The cortexes were obtained from new-born rats and dissociated to single cells by triturating. The cells were cultured in neural stem cell (NSC) culture medium (DMEM supplemented with bFGF, EGF and B27) and formed primary neurospheres after 7 days. Single cells dissociated from neurosphere were cultured in 96-well plates and formed single-cell cloning neurosphere 7 days later. The primary and single-cell cloning neurospheres were both positive for the immunofluorescent staining of nestin and were identified as NSC. It was proved that NSC can be expanded in vitro and provide seed cells for neural tissue engineering.
Animals, Newborn ; Cell Separation ; Cells, Cultured ; Cerebral Cortex/*cytology ; Culture Media ; Neurons/*cytology ; Rats, Sprague-Dawley ; Stem Cells/*cytology ; Tissue Engineering

Objective To study the closure time of neonatal ductus venosus and the Z score regression equation, and to explore the variation of closure time of neonatal ductus venosus with gestationalage.Methods Color doppler ultrasound was applied to detect the closure time of neonatal ductus venosus in normal newborns, Z score regression equation ( lnY =a +bX +cX2 ) for the closure time of neonatal ductus venosus ( Y ) was developed by regression analysis which used gestational age ( X) as an independent variable. The Z scores of the closure time of neonatal ductus venosus in different gestational age were calculated by the formula [ Z = ( M - Y )/S x , M for observation value, Y for predictivevalue].Results There were 432 cases in our study.The closure time of neonatal ductus venosus was negatively related to gestational age ( r = -0. 938 , P <0. 001 ) . The nonlinear regression equation was lnY= -5. 228+0. 089X-0. 000228X2, R2 =0. 854, Sx =0. 214(P<0. 001). Based on the predicted mean of the closure time and Sx related to different gestational age, Z scores for specific closure time of neonatal ductus venosus could be calculated by the formula [ Z =( M -Y)/Sx ] . The Z scores were normally distributed,and did not change with gestational age.Conclusions Theclosure & nbsp;time of neonatal ductus venosus is negatively related to gestational age. The Z scores obtained by the predicted nonlinear regression equation are normally distributed.

Objective: To explore the correalation between change of postoperative cognitive function and injury of the draining veins around meningiomas in superficial middle cerebral vein areas, to discuss the importance and protection method of draining veins around meningiomas in order to guide the microneurosurgery. Methods: From July 2013 to July 2017, the clinical data of 54 patients with superficial middle cerebral vein areas meningiomas(tumor group) in the Central Hospital of Guangdong Nongken were retrospectively analyzed.And 52 healthy volunteers were selected as the control group.The preoperative and postoperative cognitive function and meningiomas peritumoral edema(MPE) were assessed by the Montreal Cognitive Assessment(MoCA) and CT/MRI. Results: The scores of visuospatial and executive[(3.23±1.27)points], order[(2.52±1.27)points], memory[(2.20±1.14)points], attention[(4.71±0.97)points], language[(2.19±0.74)points], abstract[(1.43±0.63)points], location[(5.83±0.42)points], total[(22.06±0.33)points] in the tumor group were significantly lower than those in the control group[(4.83±0.38)points, (3.0±0.02)points, (3.5±1.04)points, (5.98±0.14)points, (2.54±0.50)points, (1.88±0.38)points, (5.98±0.33)points, (27.83±0.16)points](t=4.504, 6.116, 9.338, 2.782, 4.509, 2.390, 8.670, all P<0.05). According to the injury of the draining veins around meningioma, 54 patients were divided into the two groups, 43 cases in the no-injuried group, 11 cases in the injuried group.There were no statistically significant differences between the no-injuried group and injuried group in all measurements before operation(all P>0.05). Both no-injuried group and injuried group had decline in MoCA and increase in MPE at 5 days after surgery.Both no-injuried group and injuried group had rise in MoCA[(26.35±0.36)points vs.(22.00±0.67)points] and decrease in MPE[(23.95±4.34)cm³ vs (44.64±9.68)cm³] at 30 days after surgery(t=5.944, 2.098, all P<0.05). The MoCA[(22.59±0.31)points vs (26.35±0.36)points] was obviously increased and the MPE[(87.84±12.78)cm³ vs.(23.95±4.34)cm³] was obviously increased in 30 days after operation in no-injuried group(t=7.289, 5.014, all P<0.05), but the injuried group had just the opposite. Conclusion Injury of the draining veins around meningiomas in superficial middle cerebral vein areas can lead to cognitive dysfunction and compromise the quality of postoperative life in patients, every effort should be undertaken to preserve the draining veins around meningioma.

