Objective To investigate the early diagnosis and disease evaluation value in patients with acute pancreatitis by various serum cytokines measurement.Methods Forty-eight acute pancreatitis patients were divided into two groups based on the results of computed tomography (CT) examination:mild acute pancreatitis group (30 cases) and severe acute pancreatitis group (18 cases).The other 30 normal persons were selected as control group.The various serum cytokines were measured by enzyme-linked immunosorbent assay (ELISA).Results The serum concentrations of interleukin(IL)-1,IL-10 and tumor necrosis factor (TNF)-α in mild acute pancreatitis group were significantly higher than those in control group [(25.00 ± 1.92) ng/L vs.(10.08 ± 2.65) ng/L,(59.78 ± 4.51) ng/L vs.(1.80 ± 0.66) ng/L,(55.31 ± 8.54) ng/L vs.(18.72 ± 7.84) ng/L,P ＜ 0.05].The serum concentrations of IL-1,IL-6,IL-8,TNF-α and platelet activating factor (PAF) in severe acute pancreatitis group were significantly higher than those in mild acute pancreatitis group [(93.27 ± 3.98) ng/L vs.(25.00 ± 1.92) ng/L,(397.84 ± 13.05) ng/L vs.(34.12 ± 4.96) ng/L,(93.32 ±3.40) ng/Lvs.(13.06± 1.86) ng/L,(181.94 ±7.54) ng/Lvs.(55.31 ±8.54) ng/L,(284.53 ±7.88) ng/L vs.(175.25 ±30.15) ng/L,P＜0.05].Conclusion The various serum cytokines measurement has great importance on the early diagnosis of acute pancreatitis and discrimination between the mild acute pancreatitis and severe acute pancreatitis.

Objective To investigate whether the effect of rabeprazole in treating gastroesophageal reflux disease is related to CYP2C19 gene polymorphisms.Methods 278 patients with gastroesophageal reflux disease confirmed by endoscopy and proton-pump inhibitor testing were enrolled in this study,including non erosive reflux disease (NERD) in 122 cases,the reflux esophagitis (RE) in 98 cases and Barrett esophagus in 58 cases.They were treated with rabeprazole for 8 weeks.GerdQ scores before and after the treatment were completed,endoscopy was performed again in patients with RE after treatment.The blood CYP2C19 genotyping was detected by matrix assisted laser desorption ionization time of flight mass spectrum(MALDI-TOF-MS).Results According to the genotype of CYP2C19,they were divided into extensive metabolizers,intermediate metabolizers and poor metabolizers,accounted for 39.57%,42.45% and 17.98%,respectively.There was no significant difference in GerdQ scores of three groups before treatment,and also had no significant difference after 8 weeks treatment,but in each subgroup GerdQ scores after treatment was decreased significantly than before treatment.The total effective rate of 98 patients with RE by endoscopy was 86.73%,but there was no significant difference in total effective rate of the three groups after treatment.Conclusion Rabeprazole is effective in the treatment of gastroesophageal reflux disease.Moreover,rabeprazole is less affected by CYP2C19 genotype and therefore its curative effect is more stable.

Objective To study the relationship among the genome mutation,viral protein structures and virus evolution of human Bocavirus 2(HBoV2).Methods A method of PCR was applied to detect HBoV2 genome in 205 fecal specimens collected from children who had diarrhea in Ruian region,Zhejiang province,China.HBoV2-positive specimens were chosen to further amplify the HBoV2 genome.All the tertiary structures were predicted using the Swiss-PdbViewer software,and a phylogenetic analysis was performed by the MEGA software (version 5.1).Result The positive rate of HBoV2 PCR detection in the collected 205 fecal specimens was 5.36％ (11/205).We obtained a sequence of a nearly full-length HBoV2 genome named as Ruian isolate,which is 5196 bases in length.Alignment of the Ruian HBoV2 sequence with other HBoV2 sequences in GenBank revealed that the new HBoV2 shared 99.79％ similarities with the reference genome (GenBank no NC_012042).No mutation was found in the NP1 gene,whereas sense mutation of NS1,VP1 and VP2 gene were found.Residue solvent accessibilities that relates to hydrophobic interactions were changed between the NS1 of the Ruian isolate and the reference isolate.The protein structure and location of H-bonds were different between the NS1 of the Ruian isolate and the reference isolate.The phylogenetic tree shows a clear division of the NS1,which has a potential evolution toward or a closely evolutionary relationship with the NS1 of HBoV4.Conclusion HBoV2 infection was detected in Ruian region,Zhejiang province.We found sense mutations in the HBoV2 genome of Ruian isolate.These mutations may alter HBoV2 protein structures,and may have an evolutionary significance.These findings provide data of HBoV2 genome mutation.