Adult ; Female ; Humans ; Male ; Medical Staff, Hospital ; Middle Aged ; Occupational Diseases ; epidemiology ; Urticaria ; epidemiology ; Young Adult

AIM: To prepare the efficient tumor-DC vaccines, dendritic cells(DC) derived from 6-8 weeks Balb/c mice bone marrow progenitor cells were pulsed by apoptotic SP2/0 tumor cells and induced maturation by SP2/0 tumor lysates supernatants. Then SP2/0 tumor burdening Balb/c mice were immunized by the tumor-DC vaccines to observe the therapeutic effects in vivo .METHODS: Immature DC were derived by recombinant murine GM-CSF and IL-4, then were pulsed by SP2/0 apoptotic cells. Tumor-DC vaccines were stimulated by LPS and SP2/0 tumor lysates supernatants prepared by four cycles repetitive freezing and thawing, respectively. -thymidine incorporation test and standard 4h [ 51 Cr] release assay were used to detect the proliferation and activation of cytotoxic T lymphocytes (CTL) stimulated by DC in vitro . (4-5)?10 5 DC were immunized in the right inguen of SP2/0 tumor burdening Balb/c mice and most mice received three cycles immunization every two weeks. Changes of the tumor and mice life-spans were recorded. RESULTS: In vitro proliferation and activation of CTL induced by the tumor-DC vaccines of tumor lysates supernatants or LPS stimulation group were more powerful than other groups ( P

Objective:To explore the inhibitory effect of Tangshenping (TSP) on pyroptosis in a streptozotocin-induced diabetic nephropathy (DN) rat model.Methods:DN was established in Sprague-Dawley rats.Rats were randomly divided into DN (model group),irbesartan,and TSP low-,medium-,and high-dose groups,besides the control group.The 24 h albuminuria content,and serum content of TC,TGs,Scr,IL-1β,UREA,LDLs,and IL-18 were assessed.Hematoxylin & eosin and Mallory staining were performed to examine pathological changes in the kidney.The mRNA and protein expression of NLRP3,caspase 1,and GSDMD in the kidney were also examined.Results:The 24 h albuminuria content was obviously lower in the treatment groups compared to the model group (all P ＜.01).Levels of TC,TGs,Scr,UREA,LDLs,and IL-18 after drug interventions were obviously lower compared to the model group (all P ＜.05).The serum content of IL-1β in the TSP me-dium-and high-dose groups were much lower compared to the model group (P =.013 and P =.001,respectively).Through immunohistochemistry and western blotting,we observed that the protein ex-pressions of NLRP3,caspase-1,GSDMD,IL-1 β,and IL-18 were lower after drug interventions compared to the model group (all P ＜.05).Using qPCR,we observed that the mRNA expressions of caspase-1,IL-1β,IL-18,and GSDMD after drug interventions were significantly lower compared to the model group (all P ＜.05).The mRNA expressions of NLRP3 in the TSP medium-and high-dose groups were both lower compared to the model group (all P ＜.05).Conclusion:TSP downregulated mRNA and protein expressions of NLRP3,caspase-1,and GSDMD.Our findings demonstrate that the beneficial effects of TSP on renal function are at least partly mediated by the inhibition of micro-inflammation and modulation of the expression of pyroptosis-related factors.