1.Preliminary study of liver injury in patients with influenza A (H1N1).
Da-ming ZHOU ; Ji-jun JIANG ; Wen-hong ZONG ; Lei SHEN ; Zu-xuan HUANG ; Yun ZHANG ; Yong-feng YANG ; Xiao-feng HE ; Xin-gong ZHU
Chinese Journal of Hepatology 2010;18(12):940-941
Adolescent
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Adult
;
Aged
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Child
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Female
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Humans
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Influenza A Virus, H1N1 Subtype
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Influenza, Human
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complications
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pathology
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physiopathology
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Liver
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pathology
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physiopathology
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Male
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Middle Aged
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Retrospective Studies
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Young Adult
2.MicroRNA-218 promotes osteosarcoma cell apoptosis by down-regulating oncogene B lymphoma mouse Moloney leukemia virus insertion region 1.
Gui-Hua LAI ; Ai-Lan HUANG ; Zhi ZHAO ; Xing-Hao LU ; Wen-Xuan ZU
Journal of Southern Medical University 2018;38(5):505-510
OBJECTIVETo investigate the tumor-suppressing effect of microRNA-218 (miR-218) in osteosarcoma (OS) and explore its molecular mechanism.
METHODSWe examined the expression levels of miR-218 in 68 pairs of OS and adjacent tissue samples using qRT-PCR. Cultured human OS cell line Saos-2 was transfected with miR-218 mimics or anti-miR-218 mimics, and the cell apoptosis was assessed using CCK-8 assay, annexin V-FITC staining and Western blotting. We also analyzed the potential functional targets of miR-218 in Saos-2 cells using luciferase assay, qRT-PCR and Western blotting.
RESULTSThe expression level of miR-218 was lowered by at least 8 folds in OS tissues as compared with the adjacent tissues. In cultured Saos-2 cells, transfection with miR-218 mimics for 24, 36, and 48 h resulted in a significant reduction in the cell viability, while transfection with anti-miR-218 mimics significantly increased the cell viability. The cells transfected with miR-218 mimics showed an obviously enhanced expression of cleaved poly(ADP-ribose) polymerase (C-PARP) as compared with the cells transfected with anti-miR-218 mimics and the control cells. Flow cytometry demonstrated obviously increased apoptosis of the cells following miR-218 mimics transfection. We identified the oncogene B lymphoma mouse Moloney leukemia virus insertion region 1 (BMI-1) as a specific target of miR-218 in Saos-2 cells. BMI-1 expressions at both the mRNA and protein levels were significantly reduced in Saos-2 cells overexpressing miR-218 but increased in the cells with miR-218 knockdown as compared to the control cells. Luciferase reporter assay indicated that miR-218 directly inhibited the expression of BMI-1 via binding to its 3'-UTR in OS cells.
CONCLUSIONmiR-218 can promote OS cell apoptosis and plays the role as a tumor suppressor by down-regulating BMI-1.