1.Peripheral blood determination of AFP mRNA in patients with recurrent hepatocellular carcinoma (HCC) undergoing percutaneous ethanol injection (PEI)
Yang LIU ; Ming ZONG ; Baihe ZHANG
Chinese Journal of General Surgery 1997;0(04):-
Objective To study the effect of PEI on disseminating HCC cells in the peripheral blood in patients with recurrent HCC. MethodsWe examined blood samples from 29 recurrent HCC patients by nested RT-PCR for the determination of AFP?mRNA before and 1, 2, 3 and 4 weeks after PEI.ResultsBefore PEI 14 (48%) patients out of the 29 cases with recurrent HCC were positive with AFP?mRNA. Four weeks after a course of PEI only 2 cases (7%) remained AFP?mRNA positive.ConclutionsPEI effectively eradicating disseminating HCC cells in the peripheral blood shows a favourable potential for the treatment of recurrent HCC.
2.Case of primary facial spasm.
Qiong LIU ; Zong-Bao YANG ; Chen-Guang WANG
Chinese Acupuncture & Moxibustion 2014;34(12):1160-1160
3.Case of primary hyperhidrosis.
Qiong LIU ; Zong-bao YANG ; Chen-guang WANG
Chinese Acupuncture & Moxibustion 2014;34(10):1040-1040
4.Effect of thymic factor on oxygen free radical and antioxidans in old male rats.
Xiao-Peng LIU ; Zong-Gen PENG ; Gui-Yang GUO
Chinese Journal of Applied Physiology 2003;19(4):344-409
Aging
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physiology
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Animals
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Catalase
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metabolism
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Circadian Rhythm
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Free Radicals
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metabolism
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Lipid Peroxides
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metabolism
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Male
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Melatonin
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metabolism
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Nitric Oxide
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metabolism
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Rats
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Rats, Sprague-Dawley
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Superoxide Dismutase
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metabolism
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Thymus Hormones
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pharmacology
6.Mining of antidepressant patents from Innography
Zhibin YANG ; Dong WANG ; Jin CHENG ; Weitao ZONG ; Wei LIU ; Lei WANG ; Yan ZHANG
Chinese Journal of Medical Library and Information Science 2015;(3):18-22
After the antidepressant patents were retrieved from Innography, their distribution, R& D level, hot spots and core patents were analyzed , which showed that the largest number of antidepressant patents was produced in USA, the medicinal preparations containing organic active ingredients were the hot spots, the technological and economic strengths of Pfizer, Novo and Sanofi-Aventis were relatively tremendous.The law-suited patents, reex-amined patents and patent strength were mined.
7.Experimental Study on Anaphylactoid Reactions of Hydroxyethyl Starch 40 Sodium Chloride Injection
Weifeng ZONG ; Yuxin WANG ; Yang LIU ; Yao WANG ; Weifeng ZHANG ; Yihong LU
Chinese Journal of Comparative Medicine 2017;27(1):64-66
Objective To evaluate the sensitizations of Hydroxyethyl Starch 40 Sodium Chloride Injection . Methods Active systemic anaphylaxis ( ASA) test, internal sensitive index ' s determination and screening and Passive Cutaneous Anaphylaxis ( PCA) test are carried out .Observe the allergy safety of the samples .Results We use guinea pigs to carry on ASA test and PCA test with Hydroxyethyl Starch 40 Sodium Chloride Injection from 45 batches of 3 companies and no changes have occurred .Measuring and comparing the sensitive index of HIS ,IgG, IgM and IgE in plasma, IgM and IgE are not obvious variation;HIS and IgG positive group and negative group and sample groups are different .Conclusions It is suggestion that Hydroxyethyl Starch 40 Sodium Chloride Injection safety evaluation should include allergic reactions , HIS and IgG sensitive index monitoring in clinical application of hydroxyethyl starch .