The cortexes were obtained from new-born rats and dissociated to single cells by triturating. The cells were cultured in neural stem cell (NSC) culture medium (DMEM supplemented with bFGF, EGF and B27) and formed primary neurospheres after 7 days. Single cells dissociated from neurosphere were cultured in 96-well plates and formed single-cell cloning neurosphere 7 days later. The primary and single-cell cloning neurospheres were both positive for the immunofluorescent staining of nestin and were identified as NSC. It was proved that NSC can be expanded in vitro and provide seed cells for neural tissue engineering.
Animals ; Animals, Newborn ; Cell Separation ; Cells, Cultured ; Cerebral Cortex ; cytology ; Culture Media ; Neurons ; cytology ; Rats ; Rats, Sprague-Dawley ; Stem Cells ; cytology ; Tissue Engineering

Objective To analyze whether preoperative serum thyroglobulin (Tg) can be the indicator for predicting malignancy in follicular thyroid tumors (FTC).Methods A retrospective analysis was done for 125 patients with FTC who had been diagnosed by pathology in our hospital.The patients were grouped into benign group and malignant group.Sex,age,tumor size and preoperative serum Tg concentration were analyzed.Results There were 62 patients in benign group (23 male and 39 female).The mean age was 49 years old,ranging from 19 to 78 years,and the mean diameter of tumors was (3.4±1.374) cm,ranging from 0.1 to 5.5 cm.There were 63 patients in malignant group (13 male and 50 female).The mean age was 46 years old,ranging from 15 to 79 years,and the mean diameter of tumors was (3.14±1.143) cm,ranging from 0.3 to 7 cm.The mean preoperative serum Tg concentration was (299.73± 495.02) ng/ml in malignant group and(48.20 ±43.68)ng/ml in the benign group.Through comparing the two groups,we found age and tumors diameter had no statistical difference between the two groups (P＞0.05),while sex and Tg had statistical difference (P＜0.05).When the cutoff of Tg was 100 ng/ml,the sensitivity and specificity for the diagnosis of malignancy was 48.7％ and 90％ respectively.Conclusions Tg can be used as auxiliary diagnosis index of thyroid FTC.High preoperative Tg levels should be highly suspected the possibility of FTC.

Objective Glioma stem cells (GSCs) are the root causes ofglioblastoma recurrence.Glioma expresses low level of micro RNA 124 (miR-124), which plays an important role in the development of glioma.In this study, we explore the mechanisms miR-124 by overexpressing miR-124 in GSCs.Methods Human GSCs were transfected with lentivirus mediated miR-124 overexpression vector (pGC-miR-124-GSCs group), and GSCs in normal culture were used as control group.Cell proliferation was accessed by MTT assay, and stem cell phenotype was analyzed by flow cytometry.Quantitative real time PCR was employed to detect the miR-124 expression and its target genes (A kt and RelA) expressions;and ELISA was carried out to detect the secretions of downstream inflammatory cytokines interleukin (IL)-1 and IL-8.Results The lentiviral miR-124 expression vector was constructed successfully and transfected into human GSCs (pGC-miR-124-GSCs).The proliferative capacity of cells in the pGC-miR-124-GSCs group was significantly lower than that in the control cells (P＜0.05).CD133 positive rate was statistically decreased, the miR-124 expression was significantly increased, the A kt and RelA expressions were significantly decreased, and correspondingly the secretions of IL-1and IL-8 were significantly reduced in the pGC-miR-124-GSCs group as compared with those in the control group (P＜0.05).Conclusion MiR-124 overexpression induces GSCs differentiation and activates a strong anti-tumor ability, whose mechanism may be related to inhibition of inflammatory cytokine production.

Objective:To investigate the curative efficacy of X-ray stereotactic radiotherapy combined with temozolomide in the treatment of recurrent glioma.Methods:48 patients with recurrent glioma treated in Mianyang Central Hospital from January 2018 to January 2019 were retrospectively selected, including 24 patients treated with stereotactic radiotherapy as the control group and 24 patients treated with temozolomide combined with stereotactic radiotherapy as the observation group. The treatment effect, inflammatory factor level, incidence of adverse events and survival rate were compared between the two groups.Results:The complete remission rate and total effective rate in the observation group were significantly higher than those in the control group (66.7% vs 37.5%, 87.5% vs 62.5%) (all P<0.05). There were no significant differences in serum levels of hepatocyte growth factor (HGF), tumor necrosis factor-α (TNF-α) and interleukin-17 (IL-17) between the two groups before treatment (all P>0.05). After treatment, the serum levels of HGF, TNF-α and IL-17 in observation group was significantly lower than those in control group (all P<0.05). The incidence of adverse events in the observation group was significantly lower than that in the control group ( P<0.05). During follow-up of 6, 12 and 18 months, the survival rate of patients in the observation group was significantly higher than that in the control group, with statistically significant difference (all P<0.05). Conclusions:The combination of X-ray stereotactic radiotherapy with temozolomide in the treatment of recurrent glioma shows better clinical outcome and extended survival rate. To conclude, this combined treatment is recommended in further clinical promotion.