1.COMPARISON IN SUSCEPTIBILITY OF ANOPHELES DIRVS AND ANOPHELES STEPHENSI TO B STRAIN OF PLASMODIUM CYNOMOLGI
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(03):-
This paper reports on the comparative susceptibility of A. dirus (Hainan strain) and A. stephensi (Hor strain) to the B strain of P. cynomolgi in paired feeding experi-ments. In the most susceptible infective period, the infection rate in midgut and salivary gland of the two species was over 90 %, the difference is not statistically significant.In relatively infective period, three experiments were performed, the infection rate in midgut of A. dirus was 24.4-60.4% with an average of 46.5% (53/114), and that of A. stephensi was 11.5-21.1% with an average of 14.2% (16/113). Statistically, experiment I showed no significant difference, the difference in experiment n was highly significant (P
2.A Study on Laboratory Rearing of Anopheles dirus
Fuzhen CHEN ; Lianzhu LIU ; Zongchen SONG ; Chongying FENG ; Fusheng HUANG
Journal of Third Military Medical University 1984;0(01):-
Anopheles dims Peyton et Harrison, 1979 is an important malaria vector in Southeast Asia and China particularly in forested hilly zones. To establisha a laboratory mating colony for this mosquito is one of the subjects to be solved urgently in malaria research.This paper is to report our experience on breeding this mosquito. Ecological environment is created to simulate the natural conditions. The room-temperature of the insectary is 25?-28℃ and its relative humidity is around 80%.Mosquitoes are kept in cages with the si2e of 50 ? 50 ? 100cm. The larvae are fed with a mixture of de-fatted pig liver powder and yeast with a ratio of 1:3. The adults are fed with a mixture syrup containing 4% of orange juice, 10% of sucrose and other materials. The insectary is illuminated with a 3o w fluorescent light in the day time and a 15 w blue light in the night continuously to induce mating.The mating rate of the fourteenth generation reached 68% and the descendants bred can be used in research work now.