Objective To study and summarizes the methods ofthe standardized hospital infection manage -ment technology in order to prevent and control hospital infection scientifically .Methods Taking the chance of im-provingthe execution of the standardized hospital infection management issued by the Ministry of Health ,we realized organizational execution by enhancing the responsibility decomposition , mechanism implementation by standardized completion,ideological implementation by education and training ,the implementation of standards by inspection and monitoring and finally form a team by enhanced communication .Results The technicalstandards of hospital infection management was implemented and the quality of hospital infection control was improved progressively .Conclusion It is an effective way to guarantee medical security to improve the execution of the technical standard of hospital infec -tion management .

ObjectiveTo investigate the effects of moxibustion on the expression of tryptophan hydroxylase 2(TPH2) mRNA in median raphe nuclei and the activity of monoamine oxidase (MAO) in serum and cortex in depression model rats and to explore its mechanism.MethodsFemale SD rats were randomly divided into control group(n=6),model group(n=7) and moxibustion group(n=7).The rat depression model was induced by giving isolation-housing in combination with chronic unexpected mild stress(CUMS) for 21 days.The hemi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) was employed to detect the relative expression of TPH2 mRNA,colorimetry was used to test the activity of MAO.ResultsThe expression of TPH2 mRNA in median raphe nuclei in rats of the moxibustion group was higher than that in the model group( relative dentisty value:(0.3759 ± 0.0272)vs(0.2573 ± 0.0581 ),P ＜ 0.05 ).The activity of MAO in serum and cortex was reduced in rats of the moxibustion group( ( 1.919 ± 1.243 ) U/ml,( 1292.048 ± 90.072 ) U/mg protein,respectively) compared with that in the model group ( (4.117 ± 2.727 ) U/ml,( 1441.345 ± 117.050 ) U/mg protein,respectively).ConclusionMoxibustion has the effects of improving depression,increasing the expression of TPH2 mRNA in median raphe nuclei and reducing the activity of MAO in serum and cortex might be one of its mechanisms.

The reproductive toxicity of environmental estrogens(EEs)has become an extensively studied subject in environmental science and reproductive medicine nowadays.Many epidemiology data and experimental evidences indicated that EEs may significantly cause the disorders of male sex differentiation such as microphallus,hypospadia,cryptorchidism and masculinization behavior disorder and so on.The mechanism includes estrogenic activity,anti-androgenic activity as well as other feedback regulation and signal transmission which influence hormones metabolism.

Objective To investigate prevalence of resistance genes for β-lactams in clinically isolated multidrug-resistant Acinetobacter baumannii (MDRAB)in a hospital.Methods 22 clinically isolated MDRAB strains were performed antimicrobial susceptibility testing,resistance genes forβ-lactams (TEM,SHV,CTX-M,PER,DHA, IMP ,VIM ,SIM,OXA-23,OXA-24,and OXA-58)in these strains were detected with polymerase chain reaction. Results The resistant rates of 22 isolates of MDRAB to ceftazidime,cefotaxime,cefepime,gentamycin,amikacin, ciprofloxacin,and compound sulfamethoxazole were all 100.00%;to imipenem,meropenem,and cefoperazone/sul-bactam were 50.00%,40.91 %,and 31 .82% respectively,intermediated rates were 31 .82%,36.36%,and 31 .82% respectively.The carriage rates of OXA-23,TEM,IMP ,and PER were 100.00% (n =22),72.73 %(n=16),54.55% (n = 12),and 18.18% (n =4)respectively.Detection results of SHV,CTX-M,DHA,VIM , SIM,OXA-24,and OXA-58 were all negative.Conclusion Carriage of IMP ,TEM,PER,and OXA-23 resistance genes are the major resistance mechanisms of MDRAB to β-lactamase antimicrobial agents in this hospital.

Objective To investigate the effects of children's mentality on mental symptoms during recovery period of ketamine anesthesia. Methods According to the mentality, forty-five children undergoing smalminor surgery were assigned into active group, stable group and fretful group with 15 cases each. Child was anaesthetized by ketamine alone. RP, BP, HR and SpO_2 were recorded at the end of operation, 5,10 and 30 min after operation. Meanwhile the expression, feeling, behavior, talking and activity were observed. Results RR, BP, HR in active group and fretful group were significantly higher than those in stable group. There was a significant difference in the expression, feeling, behavior, talking and activity among three groups. Conclusion Children's mentality has a significant effect on mental symptoms and hemodynamics during recovery period of ketamine anesthesia.

Objective:To investigate the dynamic change of tetramethylpyrazine (TMP) in processing of Chuangxiongchatiao Granules. Methods: To explore the influences of the different extraction, concentration and drying method on the transferring rate of TMP by HPLC. Results: Transferring rate of TMP is higher by alcohol than by water. The loss of TMP in concentrating process is lower by rotating distillation than by direct heating concentration and by reduced pressure concentration. The transferring rate of TMP is higher by reduced pressure desiccation than by ordinary pressure. Conclusion: The better extrating way of Chuangxiong is by alcohol in the processing of Chuangxiongchatiao Granules; the concentrating and drying process should be done at the low temperature and by rapid dry process.

OBJECTIVE:To provide reference for clinical rational use of anti-epileptic drugs. METHODS:In this retrospective review,serum concentrations of anti-epileptic drugs in a total of 499 patients who were treated with anti-epileptic drugs (such as sodium phenytoin,phenobarbital,carbamazepine and valproate sodium) in our hospital during 2007 were analyzed statistically. RESULTS:Among the patients receiving one kind of anti-epileptic drugs,206 cases (61.49%) were within normal range in blood concentration versus only 45 cases (44.12%) for patients receiving combined drugs. In addition,the above four drugs (sodium phenytoin,phenobarbital,carbamazepine and valproate sodium) were detected in 59.68% of the patients who took Chinese medicines,and among them,3.23% were within normal range in blood concentration. CONCLUSION:Monitoring on serum concentrations of anti-epileptic drugs is conducive to a better control of therapeutic concentration. It is advisable to refrain from using anti-epileptic drugs in combination but to adopt individualized administration. In addition,great importance should be attached to whether there are chemo-synthetic drug components contained in Chinese medicine.

Objective To evaluate renal plication and nephropexy in the treatment of giant hydronephrosis. Methods 18 patients with giant hydronephrosis underwent relief of the obstruction as well as renal plication and nephropexy were reviewed. Results The patients have been followed up for three months to three years and their hydronephrosis improved a lot.The effected kidney showed some recovery of function on excretory urography. Conclusions Renal plication and nephropexy can promote recovery of renal anatomy and function after the relief of obstruction.

Objective To investigate the clinical application and value of spiral CT(SCT) in the diagnosis of renal cell carcinoma (RCC) with inferior vena cava (IVC) tumor thrombus. Methods Spiral CT scans were performed for 4 cases clinically confirmed as RCC with IVC tumor thrombus.The imaging findings were checked with post operative pathological findings. Results RCC with IVC tumor thrombus were assessed in all the 4 patients by SCT.The carcinoma stage and the type of the tumor thrombus were also determined.Operative findings have been the same as SCT. Conclusions SCT,a non traumatic and effective method,can make accurate diagnosis of RCC with IVC tumor thrombus,providing much more information for clinical diagnosis and treatment.