1.Microwave Digestion Used in Soil Total Chromium Determination
Journal of Environment and Health 1992;0(02):-
Objective To develop a pretreatment method with the microwave digestion for the determination of soil total chromium. Methods The nitric acid and hydrofluoric acid were added into the samples, the microwave digestion under the certain condition was conducted and then the sulfuric acid was added on the controllable electric stove. Flame atom absorption spectrophotometry or diphenylcarbohydrazide spectrophotometry was used to determine soil total chromium according to the condition. Results RSDs were from 1.20% to 11.08%. The Environmental Soil Standard test demonstrated that the results were in the guarantee value, as for ESS-1, RSD=2.57%, as for ESS-3, RSD=2.20%. Conclusion The microwave digestion is considered as a good pretreatment method for the determination of soil total chromium with the advantages of safe, rapid and accurate.
2.Innate immune cell-derived IL-l7 mediating organ ischemia reperfusion injury
Zheng ZOU ; Yu PENG ; Dawei ZOU
International Journal of Pediatrics 2016;43(2):113-118
IL-17,as a pro-inflammatory cytokine,is one of the early initiation factors of the inflammato-ry response induced by T cells.It can induce and regulate multiple immune responses.Recent studies have re-vealed that myeloid neutrophils,macrophage,mast cell and other innate immune cells all can secrete large a-mount of early responding IL-17 in organs suffering ischemia/hypoxia,and in turn can activate,amplify and re-cruit neutrophils to the reperfusion-damaged tissue to release large amount of free radicals and lysozyme that cause IRI.Researchers have also provided evidence that appropriate administration of anti-IL-17 mono-antibody to neutralize IL-17 during early reperfusion stage would reduce the tissue damage.The purpose of this review is to summarize the research progress of the effects of IL-17 produced by innate immunocyte on organ reperfusion injury.
3.The relationship between mitochondrial DNA damage and retinal pigment epithelium cells
Yongzhen YU ; Xiulan ZOU ; Yuping ZOU
Tianjin Medical Journal 2015;(9):1079-1081
Mitochondrial DNA (mtDNA) is a genetic effect DNA molecule of double closed loop, and is crucial for cells and their functions. Mitochondria take an active part in physiological activities of retinal pigment epithelium (RPE) cells. The oxidative stress is usually occurred in RPE for its active metabolism, which can lead to mitochondria and mtDNA dam?age. Once mitochondria and mtDNA lesions have not been repaired timely, the lesions can be accumulated, which can cause dysfunctions and damaged-structures of RPE and mitochondria, and can motivate the progression of cell apoptosis. In the end it can result in some ocular related diseases such as aged-related macular degeneration (AMD). This study reviewed the functional relationship between mtDNA and RPE, and repair and detection methods of mtDNA damage.
4.Application of pencil beam model based on point kernel in intensity modified radiation therapy.
Journal of Biomedical Engineering 2014;31(1):166-171
In this paper, a method for dose calculation with pencil beam kernels constructed by point kernel superposition was proposed to accelerate the dose calculation during intensity optimization iteration. With this method, the direct aperture optimization method can be integrated in the planning system based on point kernel convolution/superposition model. The dose calculation time was also reduced during the iteration. From the result of the phantom and clinical patient data test, it was concluded that this method could be used for the intensity optimization of iteration dose calculation as the satisfied precision due to the optimization result coherence obtained. By implementing the method in the planning system product based on point kernel convolution/superposition model, a lot of additional research and development works for the pencil beam dose calculation model as well as the product maintenance cost can be avoided.
Algorithms
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Humans
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Models, Theoretical
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Phantoms, Imaging
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Radiotherapy Dosage
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Radiotherapy Planning, Computer-Assisted
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Software
5.Application of Intravenous Administration of Anisodaminal Hydrochloridum in Gastric Ultrasonic Diagnosis
Chinese Journal of Ultrasonography 1992;0(00):-
With the method of fluid-filled stomach and intravenous administration of anisodaminal hydrochloridum(654-2), 94 cases of 11 gastroduodenal diseases including gastric cancer and others were examined with ultrasound. Comparison of the ultrasonography with the pathological changes was done. The finding rate of gastric foci which equal to or more than 5mm in diameter was 96.8%. The correct rate of diagnosis was 80.9%. The accuracy in differentiating early gastric cancer from advancing gastric cancer was 87.5%. The correct rate for judging invasive depth of stomach wall by gastric cancer was 82.9%. It can also show the distinct ultrasonic features for gastric submucosal diseases.
