Objective To determine sensitive indices for defining degenerative cervical spinal canal stenosis(DCSCS) with a new radiograghic measurement method. Methods One hundred normal lateral radiographs of the cervical spine were divided into two groups according to ages. The following indices were measured or calculated: sagittal diameter of spinal canal(a), sagittal diameter of cervical body(b), sagittal diameter of edge or degenerative cervical body(c), cervical spinal canal ratio[(a/b),CSCR] and effective cervical spinal canal ratio [(a+b-c)/c,ECSCR]. The comparisons of these indices between two groups were performed to select the most sensitive index for clinical reference. Results There was no significant difference between the two age groups in terms of a, b and a/b. The comparison of c and (a+b-c)/c between two age groups showed significant difference at the level of C4,C5 and C6 (P

AIM: To observe the effects of Huzhang Decoction(HZD)(Rhizoma Polyqonicuspidati,Radix Sanguisorbae,Herba Saururi,Rhizoma curcumae,etc) on tumor necrosis factor ?(TNF-?)and apoptosis in liver injury induced by CCl_4 in rats. METHODS: Wistar rats were treated with HZD(1.0g/kg or 2.0g/kg) after 3days by administration of CCl_4(1 ml/kg,po).The levels of TNF-? in serum were measured by enzyme-linked immunosorbent assay(ELISA).The apoptosis cells in liver tissue were tested by TdT-mediated digoxin-dUTP nick end labeling(TUNEL),and the positive expression of bcl-2 or bax by strept avidin-biotin complex(SABC). RESULTS: After being treated by HZD the serum level of TNF-? and apoptosis index was decreased obviously in rat with liver injury induced by CCl_4,and the ratio of bcl-2/bax was increased markedly(compared with liver injury group,P

Objective To study the effect of Sildenafil in the treatment of hypoxemia after operation of cyanotic congenital heart disease.Methods A total of 34 cases with hypoxemia after operation of cyanotic congenital heart disease were collected between January 2009 to January 2015.They took Sildenafil with the dosage of 0.3-0.5 mg/kg every 6 hour.The change of heart rate (HR),systolic blood pressure (SBP),central venous pressure (CVP),pulmonary artery pressure (PAP),arterial partial pressure of oxygen [pa (O2)],arterial partial pressure of carbon dioxide [Pa (CO2)],oxygenation index [pa (O2)/FiO2],peak airway pressure (Ppeak),positive inotropic drug score (PIDS) and the lower bound of liver were observed at 4 time points,including before taking the medicine,after taking the medicine for 1 hour,after taking the medicine 1 day and after the ventilator was removed.Based on the data,the therapeutic effects of Sildenafil were studied.Results All 34 cases were cured.At the time point of 1 hour after taking Sildenafil,PAP,CVP and Ppeak decreased significantly,but pa (O2) and pa (O2)/FiO2 increased significantly (P ＜ 0.05).At the time point of 1 day after taking Sildinafil,SBP increased steadily,but PAP and CVP continued to get lower,and PIDS decreased significantly,while the liver lower bound was significantly reduced (P ＜ 0.05).At the time point of removing the ventilator,pa (O2) reached to (144.12 ± 26.25) mmHg,and hypoxemia was corrected,but PAP was reduced to (37.47 ± 3.77) mmHg,PIDS decreased to (17.56-± 1.94) scores,and heart failure was corrected.Compared with the previous 3 time points,there were statistically significant differences (P ＜ 0.05).Conclusion Sildenafil can highly selectively decrease pulmonary vascular resistance and obviously promote the correction of hypoxemia after operation of cyanotic congenital heart disease.

Objective:To evaluate the value of gastrointestinal endoscopy in patients with hepatic cell cancer.Methods:A retrospective analysis was made on 316 patients with hepatic cell cancer undergoing gastrointestinal endoscopy preoperatively from 1992 to 1998.Results:2 cases were accompanied with gastric cancer,62 cases with esophagogastric varices,58 cases with gastric or duodenal ulcer,218 cases with gastritis,Of them 30 cases with portal hypertension gastropathy,24 cases were normal.Conclusion:Hepatic cell cancer is usually accompanied with upper gastrointestinal lesions,It should be paied attention to treat perioperatively.