8.The significance of pro-gastrin-releasing peptide for small cell lung cancer diagnosis
Xing YANG ; Guirong SUN ; Peishan CONG ; Jinbao ZONG ; Haixia LI ; Mingjun LIU
Chinese Journal of Laboratory Medicine 2012;35(8):736-741
Objective To evaluate the clinical value of pro-gastrin-releasing peptide (ProGRP) for small cell lung cancer ( SCLC ).Methods Serum levels of ProGRP and neuron-specific enolase (NSE) were measured by both chemiluminescent immunoassay and electrochemiluminescent immunoassay in 46 patients with SCLC (26 patients with limited disease,20 patients with extensive disease ),51 patients with non-small cell lung cancer (NSCLC),45 patients with benign pulmonary diseases and 56 healthy subjects.Patients were recruited by the Affiliated Hospital of Medical College,Qingdao University,from September 2010 to April 2011.The receiver operating characteristic curves (ROC) was used to set the cutoff value of ProGRP and NSE and the areas under ROC ( ROC-AUC).The sensitivity and specificity of ProGRP and NSE were analyzed for diagnosing SCLC.Results Serum levels of ProGRP in healthy subjects,benign pulmonary diseases,NSCLC and SCLC groups were 22.9 ( 19.5 - 28.7 ),23.7 ( 20.0 - 27.8 ),28.9 (23.8-34.7) and 370.9( 129.4- 1951.6) ng/L respectively; the serum levels of NSE were 14.1 (12.5- 15.7),13.3(10.3- 15.3),16.8(11.7-22.1) and 39.9(16.1-93.9) μg/L,respectively.The Kruskal-Wallis H test showed significantly difference amoun groups of ProGRP and NSE (H =92.116 and 55.481,P <0.001 ).The serum levels of ProGRP in limited disease SCLC (LD-SCLC) group[ 156.2(65.4-547.5 ) ng/L]were also significantly higher than those in the healthy group,benign pulmonary diseases group and NSCLC group ( U =57,70 and 144,P < 0.001 ).In extensive disease SCLC (ED-SCLC) group,the ProGRP and NSE results[ 1933.1 (325.9 -4512.1) ng/L and 61.0(35.4- 115.5 ) μg/L ]were higher than those in the LD-SCLC group ProGRP,NSE [ 24.3 ( 15.1 - 16.3 ) μg/L,U =119 and 153,P < 0.05 ].Using healthy subjects group as control,the largest Youden index point of ROC was used to set the cut-off value of ProGRP and NSE (34.0 ng/L and 20.2 μg/L).The ROC-AUC of ProGRP (0.96 ) was statistically higher than that of NSE ( 0.86 ) in the SCLC group ( Z =2.57,P <0.05).The ROC-AUC results between combining detection of ProGRP and NSE (0.96 ) and ProGRP itself (0.96) were not significant difference ( Z =0.21,P > 0.05 ).The sensitivity of ProGRP ( 89.1% ) was statistically higher than that of NSE in the SCLC group (71.7%,x2 =4.90,P <0.05 ) ; the specificity of ProGRP (98.2%) compared with NSE did not have statistical significance (96.4%,x2 =0.00,P >0.05 ).The combining detection of ProGRP and NSE had no influence on the sensitivity and specificity compared with ProGRP itself (91.3% vs 89.1%,94.6% vs 98.2%,x2 were all 0.00,P > 0.05 ).Using benign pulmonary diseases group as control,the largest Youden index point of ROC was used to set the cutoff value of ProGRP and NSE (49.5 ng/L and 23.1 μg/L).The ROC-AUC of ProGRP (0.95) was statistically higher than that of NSE (0.87) in the SCLC group (Z =1.99,P <0.05 ).The ROC-AUC of combining detection of ProGRP and NSE ( 0.95 ) and ProGRP itself ( 0.95 ) were not difference significantly ( Z =0.02,P > 0.05 ).The sensitivity of ProGRP (84.8% ) was statistically higher than that of NSE in the SCLC group (69.6%,x2 =4.00,P <0.05);the specificity of it (97.8%) was equal to that of NSE (97.8%,x2 =0.50,P >0.05 ).The combining detection of ProGRP and NSE had no obviously influence on the sensitivity and specificity compared with ProGRP itself ( 87.0% vs 84.8%,95.6% vs 97.8%,x2 were all 0.00,P >0.05 ).Using NSCLC group as control,the largest Youden index point of ROC was to set the cut-off value of ProGRP and NSE (49.1 ng/L and 23.0 μg/L).The ROC-AUC of ProGRP ( 0.90) was statistically higher than that of NSE (0.76) in the SCLC group (Z=2.90,P<0.05).The ROC-AUC of combining detection of ProGRP and NSE (0.90 ) and ProGRP itself (0.90 ) were not difference significantly ( Z =0.00,P > 0.05 ).The sensitivity of ProGRP ( 84.8% ) was higher than that of NSE in the SCLC group ( 69.6%,x2 =4.00,P < 0.05 ) ; the specificity of it ( 96.1% ) was also higher than that of NSE (80.4%,x2 =6.13,P < 0.05 ).The combining detection of ProGRP and NSE had no obviously influence on the sensitivity and specificity compared with ProGRP itself ( 87.0% vs 84.8%,95.6% vs 96.1%,x2 were all 0.00,P > 0.05 ).Conclusion ProGRP has a higher diagnostic value than NSE in SCLC.
