Objective: To identify nasal width changes occurring after Le Fort Ⅰosteotomy and to compare prospectively the effect of three surgical techniques for controlling postoperative nasal width . Methods:In the study, 79 patients (22 male and 57 female, mean age 23.2 ±3.4 years), who re-ceived Le Fort Ⅰosteotomy at Peking University Hospital of Stomatology from 2011 to 2014, were ran-domly divided into three groups .Group 1 was treated with traditional intraoral alar base cinch suture ( ABCS);Group 2 with extraoral ABCS, and Group 3 with traditional ABCS plus an extra intraoral suture at points G.lat.All the patients had taken 3D photos using 3dMD camera before operation , and 3, and 6 months after operation.The nasal widths, which were indicated as distances between Sbal-Sbal, G.lat-G.lat and Al-Al, were measured by two examiners in the 3D photos three times with a time-interval of one week .SPSS 13 .0 was used to do the statistic analysis .Results: At the end of the postoperative 6 months, the nasal widths lessened as compared with the postoperative 3 months.No significant diffe-rences were found between the three groups 6 months after the operation .The degree of the postoperative nasal width widening had positive correlation with that of the intraoperative nasal width widening , and had negative correlation with the initial nasal width and the amount of post-suture narrowing .Conclusion:There is no difference between three suturing techniques for controlling nasal width widening after Le FortⅠosteotomy.The postoperative nasal width-widening can’t be totally avoided, and the alteration might last at least 6 months after the operation .For patients with narrow nasal width and need to move maxilla forward , more overcorrection of ABCS is needed to control the postoperative nasal base widening .

OBJECTIVETo isolate and identify the anamorph of Shiraia bambusicola.

METHODFungus strains were isolated from mature ascospores and stroma. They were identified by means of morphological identification including colony and microscope characteristic, the molecular identification was done by 5.8S-ITS rDNA.

RESULTThe strains GZDXIFR-171 and GZDXIFR-181 were isolated and obtained with the separation of different methods, which had the same colony morphology. With the universal primers of the ITS1-5.8S rDNA-ITS2, the 5.8S-ITS rDNA sequence of GZDXIFR-171 and GZDXIFR-181 were obtained by the PCR amplification and sequencing. Compared with the published nucleotide sequence of 5.8S-ITS rDNA in NCBI (National Center for Biotechnology Information), GZDXIFR-171 and GZDXIFR-181 were highly identical with S. bambusicola.

CONCLUSIONThe isolated strains GZDXIFR-171 and GZDXIFR-181 were confirmed to be the true anamorph of S. bambusicola.

Ascomycota ; classification ; genetics ; isolation & purification ; DNA, Fungal ; genetics ; DNA, Ribosomal Spacer ; genetics ; Molecular Sequence Data ; Mycological Typing Techniques ; Phylogeny ; RNA, Ribosomal, 5.8S ; genetics

OBJECTIVEBy examining the colony growth rate, colony texture, substrate mycelium color and separation method of 40 anamorph strains of Shiraia bambusicola to investigate the relationship between these indexes and content of hypocrellin A.

METHODIn UV-spectrophotometry and microscopic observation were applied in the experiments. The results were analyzed by the clustering and variance analysis.

RESULTSPSS clustering analysis showed that the colony characters of S. bambusicola anamorph strains had significant differences with hypocrellin A content.

CONCLUSIONIn the tested conditions, substrate mycelium color of S. bambusicola anamophs has the most distinct correlation with the yield of hypocrellin A.

Ascomycota ; growth & development ; isolation & purification ; metabolism ; Cluster Analysis ; Mycelium ; growth & development ; isolation & purification ; metabolism ; Perylene ; analogs & derivatives ; metabolism ; Pigmentation ; Quinones ; metabolism ; Spectrophotometry, Ultraviolet

UNLABELLEDTo culture silkworm Cordyceps cicadidae artificially.

METHODTwenty Paecilomyces cicadae were collected and isolated from 8 natural reserves in China and the Cheju Island of Korea. By the impregnation method, their infection to silkworm larvaes and silkworm chrysalises and the synneneta production were studied.

RESULTThe results showed that all P. cicadiae strains could infect silkworm larvaes and silkworm chrysalises, and some strains could produce synnenetas.

CONCLUSIONThe silkworm chrysalis was better than silkworm larvae to culture C. cicadidae.

Animals ; Bombyx ; growth & development ; microbiology ; Cordyceps ; growth & development ; Culture Techniques ; methods ; Larva ; microbiology

Portal hypertension is a common physiopathological change in liver cirrhosis. In this study, rabbits were used and the model of pre-hepatic portal hypertension (PHT) was induced by partial ligation of portal vein in two steps. We measured the diameters of portal vein and small mesenteric vein at different time-points. Then we detected the stress forces induced by blood flow in varicose veins and in portal vein; such forces included hydrostatic pressure, shear stress and circumferential stress. With the increase of the diameter of varicose small mesenteric vein, the hydrostatic pressure and circumferential stress gradually elevated and shear stress descended markedly in both the portal vein and the small mesenteric vein of PHT rabbits, between which there was a positive linear correlation. The findings in our study indicate that the complications of PHT are partially attributable to the environment of lower shear stress and higher circumferential stress in which the blood vessels of portal venous system live.
Animals ; Hemodynamics ; Hypertension, Portal ; etiology ; pathology ; physiopathology ; Mesenteric Veins ; pathology ; physiopathology ; Portal Vein ; pathology ; physiopathology ; Rabbits ; Stress, Mechanical ; Vascular Resistance ; physiology ; Venous Pressure

In recent years, gene engineering is developing rapidly and many recombinant proteins have been expressed. The use of plant bioreactor to express specific pharmaceutical proteins provides a new way for the prevention and treatment of some important diseases in human beings. Nowadays, chloroplast genetic transformation and expression system has become a research hotspot in plant bioreactor. Higher plant chloroplasts have unique advantages in the expression of recombinant proteins due to their special structures and inherited characteristics: such as high expression, site-specific integration, and the maternal inheritance characteristics of exogenous genes. The maternal inheritance of chloroplast is helpful for biological safety of transgene escaping by pollens. Many important pharmaceutical proteins have been successfully expressed in plant chloroplasts. As a chloroplast transformation model of higher plants, tobacco has made significant progress in the expression of pharmaceutical proteins, such as vaccine antigens, antibodies, and other important recombinant proteins. Chloroplast genetic transformation in higher plants also provides new techniques and methods for the study of chloroplast gene expression and regulation mechanisms. In order to provide a new idea for the development of chloroplast expression platform and the expression of important pharmaceutical proteins, this review outlined the progress of chloroplast genetic transformation system in higher plants, including the chloroplast transformation principle, vector construction, expression of recombinant proteins and important pharmaceutical proteins, and the effects of recombinant proteins expression on plant metabolism and traits.

Ganoderma lucidum is a valuable medical macrofungus with a myriad of diverse secondary metabolites, in which triterpenoids are the major constituents. This paper introduced the germplasm resources of genus Ganoderma from textual research, its distribution and identification at the molecular level. Also we overviewed G. lucidum in the components, the biological activities and biosynthetic pathways of ganoderic acid, aiming to provide scientific evidence for the development and utilization of G. lucidum germplasm resources and the biosynthesis of ganoderic acid.