. In animial studies, single dose Aconiti Laterlis Radix extract treatment (0.5 g x kg(-1)) decreased buspirone hydroxychloride AUC(0-2 h) (52.8%, P = 0.020), increased CL/F (122%, P = 0.045). Compared to the saline treatment group, Aconiti Laterlis Radix compatibility of Glycyrrhizae Radix extract treatment has no effect on CYP3A4 in rat. The results indicated that Aconiti Laterlis Radix extract induced CYP3A4 while Aconiti Laterlis Radix compatibility of Glycyrrhizae Radix extract had no effect on CYP3A4 in vivo. Aconiti Laterlis Radix had been detoxified when be used as compatibility of Glycyrrhizae Radix.
Aconitum ; chemistry ; Animals ; Chromatography, High Pressure Liquid ; methods ; Cytochrome P-450 CYP3A ; Cytochrome P-450 Enzyme System ; genetics ; metabolism ; Drugs, Chinese Herbal ; administration & dosage ; Gene Expression ; drug effects ; Glycyrrhiza ; chemistry ; Herb-Drug Interactions ; Male ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo investigate the characteristics of embryonic toxicity of Senecio scandens, its total alkaloid and Qianbai Biyanpian so that to provide guidance for the safety of medication during pregnancy.

METHODTwo hundred and twenty pregnant SD rats were divided into 11 groups: control group, positive group (cyclophosphamide 10 mg x kg(-1)). Water extract of S. scandens (doses: 7.5, 15.0, 30.0 g herb of S. scandens per kilogram body weight respectively). Qianbai Biyanpian and total alkaloid at the same doses levels with the water extract of S. scandens (doses were expressed as herb of S. scandens per kilogram body weight). The test articles were given to the pregnant rats by gavage from day 6 to day 15 of pregnancy. Body weight and the food consumption of pregnancy rats, and fetal weight and length were measured. The number of absorbed and dead embryos was recorded. Fetuses were examined in viscus and bones.

RESULTWeight and the food consumption of pregnancy rats in high-dose of Qianbai Biyanpian and total alkaloids decreased. All treatment groups had no significant change in the number of absorbed embryos, but the stillbirths were significantly increased in high-dose groups of water extract and total alkaloids as compared with control group. Bone deformities such as fontanel expanding, hypoplasia of parietal bone, occipital bone and cervical arch were observed. Rib abnormality could also be seen in some rats. All water extract of S. scandens, Qianbai Biyanpian and total alkaloid could cause the bone abnormalities, but the percentage of bone deformities of total alkaloids was the highest (up to 80%).

CONCLUSIONS. scandens and its total alkaloids, its formula Qianbai Biyanpian can cause rat skeletal deformities in fetuses when they were given during pregnancy. It is suggested that S. scandens and the product containing S. scandens should not be used during pregnancy.

Alkaloids ; chemistry ; toxicity ; Animals ; Body Weight ; drug effects ; Drugs, Chinese Herbal ; toxicity ; Eating ; drug effects ; Embryo, Mammalian ; drug effects ; Female ; Male ; Pregnancy ; Rats ; Rats, Wistar ; Senecio ; chemistry

