1.Establishment and assessment of a visual detection method for nucleic acid of Afri-can swine fever virus
Xingqi LIU ; Yujie BAI ; Mengyao ZHANG ; Jingbo HUANG ; Guangliang LIU ; Yuanyuan LI ; Shuyi TAN ; Haili ZHANG ; Yan ZHANG ; Zongxi CAO ; Hualei WANG ; Pei HUANG
Chinese Journal of Veterinary Science 2024;44(8):1585-1592
African swine fever(ASF),caused by the African swine fever virus(ASFV),is a highly contagious infectious disease of pigs.This disease has been spread rapidly in China since 2018,po-sing a huge threat to China's pig farming industry.To rapid detect the ASFV,a loop-mediated iso-thermal amplification(LAMP)combined with the disposable nucleic acid visualization test strip was established for visual detection of the nucleic acid of ASFV B646L gene.The method was easy to operate without special instruments and equipment,while it effectively avoided the disadvantage of false positives caused by aerosol contamination.The method was able to detect 1.16 copies/μL of the recombinant plasmid in 50 min at 65 ℃.In addition,the method was specific with no cross-re-action with classical swine fever virus,porcine reproductive and respiratory syndrome virus,por-cine parvovirus,transmissible gastroenteritis virus.The results in this study provides a rapid,con-venient,sensitive and reliable method for early diagnosis and screening for ASFV suspected infec-tion cases.
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