Child ; Gastroesophageal Reflux ; diagnosis ; therapy ; Humans

Objective To investigate the anti-tumor effect and mechanism of Plumbagin against human large cell lung cancer cell line NL9980.Methods Plumbagin in different concentrations were set up to administrate NL9980 cell line.MTT test was performed to detect the inhibiting effects against tumor proliferation and IC50 concentration was identified.The influence of tumor apoptosis was observed by flow cytometry and the inhibiting effect against invasive ability was detected by Boyden chamber test.IC50 group and control group were set up and tumor cells were harvested 6 h,24 h and 48 h after administration.Realtime PCR was used to detect mRNA expression changes of bcl-2,bax,VEGF and CYCD1.Results MTT test showed obvious inhibiting effects of proliferation in NL9980 cell line and IC50 was 7.5 μmol/L.Plumbagin induced the apoptosis of tumor cells and showed a significant anti-invasive effect.Mean membrane-spanning cells were 161.59±47.32 and 26.58±9.07 in control group and IC50 group correspondingly.Down-regulations of bcl-2,VEGF and CYCD1 were detected and expression of bax gene increased.The effect of Plumbagin showed obvious time dependance (P ＜0.05).Conclusion Plumbagin has displayed the powerful antitumor effects against NL9980 cell line and it might work via multiple pathways.Plumbagin exhibits the prospect of being an effective drug against large cell lung cancer.

As a novel research tool in the field of the spinal biomechanics, the finite element method (FEM) complements and verifies the traditional research methods, which has been attached increasingly greater importance. In this paper, we briefly introduce the achievement made in the field of spinal biomechanics by FEM in the past decade, summarize the physiological and pathological behaviors of various spinal anatomical parts described by FEM, and present the basic view of using FEM to optimize the surgery instrument design.

The role of bracing for adolescent idiopathic scoliosis,which came into application in 1960's,has long been controversial.There are various brace instrument with different reported effectiveness.In this paper,we summarize the research findings and main views in the effectiveness of bracing for adolescent idiopathic scoliosis in the past ten years,and explore the possible causes leading to the different results.Besides,we are trying to point out the research trend,in hope of drawing a scientific conclusion on the effectiveness of bracing treatment in the near future.

Object To develop a new process for the extraction of camptothecin from leaves of Camptotheca acuminata Decne Methods By uniform experimental design after proper choice of solvent Results The optimal conditions were extraction with 16 times of 0 3% NaOH for three hours each time Conclusion This is the first attempt to use dilute NaOH solution for the extraction of camptothecin from the previously seldom utilized leaves of C acuminata, which resulted in a yield well over 0 1% The process resulted in a lower production cost and require less fire protection measures as compaired with the conventional use of alcohol

In order to supply the multichannel electrical stimulator, a DC-DC flyback switch mode power supply based on a high-performance current mode controller UC3845 has been designed. The experimental results indicate that the power supply has satisfied the demands and can supply the multichannel electrical stimulator and similar apparatuses.
Electric Power Supplies ; Electric Stimulation ; instrumentation ; methods ; Equipment Design

ObjectiveTo investigate the clinical significance of peripheral blood lymphocyte subsets in patients with idiopathic inflammatory myopathy (ⅡM).MethodsPeripheral blood lymphocyte subsets was determined by flow cytometry in 89 patients with polymyositis(PM ) or dermatomyositis ( DM ).The association between clinical features and peripheral blood lymphocyte subsets was analyzed by F test,t test and x2 test.ResultsPatients with active DM showed significant decreases in counts of CD3+ cell,CD3+CD4+ cell and CD3+CD8+ cell[(8±4),(5.4±2.8) and (2.6±1.6) ×108/L respectively],compared with those in inactive DM [(16±6), (10.4±5.6) and (5.6±3.8) ×108/L respectively] and healthy controls [(14±4), (8.3±2.8) and (4.6±1.7) ×108/Lrespectively](F=12.901,8.257,7.084; P＜0.05).Logistic regression analysis indicated that myositis disease activity could influence the counts of peripheral blood CD3+ cell,CD3+CD4+ cell and CD3+ CD8+ cell (b=0.211,0.344,0.289; P＜0.05 ).ILD in ⅡM could influence the counts of CD3+ cell and the ratio of CD3+CD4+cell (b=0.928,1.974; P＜0.05 ).Logistic regression analysis indicated that the count of CD3+CD8+ cell was risk factor for death,and the relative risk was 0.989(b=-0.011 ; P＜0.05).ConclusionPeripheral blood lymphocyte subsets may be regarded as useful laboratory parameters for monitoring RA disease activity.Decreased CD8+ T cell may predict a poor outcome of patients with IIM.

An alternative method based on an off-line solid phase extraction ( SPE ) combined with programmable temperature vaporizer-based ( PTV) large volume injection-gas chromatography-flame ionization detection ( LVI-GC-FID ) was developed. The goal of this study was to determine mineral oil saturated hydrocarbons ( MOSH ) in camellia seed oils. The purification condition of SPE columns with silver impregnated the activated silica gel and activated aluminum oxide was optimized. The optimal SPE cartridge was loaded with 10 g of Ag-activated silica gel per 10 g of activated aluminum oxide. The PTV initial temperature was set at 75℃ for 1 min (split 200:1), and heated from 75℃ to 370℃ at 250℃/min. Then the diverter valve was closed for 1 min and opened again with the split flow ratio changing to 50:1 . The injection volume was 40μL. The calibration curve of paraffin oil was liner in the range of 5-500 mg/kg with correlation coefficient of 0. 998. The detection limit (LOD) and the quantification limit (LOQ) of paraffin oils in hexane were 0. 26 mg/kg and 0. 80 mg/kg, respectively. The recoveries from spiked oil samples were between 93 . 3% and 112 . 7%, with relative standard deviation ( RSD ) of 1 . 8%-5 . 2%, the RSD of intra-day and inter-day were less than 2 . 6% . This procedure was applied to analyze the MOSH in 11 commercial camellia seed oils and the contamination was found to range from 6. 8 mg/kg to 76. 7 mg/kg. The method is simple in operation with high sensitivity, good reproducibility and low cost, and suitable for determination of MOSH in vegetable oils.

This thesis is about a new technology of program-controlled telemetry for pacemakers. The system utilizes digital logic circuit design, and the program-controlled part uses single chip to control for display and debug. PWM and reflectance telemetry may improve the preciseness and correctness of signal transmission, and reduce the power consumption of pacemakers and prolong the lifetime.
Electrocardiography ; instrumentation ; methods ; Equipment Design ; Humans ; Microcomputers ; Monitoring, Ambulatory ; methods ; Pacemaker, Artificial ; Signal Processing, Computer-Assisted ; Telemetry ; instrumentation ; methods

Objective:To assess the applicability of the symptom inventory scale of DSM-Ⅳ ADHD in 112 Chinese children.Methods:Samples from out patients were diagnosed with the scale and CCMD-3.Results:The tester-consistency reliability Kappa value of the symptom inventory scale is 0.89,P