The afferent connections to the nucleus amygdaloideus centralis and nucleusamygdaloideus medialis in 42 albino rats were examined by means of retrogradetransport method of horseradish peroxidase(HRP).The microinjection syringe needle or microelectrophoretic pipette was insertedstereotaxically from vertical or oblipue approaches.The injections of HRP weregiven in the central and medial nuclei of the amygdaloid complex.The results were as follows:Following the injections of HRP into the nucleus amygdaloideus centralis,label-ed cells were always found in prepiriform cortex,periamygdaloid cortex,entorhinalcortex,ventral part the caudoputamen and globus pallidus,nucleus lateralis hy-pothalami,nucleus parafascicularis,corporis geniculati medialis,nucleus raphe dosalis,nucleus parabrachialis,locus coeruleus and zona reticulate substantia nigra.While HRP was injected into the nucleus amygdaloideus medialis,the labeledcells were always found in bed nucleus of stria terminalis,nucleus ventromedialis(hypothalami)and substantia nigra pars compacta.As the injections were given from lateral oblique approaches,labeled cells werenot found in the neo-cortex.There were no labeled cells demonstrated in the nuc-leus solitarius.In the above-mentioned areas nucleus ventromedialis(hypothalami),nucleusparabrachialis and locus coeruleus were often labeled bilaterally while others,ipsila-terally.

Objective:Through the establishment and application of the biosafety autopsy pathology platform in Huoshenshan Hospital, the feasibility and application effect of the biosafety autopsy pathology platform were analyzed.Methods:The feasibility and application effect of the biosafety autopsy pathology platform were analyzed by layout design, instruments and equipment preparation, testing methods examination, and effect evaluation.Results:A total number of 26 cases of systematic autopsy and 8 cases of minimally invasive autopsy (puncture) were performed on the biosafety autopsy pathology platform, and no one was infected. Some pathology original findings were identified, including COVID-19 and pathological characteristics of identification, SARS respiratory failure mechanism and treatment significance, systemic distribution and spreading mechanism of the new coronavirus, the " storm" of inflammation pathological basis, some tumor markers rise in pulmonary pathological cell source and the overcast with fibrosis characteristics, such findings play important roles in the clinical diagnosis and treatment of COVID-19.Conclusions:The study of autopsy pathology is of great significance for the prevention and control of emerging infectious disease, which calls for early intervention. To promote the standard construction of biosafety autopsy platform is the key to the pathological study of emerging infectious diseases. Pathological research and clinical diagnosis and treatment should be combined to inform each other.