Objective To investigate possible nerological origination of rabbit P3a potential. Method we simultaneously recorded the ERPs at dura and various depths beneath the skull in a point of rabbit skull, similarly did some in different points of rabbit skull. Result In the Brodmann 25 and 32 areas(AcgI), the polarity of P3a to those at dura was markedly reverse and the reverse rate of P3a(81.3%) was significantly higher than other locations, the P3a without polarity reversal showed steep voltage gradient. There was an increasing trend of the P3a reversal and moderate voltage gradient nearby the AcgI, but not other regions. Conclusion Rabbit P3a potential possibly originate from the AcgI acea.

Objective To investigate the generative and regulatory effects of AchMR on P 3a and its mechanism in different subregions of rabbit cingulate gyrus. Methods Event related potentials(ERPs) P 3a potentials in Normal, AcgI, AcgⅡand Pcg regions were recorded after microinfusion of 0.5 mmol/L scopolamine at three different time points(instantly, at the 15th minute and at the 30th minute), 2 mmol/L acetylcholine and 1 mmol/L scopolamine. Results The P 3a amplitude decreased and/or P 3a latency increased with scopolamine in time and dose dependent pattern in AcgⅠ, AcgⅡ and Pcg, and P 3a might disappear only in AcgⅠ and the P 3a again be recorded after microinfusion of acetylcholine in AcgⅠ. Conclusion The action of AchMR in AcgⅠ area markedly affects the generation and/or regulation of P 3a and confirms the AcgⅠ is the source of rabbit P 3a potential. The action of neurons in AcgⅠ might facilitate the P 3a potential.

Objective To use event-related potentials to identify whether face recognition is implicit, and to investigate specificity, race and inversion effects in face recognition. Methods Upright and inverted photographs of Eastern and Western human faces, upright dog faces and a mobile phone were presented in random order for 150 ms to 20 healthy subjects. The subject's attention was diverted away to search for the letter ”f ” in 6 small letters below the photos. Behavioral data were recorded synchronous with event-related potentials (ERPs). Results The P100,N170 and P170 potentials were enhanced and accelerated by faces compared with non-face images. The amplitude of P100 had the strongest correlation with the type of stimulus, showing the biggest amplitude with the mobile phone image (9.5 μV ), followed by the dog face (7.5 μV ). The weakest amplitude was evoked by the human face (6.9 μV). The amplitude of N170 responded most strongly to the human face, followed by the mobile phone (0.5 μV) and the dog face ( -1.9 μV). The P170 amplitude was largest with the human face and the latency was shortest (7.8 μV and 162.2 ms), followed by the dog face (5.2 μV, 174.4 ms) and the mobile phone (5.1 μV,173.5 ms). ERP components were not affected by the orientations or races of the faces in this situation. No difference was found in the late positive component evoked by the human faces and other images. Conclusions Implicit face recognition may depend on the specifics of the stimulus, although no race effect or inversion effect was detected using this procedure.

Objective To evaluate the clinic role of diffusion tensor imaging in diagnosis of central nervous system diseases with damage of white matter fibro bands.Methods Ten healthy volunteers and sixty-four patients(31 cases of cerebral vascular disease, 7 cases of demyelinating disease, 7 cases of inflammatory disease, 10 cases of tumor, 2 cases of degenerative disease, 2 cases of congenital brain dysgenesis, 3 cases of brain atrophy, and 2 cases of others) underwent MRI examination, which included conventional T 1WI, T 2WI, T 2 Flair and diffusion tensor. Fractional anisotropy(FA) were measured in white matter tracts and gray matter.Results Ablation of white matter fibro tracts and reduction of FA could be found in 59 patients(92.1%).Erosion like alteration was seen in 12 patients(20.3%).In all the patients with tumors showed push and displacement of the white matter fibro tracts.Conclusion Diffusion tensor imaging can show lesions in white matter at early stage of some diseases.It is better in displaying damage or displacement of white matter tracts than conventional MRI.

This paper reports an in vivo study on the biophysics characteristics of reticulocytes. Anemia was induced by injection of phenylhydrazine in rabbits. The measurements, including electrophoresis rate, hematolytic rate, fluorescent polarization and the changing anisotropic value, were performed in vivo for 72 hours in the process of reticulocytes growing into erythrocytes. It was shown that there were obvious changes in the biophysics characteristics of reticulocytes in this course. Therefore, the findings are of significance to basic, theoretical and clinical studies.
Anemia, Hemolytic ; blood ; chemically induced ; Animals ; Biophysical Phenomena ; Biophysics ; Erythrocyte Deformability ; Erythrocyte Membrane ; physiology ; Phenylhydrazines ; Rabbits ; Reticulocytes ; metabolism ; physiology

The changes in the cellular main components of the mouse erythroleukemia cell line MEL for TFAR19 gene transfection were studied by the technology of Fourier transform infrared spectroscopy (FTIR). Using the method of gene transfection with liposome, we obtained MEL-TF19 cell line, which stably carries TFAR19, a novel apoptosis-related gene. The expression of the gene on mRNA level was confirmed by RT-PCR. Then, FTIR spectra of the cells were measured in the course of apoptosis induced by serum deprivation. Our results indicated that after being transfected with TFAR19 gene, MEL-TF19 cells exhibited relatively higher protein content, higher transcriptional activity, and relatively lower phospholipid content as compared with those exhibited by MEL cells. All the above changes reflect the apoptosis-promoting effect of TFAR19 gene, and maybe account for the cellular rheological changes after TFAR19 gene transfection, which were discovered in our previous study.
Animals ; Apoptosis ; genetics ; Apoptosis Regulatory Proteins ; Cell Line, Tumor ; Genes, Tumor Suppressor ; Leukemia, Erythroblastic, Acute ; genetics ; pathology ; Mice ; Molecular Sequence Data ; Neoplasm Proteins ; genetics ; pharmacology ; Spectroscopy, Fourier Transform Infrared ; Transfection

RBC membrane shear elastic modulus and membrane viscosity are two important indexes reflecting RBC membrane viscoelasticity. Their variation was investigated in this study after rabbits were radiated with l60Co. With a new ektacytometer, we measured the small deformation index (DId) and the half-time of deformation relaxation (t0.5) of RBC in flow field then we calculated RBC membrane shear elastic modulus and membrane viscosity. We found that the value of RBC membrane shear elastic modulus and membrane viscosity continuously increased from 0 to 16th day then continuously decreased and tended to be stable on 60th day or so. The reason may lie in the variation of proportion of new and old RBC in blood and variation of microconformation of RBC membrane after rabbits were radiated with　60Co.