1.Effect of soluble CD44 molecule on the expression of apoptosis regulatory protein bcl-2 associated death factor bad in human trabecular meshwork cell
Zong-bao, LIANG ; Yu-yu, WU ; Mao-sheng, GUO
Chinese Journal of Experimental Ophthalmology 2012;30(3):224-227
BackgroundResearches demonstrated that the levels of soluble CD44 (sCD44)molecule in aqueous is significantly higher in primary open-angle glaucomous(POAG) eye than normal eye,but how the sCD44 would affect the expression of apoptosis protein in trabecular meshwork cells is below understanding.Objective The present study was to investigate the effect of sCD44 on the expression of regulatory proteins bcl-2 associated death factor bad in trabecular meshwork cells in the patients with POAG.MethodsHuman scleral tissue with trabecular meshwork were obtained from POAG patients during the surgery.The trabecular meshwork cells were primarily cultured by explant culture method and identified by immunochemistry.The third generation of cells were incubated with free-serum DMEM/F12 medium added differnt dosages of sCD44 (0,1,5,10,25,50 mg/L) for 48 hours.The expression of bad protein in cultured cells was detected using cell counting kit-8 (CCK-8) as the absorbance values at 490 nm(A,90 value),and the bad protein level in cultured cells was assayed by ELISA.ResultsThe cultured cells showed the positive response for laminin ( LM ),neuron specific enolase ( NSE ),fibronectin ( FN ) monoclonal antibodies.The CCK-8 assay showed that the A490 values of the trabecular meshwork cells in 0,1,5,10,25,50 μg/L of sCD44 groups were 0.2460±0.0019,0.1874±0.0015,0.1570±0.0016,0.1302±0.0019,0.1084±0.0018,0.0940±0.0020 respectively with a statistically significant difference among the 6 groups( F =14.922,P =0.000 ),and the A490 values in various dosages of sCD44 groups were significantly lower than the 0 μg/L sCD44 group (P=0.013,0.008,0.011,0.005,0.004).The ELISA assay showed that bad protein levels in 0,1,5,10,25,50 μg/L of sCD44 groups were ( 114.8461 ± 2.9560 ),( 137.8270 ± 2.4259 ),( 161.4194 ± 3.7381 ),( 170.9453 ± 3.2006 ),( 221.2252 ±4.3738 ),( 324.6167±4.4220) ng/L,showing a total difference among them ( F =16.610,P =0.000 ),and the bad protein levels in various dosages of sCD44 groups were significantly lower than the 0 μg/L sCD44 group( P =0.017,0.013,0.008,0.007,0.006).ConclusionssCD44 can contribute to the apoptosis of the trabecular meshwork cells in patients with POAG in certain dose range by regulating the apoptosis regulatory proteins bcl-2 associated death factor bad.
2.Practical issues on histopathologic diagnosis of nasopharyngeal carcinoma.
Yong-sheng ZONG ; An-jia HAN ; Qiu-liang WU
Chinese Journal of Pathology 2010;39(8):566-569
Biopsy
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Carcinoma, Squamous Cell
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classification
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metabolism
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pathology
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Epithelial-Mesenchymal Transition
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Humans
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Immunohistochemistry
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Nasopharyngeal Neoplasms
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classification
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metabolism
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pathology
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Nasopharynx
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pathology
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RNA, Viral
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metabolism
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Tumor Suppressor Protein p53
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metabolism
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Viral Matrix Proteins
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metabolism
4.Nasopharyngeal manifestations and outcomes of acute dimethyl sulfate poisoning.
Jian WU ; Zhu-sheng CHEN ; Zong-hui KANG ; Lian-mei YE ; Lei YANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(2):115-116
Adolescent
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Adult
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Environmental Exposure
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Female
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Humans
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Inhalation Exposure
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Male
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Middle Aged
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Nasopharynx
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pathology
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Sulfuric Acid Esters
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poisoning
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Young Adult
5.Sinonasal primary extramedullary solitary plasmacytoma with Epstein-Barr virus infection: report of a case.