6.THE STUDY ON TRANSPLANTATION OF THREE-DIMENTIONAL CULTURED ASMC-COLLAGEN GEL TO SUBCUTANEOUS
Acta Anatomica Sinica 1953;0(01):-
Objective To investigate if aortic smooth muscle cells (ASMCs) cultured three-dimentionally with collagen gel can still produce elastic fibers and the acceptance of hosts after transplantated in subcutaneous of rats. Methods AMSC-collagen gels that had been cultured for 2 weeks by three-dimentionally imitating the dermis substitute were transplanted to rats subscutaneous. After 4,7,10,14 and 28 days, the transplantations were examined via HE staining, Gomori aldehyde fuchsin staining, ?-actin immunohistochemistry and elastin immunohistochemistry. Results The amount of elastic fibers increased significantly in the week, whereas on the 28th day, no elastic fibers were detected.There was no remarkable leucocyte infiltration around the ASMC-collagen gel during 28 days.Conclusion Further studies are needed to explore the ASMC-collagen gels as a kind of elastic dermis substitute.
7.AORTIC SMOOTH MUSCLE CELLS PRODUCED ELASTIC FIBERS IN THREE-DIMENSIONAL CULTURE
Acta Anatomica Sinica 1953;0(01):-
Objective To Study if aortic smooth muscle cells(ASMC) can produce elastic fibers in three-dimensional culture imitating the dermis substitute. Methods Primary culture of ASMCs were obtained by explanting of thoracic aortae from one-week old SD rats. ?-actin immunocytochemistry was used to approve the smooth muscle cells. After subculture, ASMCs were mixed with gels composed of collagen, chitin and glucosaminoglycans, then, three-dimensional culture was carried out. After 1 week and 2 weeks, Gomori aldehyde fuchsin staining and elastin immunocytochemistry were applied to detect the elastic fibers in the ASMC-collagen gels. Results More than 98% of the cultured cells were approved as smooth muscle cells by ?-actin immunocytochemistry. After both 1 and 2 weeks of three-dimentional culture, elastic fibers were detected in the ASMC-collagen gels by both stainings.Conclusion ASMCs can synthesize elastin and produce elastic fiber in three-dimentional culture imitating the dermis substitute.
8.Analysis on pathogens isolated from lower respiratory tract sputum samples of tracheotomy patients and nurses′hands surface samples
International Journal of Laboratory Medicine 2015;(17):2521-2523
Objective To investigate the distribution of pathogens isolated from lower respiratory tract sputum samples of trach‐eotomy patients who suffered from severe craniocerebral injury and nurses′hands surface samples ,and analyze the correlation be‐tween them .Methods Lower respiratory tract sputum samples of 97 tracheotomy patients suffered from severe craniocerebral inju‐ry and hands surface samples from nurses who just washed their hands after the sample collection were collected .Then the samples were sent to the microbiological lab for pathogen isolation and identification ,the results were statistically analyzed by using WHO‐NET5.4software.Results 388sampleswerecollectedaltogether,including194sputumsamplesand194nurses′handssurface samples .633 pathogens were isolated altogether ,including 452 strains of G- bacteria (71 .41% ) ,134 strains of G+ bacteria (21 .17% ) and 47 strains of fungi(7 .42% ) .The top five species of G- bacteria which took the largest proportion and caused the lower respiratory tract infection were Peudomonas aeruginosa(12 .16% ) ,Acinetobacter baumannii(9 .63% ) ,Klebsiella pneumoniae bacteria(7 .10% ) ,Stenotrophomonas maltophilia(5 .21% ) ,Escherichia coli(4 .89% );the primary species of G+ bacteria were coag‐ulase negative staphylococcus(6 .79% ) ,Staphylococcus aureus(2 .36% );the primary fungus was Monilia albicans(4 .24% ) .The top five G- bacteria species which took the largest proportion and isolated from hands surface samples were Pseudomonas aeruginosa (6 .79% ) ,Acinetobacter baumannii(5 .84% ) ,Escherichia coli(4 .91% ) ,Klebsiella pneumoniae(3 .94% ) ,Stenotrophomonas malto‐philia(3 .79% );the primary species of G+ bacteria were coagulase negative staphylococcus (9 .63% ) ,Staphylococcus aureus (2 .36% ) .The primary fungus was Monilia albicans(3 .00% ) .Conclusion Tracheotomy patients who suffered from severe cranio‐cerebral injury with lower respiratory infection are very possible to have cross‐infection ,sanitary management of nurses′hands asep‐tic manipulation procedures should be strengthened .
9.Progress in Fabry disease
International Journal of Pediatrics 2010;37(3):283-286
Fabry disease is an inherited metabolic disease, resulting from a deficiency of the enzyme a-galactosidase A activity. Principal clinical manifestation of Fabry disease was limb pain episodes, angioceratoma, and progressive damage of heart,brain, kidney. Although Fabry disease cannot be cured at present,early diagnosis and enzyme replacement therapy can change the prognosis completely.
10.Resources of pteridophyte plants in Zhejiang-Dongqian lake area
International Journal of Traditional Chinese Medicine 2011;33(7):609-611
Objective To make clear the resources of pteridophyte in Zhejiang-Dongqian lake area.Methods Field investigation, data reference and consultation to local peasants were adopted. Results There are 20 famines, 23 genera, and 26 species of medicinal ferns in Dongqian Lake area. Conclusion Medicinal ferns are abundant in Zhejiang-Dongqian lake area.