9.Evaluation of Clinical Detection Methods for Detection of Helicobacter Pylori Infection in Children
zong-ping, JIN ; yu-hua, LIU ; li, LI ; ai-jun, YANG
Journal of Applied Clinical Pediatrics 2006;0(19):-
Objective To explore the optimal methods to detect helicobacter pylori(Hp)antigen in children.Methods 13C labeled urea breath test(13C-UBT)was performed on 937 children of alimentary department from Sep.2000 to Feb.2006.Gastric mucosa biopsy of 96 children was detected.Hp stool antigen(HpSA)status of the 557 children were evaluated.Both 13C-UBT and HpSA were assayed in 105 children from Apr.2003 to Apr.2004.Method of 13C-UBT was taken as the golden standard on diagnosis of Hp infection.Results Forty-one point seven percent children was positive for 13C-UBT.Forty point six percent children was positive for gastric mucosa biopsy.Thirty-eight point two percent was positive for HpSA.The difference was not significant.2.Among the 105 children performed by both 13C-UBT and HpSA,41.9% was positive for 13C-UBT and 39.0% for HpSA.Taking 13C-UBT as the golden standard,sensitivity of HpSA to diagnose Hp infection was 91.8%,and its specificity was 81.8%.Consistency Kappa coefficient was 0.743(P=0),which denoted that there was no significant difference on the positive detection rate between 13C-UBT and HpSA(P=0.388).Conclusions 13C-UBT and HpSA as non-invasive technique is effective to detect the Hp antigen.Compared to 13C-UBT,as a convenient,noninvasive,economical method,HpSA detection is much more acceptable to children and their patients.
10.Outcome assessment of health education on endemic diseases in Henan province in 2010
Yang, LIU ; Xiao-feng, LI ; Yi-tian, YUE ; He-ming, ZHENG ; Bo, YU ; Hong-yang, YU ; Zong-yu, HAO
Chinese Journal of Endemiology 2013;(1):104-108
Objective To evaluate the effect of health education on endemic diseases in Henan province in 2010.Methods According to the requirements of The Education Program for Endemic Diseases Control in 2009 Henan Province,50 counties (districts) of 18 cities with endemic diseases were selected.Referring to the health education questionnaire in the program,knowledge of endemic diseases were asked of pupils and housewives.Intervention effects were evaluated two months after teaching pupils and housewives in counties,towns,villages and schools with the knowledge of endemic diseases and the questionnaire was used.Results Among 50 counties investigated,5523 pupils and 3206 housewives were surveyed in the baseline according to the requirement,and 5417 pupils and 2891 housewives were surveyed for outcome evaluation.The results showed that after this education,pupils' knowledge about endemic diseases increased from 66.31%(10 987/16 569) in the baseline survey to 93.84%(15 250/16 251),the difference have statistical significant(x2 =3877.78,P < 0.01) ; housewives from 67.56%(6133/9078)to 92.67%(8037/8673),the difference have statistical significant(x2 =1736.33,P < 0.01).After the education,the pupils' knowledge about the iodine deficiency disorders,drinking-water-borne fluorosis and arsenic poisning,coal-burning-borne fluorosis increased from 70.15% (6263/8928),62.29%(4423/7101),42.96% (185/270),68.52% (116/270) to 94.19% (8344/8859),93.05% (6376/6852),99.63% (261/270),96.67% (269/270),the difference have statistical significant (x2 =1749.85,1939.26,211.83,74.43,all P < 0.01),housewives' knowledge about the iodine shortage diseases,drinking-water-borne fluorosis,the endemic fluorine,the arsenic poisoning increased from 73.27% (3330/4545),62.79% (2677/4263,30.37% (85/135),62.96%(41/135) to 93.96%(4228/4500),91.08%(3555/3903),97.04%(123/135),91.11%(131/135),the difference have statistical significant (x2 =751.03,924.65,129.75,30.23,all P < 0.01).Conclusions Health education intervention has a marked effect in increasing the peoples' knowledge of endemic diseases.Therefore health education should be enhanced in the future.