Yan-fen FENG ; Qiu-liang WU ; Yong-sheng ZONG ; Qiong SHAO
Chinese Journal of Pathology 2007;36(10):711-712
Antibodies, Monoclonal
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metabolism
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CD79 Antigens
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metabolism
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Epstein-Barr Virus Infections
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Herpesvirus 4, Human
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isolation & purification
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Humans
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Immunoglobulin G
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metabolism
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Male
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Middle Aged
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Nose Neoplasms
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metabolism
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pathology
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therapy
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virology
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Paranasal Sinus Neoplasms
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metabolism
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pathology
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therapy
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virology
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Plasmacytoma
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metabolism
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pathology
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therapy
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virology
6.Isolation,incubation and identification of parenchymal neural stem cells in adult mouse spinal cord
Hui ZHANG ; Zong-Sheng YIN ; Sheng-Quan ZHANG ; Wei WANG ; Xing-Yi HUA ; Yong HU ; Guang-Wu LI ;
Chinese Journal of Trauma 2003;0(07):-
Objective To isolate and identify the adult neural stem cells from the parenchyma of spinal cord in adult mouse.Methods The parenchymal spinal cord from adult mouse was dissected and dissociated by mechanical trituration.The tissue suspension was cultured in serum-free DMEM/F12 medium supplemented with EGF and B27.The cell colonies generated from a single cell were screened by limited dilution and incubated with BrdU.The cell colonies were transferred into medium with serum to induce differentiation.The cells were identified with antibodies to Nestin,BrdU,MAP2 and GFAP by immunofluorescence staining.Results The cells were cultured for seven days to generate proliferative neurospheres.The majority of cells in these neurospheres expressed Nestin and were differentiated into MAP2-positive cells and GFAP-positive cells in medium containing with fetal bovine serum.Conclusion A significant number of neural stem cells are present in the parenchymal adult mouse spinal cord and can proliferate and also give rise to neurons and glia in vitro.
7.Stady of the nitric oxide,oxygen free radical in serun and intestine mucosal in rats
Jian-Sheng LIU ; Yi TIAN ; Xiao-Hong ZHANG ; Hai-Ling LIU ; Jing SUN ; Yao-Zong YUAN ; Yun-Lin WU ;
Chinese Journal of Primary Medicine and Pharmacy 2005;0(11):-
Objective To explore the changes of nitric oxide(NO) and superoxide dismutase(SOD) in the serun and intestine mucosal and the treatment of ulcerative colitis(UC) with tanshinon in rats,Methods 30 mg of trinitro-benzene-sulfonic acid (TNBS) dissolved in 0.85ml of 50% ethanol was administrated intrarectally in Sprague-Dawley female rats to induce experimental colitis.After 7 days,the rats were divided into normal control, 0.9% saline and treatment group tanshinon 2ml?kg~(-1)?d~(-1) were intravenously.The therapeutic effect was evaluated by measuring the ponderal index,the surface area of the ulcers,macroscopical and histological score,activity of NO and SOD were measured in colonic tissue and serum all rats.Results Compared with the saline group,the ponderal index,the surface area of the ulcers,macroscopical and histological score,activity of NO level in the serum and intes- tine mucosal was decreased and the SOD increased of significantly in the treatment group(P
8.Clinical assessment on treatment of hyperlipidemia with pushen capsule.
Zong-lian LIU ; Sheng-xian WU ; Gai-di GAO
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(3):227-229
OBJECTIVETo explore the therapeutic effect of Pushen capsule (PSC) in treating primary hyperlipidemia.
METHODSTwo hundred and forty patients with primary hyperlipidemia were randomly divided into two groups, the 120 patients in the treated group treated with PSC (4 capsules, tid) and the 120 patients in the control group treated with Zhibituo tablet (3 tablets, tid), and they were administered at the same time with Zhibituo placebo. The therapeutic course for both groups was 4 weeks. The therapeutic effect and the effects on blood lipids and viscosity were observed.
RESULTSThe effective rate in the treated group was 76.3%, which was significantly higher than that in the control group (48.7%, P < 0.01). PSC showed a significant lowering effect on TC, TG and LDL-C and raising effect on HDL-C, and the effect in lowering TG was significantly better than that of Zhibituo (P < 0.01). PSC also showed a certain effect in decreasing whole blood viscosity of both high-sheared and low-sheared viscosity.
CONCLUSIONPushen capsule has promising blood lipid regulating effect in patients with hyperlipidemia, and some effects in lowering the blood viscosity.
