1.Determination of total organic acids and salicylic acid in extract of Radix isatidis.
Li MA ; Jian-yuan TANG ; Zu-lun LU ; Qing-wen LIAO ; Xiao-he XIAO ; Xiang-jun ZHAO ; Cheng JIN
China Journal of Chinese Materia Medica 2006;31(10):804-806
OBJECTIVETo develop a quantitative method for determination of the total organic acids and salicyclic acid in the extract of Radix Isatidis.
METHODThe total organic acids were determined by acid-base titration and the salicylic acid was determined by HPLC.
RESULTIt was shown that contents of total organic acids and salicylic acid in the extract of Radix Isatidis were 13.0% and 0.22%, respectively.
CONCLUSIONThe method can control the quality of this extract effectively and accurately.
Carboxylic Acids ; analysis ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Isatis ; chemistry ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Salicylic Acid ; analysis ; Technology, Pharmaceutical
2.An outbreak of human Streptococcus suis serotype 2 infections presenting with toxic shock syndrome in Sichuan, China.
Wei-zhong YANG ; Hong-jie YU ; Huai-qi JING ; Jian-guo XU ; Zhi-hai CHEN ; Xiao-ping ZHU ; Hua WANG ; Xue-cCheng LIU ; Shi-wen WANG ; Lun-guang LIU ; Rong-qiang ZU ; Long-ze LUO ; Ni-juan XIANG ; Hong-lu LIU ; Wen-jun ZHONG ; Li LIU ; Ling MENG ; Heng YUAN ; Yong-jun GAO ; Hua-mao DU ; Yang-bin OU ; Chang-yun YE ; Dong JIN ; Qiang LV ; Zhi-gang CUI ; Yan HUANG ; Shou-yin ZHANG ; Xiang-dong AN ; Ting HUANG ; Xing-yu ZHOU ; Liao FENG ; Qi-di PANG ; Yue-long SHU ; Yu WANG
Chinese Journal of Epidemiology 2006;27(3):185-191
OBJECTIVEIn mid-July 2005, five patients presented with septic shock to a hospital in Ziyang city in Sichuan, China, to identify the etiology of the unknown reason disease, an epidemiological, clinical, and laboratory study were conducted.
METHODSAn enhanced surveillance program were established in Sichuan, the following activities were introduced: active case finding in Sichuan of (a) laboratory diagnosed Streptococcus suis infection and (b) clinically diagnosed probable cases with exposure history; supplemented by (c) monitoring reports on meningococcal meningitis. Streptococcus suis serotype 2 infection was confirmed by culture and biochemical reactions, followed by sequencing for specific genes for serotype and virulence factors.
RESULTSFrom June 10 to August 21, 2005, 68 laboratory confirmed cases of human Streptococcus suis infections were reported. All were villagers who gave a history of direct exposure to deceased or sick pigs in their backyards where slaughtering was performed. Twenty six (38%) presented with toxic shock syndrome of which 15 (58%) died. Other presentations were septicaemia or meningitis. All isolates were tested positive for genes for tuf, species-specific 16S rRNA, cps2J, mrp, ef and sly. There were 136 clinically diagnosed probable cases with similar exposure history but incomplete laboratory investigations.
CONCLUSIONAn outbreak of human Streptococcus suis serotype 2 infections occurred in villagers after direct exposure to deceased or sick pigs in Sichuan. Prohibition of slaughtering in backyards brought the outbreak to a halt. A virulent strain of the bacteria is speculated to be in circulation, and is responsible for the unusual presentation of toxic shock syndrome with high case fatality.
Animals ; Bacteremia ; epidemiology ; microbiology ; China ; epidemiology ; Disease Outbreaks ; Humans ; Meningitis, Bacterial ; epidemiology ; microbiology ; Shock, Septic ; epidemiology ; microbiology ; Streptococcal Infections ; epidemiology ; microbiology ; veterinary ; Streptococcus suis ; isolation & purification ; Swine ; Swine Diseases ; microbiology