Objective To evaluate hepatic enhancement technology with MRI and its clinical application.Methods 147 patients underwent enhanced MRI scanning and an automatic injector.Total dose of contrast agent for each patient was 10 to 15ml(0.2mmol/kg)at a rate of 2ml/s,Two-phase enhancement were performed at a delay of 30 and 70 seconds respectively after the initiation of bolus injection.Results Good visualization of two phases for both hepatic artery and portal vein were achieved in 147 patients in addition to branches of abdominal aorta,hepatic artery,splenic artery,portal vein,inferior vena cava and vasculatures in hepatic port.The 4 patients did not have ideal two-phase images for various reasons.Conclusion MRI with bolus injection using an automatic injector can satisfy the diagnosis for hepatic diseases.

Objective To evaluate the significance of hemihepatic vascular occlusion with extrahepatic control of major hepatic veins for hepatectomy in cirrhotic carcinoma. Methods A retrospective comparative study for hepatectomy in patients with hepatocellular carcinoma(HCC) using Pringle maneuver (groupA,n=44),hemihepatic vascular occlusion(group B,n=76) and hemihepatic vascular occlusion plus extrahepatic control of major hepatic veins(group C,n=85)were made from March 2006 to September 2008.The amount of intraoperative bleeding,time of operation,postoperative liver function,liver function recovering and complications were compared.Results There was significant difference in the amount of intraoperative blood loss between the three groups(543.7 ml、415.8 ml、324.5 ml,respectively,F=98.96,P<0.001).There was no difference in the time of operation.The level of serum alanine transaminase(ALT) and that of serum bilirubin on the 3rd and 6th day postoperatively in group B,and C was significantly lower than that in group A.Conclusions Hemihepatic vascular occlusion with control of major hepatic veins results in selective liver isolation from the systemic circulation,which is more effective than Pringle maneuver for controlling intraoperative bleeding without interruption of hemodynamic stability in liver cancer patients.

Objective To study on NGF positive cells changes by the action of Danshen and explore the correlation between time and amount of NGF after traumatic brain injury (TBI)in rat.Methods72 rats were randomly divided into 3 groups,including sham group,brain injury and Danshen therapy group.TBI rat models were made by fluid percussion.NGF was determined on the 3rd,7th and 14th day after TBI.ResultsNGF positive cells after traumatic brain injury in brain reached the maximum level at the 3rd day(1.29±0.61),and it decreased since the 7th day(2.03±0.42),and it decreased to the lowest level on the 14th day (0.87±0.23).NGF positive cells after traumatic brain injury treated by Danshen were more than those patients who were treated without Danshen(1.77±0.54,3.35±0.37,0.65±0.21 vs 0.75±0.31,1.58±0.52,0.49±0.36,P＜0.05～0.01).ConclusionsThe expression of NGF was significantly increased in brain damaged tissues.This may be served as a useful marker of diagnosis in time-course of brain leison.Danshen injection can be used to protect brain after traumatic brain injury.