Objective:To investigate the level of serum adiponectin (APN), high mobility group box 1 (HMGB1) and insulin resistance in patients with acute cerebral infarction complicated by myocardial infarction, and try to investigate the correlation between them.Methods:From January 2016 to June 2019, 448 patients who diagnosed as acute cerebral infarction in Haiyang People's Hospital were selected.The patients were divided into myocardial infarction(MI) group (36 cases) and non-MI group (412 cases) based on whether they complicated with MI.And 50 healthy people were selected as healthy control group.Fasting venous blood was collected from all subjects, and the adiponectin, HMGB1, coagulation indicators, inflammatory indicators, myocardial infarction markers and insulin resistance were measured.Results:The markers of myocardial infarction (CK-MB, cTnⅠ, Mb), coagulation indicators (APTT, PT, AT-Ⅲ, ACT), inflammatory levels (HMGB1, APN, CRP, IL-6)and insulin resistance related indicators (FPG, FINS, HOMA-IR, ISI)in patients with MI were different from patients with non-MI [CK-MB: (25.33±4.61)μg/L vs.(21.85±4.73)μg/L, t=6.028, P<0.001; cTnⅠ: (7.96±0.98)μg/L vs.(4.89±1.05)μg/L, t=24.135, P<0.001; Mb: (91.07±15.21)g/L vs.(147.53±16.04)g/L, t=28.981, P<0.001; APTT: (34.02±6.12)s vs.(37.21±6.31)s, t=4.144, P=0.005; PT: (14.32±1.21)s vs.(12.94±1.37)s, t=8.390, P<0.001; AT-Ⅲ: (144.62±18.35)s vs.(167.53±20.04)s, t=9.382, P<0.001; ACT: (135.84±15.21)s vs.(145.06±16.02)s, t=4.711, P<0.001; HMGB1: (25.61±3.84)μg/mL vs.(19.27±4.21)μg/mL, t=12.456, P<0.001; APN: (6.03±0.78)mg/L vs.(9.16±0.97)mg/L, t=26.995, P<0.001; CRP: (46.12±2.87)mg/L vs.(39.36±3.21)mg/L, t=17.608, P<0.001; IL-6: (8.76±1.42)mg/L vs.(5.04±1.22)mg/L, t=25.238, P<0.001; FPG: (6.27±0.98)mmol/L vs.(5.62±1.05)mmol/L, t=5.106, P<0.001; FINS: (24.07±4.25)mIU/L vs.(15.84±4.46)mIU/L, t=15.235, P<0.001; HOMA-IR: (6.68±0.68)vs.(3.96±0.84), t=27.217, P<0.001; ISI: (-5.03±0.84)vs.(-4.57±0.97), t=3.963, P<0.001] and the healthy controls [CK-MB: (25.33±4.61)μg/L vs.(20.04±4.52)μg/L, t=7.280, P<0.001; cTnⅠ: (7.96±0.98)μg/L vs.(4.04±0.95)μg/L, t=24.482, P<0.001; Mb: (91.07±15.21)g/L vs.(194.23±14.79)g/L, t=42.067, P<0.001; APTT: (34.02±6.12)s vs.(40.89±6.02)s, t=7.090, P<0.001; PT: (14.32±1.21)s vs.(10.94±1.15)s, t=16.326, P<0.001; AT-Ⅲ: (144.62±18.35)s vs.(225.31±19.64)s, t=26.249, P<0.001; ACT: (135.84±15.21)s vs.(161.32±15.77)s, t=10.342, P<0.001; HMGB1: (25.61±3.84)μg/mL vs.(6.72±3.78)μg/mL, t=29.484, P<0.001; APN: (6.03±0.78)mg/L vs.(12.54±0.82)mg/L, t=44.604, P<0.001; CRP: (46.12±2.87)mg/L vs.(7.64±2.52)mg/L, t=79.626, P<0.001; IL-6: (8.76±1.42)mg/L vs.(2.22±1.29)mg/L, t=35.249, P<0.001; FPG: (6.27±0.98)mmol/L vs.(5.15±0.96)mmol/L, t=6.989, P<0.001; FINS: (24.07±4.25)mIU/L vs.(10.64±3.96)mIU/L, t=19.751, P<0.001; HOMA-IR: (6.68±0.68)vs.(2.44±0.66), t=33.705, P<0.001; ISI: (-5.03±0.84)vs.(-3.94±0.79), t=7.460, P<0.001]. The incidence of acute cerebral infarction complicated with myocardial infarction was negatively correlated with APN and AT-Ⅲ(APN: r=-0.405, P=0.001; AT-Ⅲ: r=-0.554, P<0.001), and positively correlated with HMGB1 and HOMA-IR(HMGB1: r=0.624, P=0.005; HOMA-IR: r=0.667, P<0.001). Conclusion:There is a significant negative correlation between the occurrence of acute cerebral infarction complicated with MI and the level of APN, and positive correlations between HMGB1 and insulin resistance.