Adult ; Aged ; Blood Viscosity ; Capsules ; Cholesterol, HDL ; blood ; Cholesterol, LDL ; blood ; Double-Blind Method ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Hyperlipidemias ; drug therapy ; Hypolipidemic Agents ; therapeutic use ; Male ; Middle Aged ; Phytotherapy ; Prospective Studies ; Triglycerides ; blood
9.Changes in the mRNA expression of adiponectin, adiponectin receptors, and leptin in adipose tissue of Wannanhua pigs at different stages of development.
Sheng SHENG ; Jie ZHOU ; Jia ZHANG ; Kang SHAO ; Xiao-Xue WU ; Wei-Xin LI ; Zong-Jun YIN
Chinese Journal of Applied Physiology 2012;28(4):352-355
OBJECTIVETo explore the changes in the mRNA expression of adiponectin (Adp), adiponectin receptors(AdpR), and leptin in different adipose tissues of Wannanhua pigs at different stages of development, and their sexual dimorphism.
METHODSFive Wannanhua boars and five Wannanhua gilts were sampled at birth, 30, 45, 90, and 180 days of age respectively. The delta delta Ct relative quantification real-time PCR was used to detect the transcription levels of Adp, AdpR1, AdpR2, and leptin mRNAs in subcutaneous (SC) and perirenal (PR) adipose tissues, and beta-actin were used as internal standards.
RESULTSThe expression level of Adp, AdpR1, AdpR2, and leptin mRNA in SC and PR adipose tissue were changed with age significantly (P < 0.01). In general, Adp mRNA expression in SC adipose tissue was significantly lower than that in PR adipose tissue (P < 0.05), while AdpR1, AdpR2, and leptin mRNA expression in SC adipose tissue were significantly higher than those in PR adipose tissue (P < 0.05 or P < 0.01). Although the sexual dimorphism were found in apart genes or apart days of age, Adp, AdpR1, AdpR2, and leptin mRNA expression both in SC adipose tissue and PR adipose tissue had no significant differences between Wannanhua gilts and boars in general. Significant positive correlation was found between Adp and AdpR1, AdpR2 (P < 0.05 or P < 0.01), and significant negative correlation was found between Adp and leptin (P < 0.05) in SC adipose tissue and PR adipose tissue respectively (P < 0.05).
CONCLUSIONThe expression of Adp, AdpR1, AdpR2, and leptin mRNA in adipose tissue of Wannanhua pigs followed specific developmental patterns and tissue specificity. Adp correlated with its receptors.
Actins ; metabolism ; Adiponectin ; metabolism ; Adipose Tissue ; growth & development ; metabolism ; Animals ; Female ; Leptin ; metabolism ; Male ; RNA, Messenger ; genetics ; Receptors, Adiponectin ; metabolism ; Swine
10.Study on moxa sticks burning temperature-time-space curves.
Zong-Guo HONG ; Feng LÜ ; Hai-Sheng WEI ; Yu-Hong YUAN ; Huan-Gan WU
Chinese Acupuncture & Moxibustion 2012;32(11):1024-1028
OBJECTIVETo study the burning characteristics of moxa stick.
METHODSA self-designed moxa stick burning temperature measuring device, which was assembled with ALTEC intelligence digital setter and SJ-600 thermocouple, was used to conduct next four experiences: 1) embedding a thermocouple inside a moxa stick to measure peak burning temperature; 2) pulling a thermocouple embedded in the moxa stick at the proper rate to detect combustion stability; 3) elucidating temperature distribution of transverse section by measuring the temperature in the center, radius midpoint and lateral; 4) drawing temperature-time-space curves by pulling the thermocouples in the former three observation points.
RESULTSThe experiment indicated that the burning temperature peak of three-year moxa stick (Hubei Herbal Medicine St. Qichun Technology Co., Ltd.) was 848 degrees C which had good combustion stability. Furthermore, the temperature in the center, radius midpoint and lateral of transverse section were 843 degrees C, 731 degrees C and 410 degrees C, respectively, and its burning temperature-time-space curves was drawn, which showed the real-time burning temperature and the peak burning temperature and were regarded as ultimate indice to integrate the formers.
CONCLUSIONThe measuring system elaborately reflecting the burning features of moxa stick may provide reference for manufacture industry of moxa stick quality criteria for its convenience and accuracy.
Humans ; Moxibustion ; instrumentation ; Temperature ; Time Factors