Background With the progression of cortical cataract,the lens thickness and anterior chamber will change and may have significant influences on the structure and function of the eyes.A special measurement is needed to observe the anterior and posterior surface of lens and detailed angle structure,and to quantify these changes.Ohjective This study was to compare the lens thickness and angle structures by CASIA SS-1000 anterior segment optical coherence tomography (AS-OCT) in early and intumescent stage of cataract and quantify the ocular anatomical differences.Methods An observational study was performed.Fifty cortical cataract patients (87 eyes) of the early stage or the intumescent stage in the ophthalmology outpatient clinic of Daping Hospital from October 16 to October 30,2015 were continuously enrolled in this research.The eyes were assigned to the early stage group (28 patients 44 eyes) and the intumescent stage group (22 patients 43 eyes) according to the slit lamp examination results.The average age was (56.46 ± 12.90) years in the early stage group and (70.95 ± 8.81) years in the intumescent stage group.With the use of CASIA SS-1000 AS-OCT measurements,lens thickness,angle opening distance (AOD),angle recess area (ARA),trabecular-iris space area (TISA),trabecular-iris angle (TIA) and anterior chamber depth (ACD) were measured and compared between the two groups.Results Lens thickness was (4.438±0.487) mm in the early stage group and (5.094±0.451)mm in the intumescent stage group,with an average increase of (0.656±0.101) mm,showing a significant difference between the two groups (t =-6.513,P =0.000).ACD in the early stage group was (2.966±0.326)mm and (2.534±0.376)mm in the intumescent stage group,with an average decrease of (0.432±0.075)mm,showing a significant difference between the two groups (t =5.730,P =0.000).AOD500,AOD750,ARA500,ARA750,TISA500,TISA750,TIA500 and TIA750 were all lower in the intumescent stage group than those in the early stage group,with significant differences between the two groups (all at P=0.000).Conclusions The lens was thicker,ACD was shallower and anterior angle was narrower in the intumescent stage than those in the early stage of cortical cataract patients.SS-1000 AS-OCT is efficiency with high definition,and is supposed to have promising prospect of clinical application.

Vernalization makes Arabidopsis and other cruciferous plants flowering earlier. During this process, an important plant homeodomain-finger(PHD-finger) protein named VIN3 is involved. The PHD domain was a conserved zinc-finger domain in eukaryotic organism. It used to take part in the interaction between proteins, especially the modification on histone of nucleosome, such as methylation, acetylation and phosphorylation. In vernaliazation pathway, the proteins translated by VERNALIZATION INSENSITIVE 3(VIN3) and homologous genes could result in methylation on H3K9 and H3K27 and deacetylation on H3K9 and H3K14 on chromatin histone of FLOWERING LOCUS C, a gene that inhibited flowering. The structure state of FLC would be changed from relaxation into compression. Then the transcription activity of FLC could be restrained and it couldn't inhibit flowering any more, so it would induce flowering earlier. This paper reviewed the function of PHD-finger proteins in vernalization pathway in Arabidopsis and other cruciferous plants, and overviewed the vernalization mechanism.
Amino Acid Sequence ; Arabidopsis ; genetics ; metabolism ; Arabidopsis Proteins ; genetics ; metabolism ; physiology ; Brassicaceae ; genetics ; DNA-Binding Proteins ; genetics ; metabolism ; physiology ; Gene Expression Regulation, Plant ; genetics ; physiology ; Histones ; metabolism ; Homeodomain Proteins ; genetics ; metabolism ; physiology ; MADS Domain Proteins ; genetics ; metabolism ; Molecular Sequence Data ; Transcription Factors ; genetics ; metabolism ; physiology ; Zinc Fingers

Lipoxygenases (linoleate: oxygen oxidoreductase, EC 1.13.11.12; LOXs) are encoded by a multi-gene family in plants. The LOXs are monomeric non-heme, non-sulfur iron dioxygenases, which catalyze the incorporation of molecular oxygen into polyunsaturated fatty acids containing a cis, cis-1,4-pentadiene moiety. The LOX isoforms are distinguished by differences in optimum pH of the reaction, pI, substrate and product specificity, spatial and temporal expression, and subcellular localization. The function of various LOXs in plants has been suggested. Some of the physiological processes in which lipoxygenases have been implicated include wounding, pathogen attack, seed germination, fruit ripening, plant senescence, and synthesis of Abscisic acid (ABA) and Jasmonic acid (JA). During normal vegetative and reproductive growth, lipoxygenases have also been suggested to act as vegetative storage proteins, participate in transference of lipoid, and response to nutrient stress and source/sink relationships. Significant progress in understanding LOX families will be beneficial to the application of the LOX in crop breeding, research on new-type phytoalexin and food industry.
Gene Expression Regulation, Plant ; Lipoxygenase ; genetics ; metabolism ; Multigene Family ; Plants ; enzymology ; Protein Isoforms ; genetics ; metabolism