2.Overview of algorithm for diagnosis and treatment of chronic cough in children: Intensive reading of the guideline for diagnosis and treatment of chronic cough in pediatrics (I).
Chinese Journal of Pediatrics 2008;46(10):755-756
Algorithms
;
Child
;
Chronic Disease
;
Cough
;
diagnosis
;
therapy
;
Guidelines as Topic
;
Humans
7.Significance of Expression of Interferon ? Inducible Protein 10 in Peripheral Blood Mononuclear Cells in Children with Kawasaki Disease
de-sheng, JIA ; ji-min, SUN ; zong-qi, DONG
Journal of Applied Clinical Pediatrics 2006;0(19):-
0.05).Conclusions The expression of PBMC IP-10 mRNA increases in KD.IP-10 may participate in the pathogenesis of KD and CAL in KD.Perhaps the inhibition to the expression of PBMC IP-10 mRNA is one of the mechanisms of IVIG.
8.Research Progress About Some Problems on Cordyceps militaris
Yan-Feng HAN ; Jian-Dong LIANG ; Wen DU ; Zong-Qi LIANG ;
Microbiology 2008;0(09):-
Cordyceps militaris is of significant economic value in medical care and food exploitation because of its many physiological activities. This paper reviews(1) the taxonomic position of its anamorph,(2) interesting culture ways,(3) strain degeneration and genetic variability,and(4) research progress in bioactive compounds and pharmacological functions.
9.Enhanced effect of guizhi plus Gegen Decoction on learning and memory disorder in LPS induced neuroinflammatory mice.
Ying XU ; Zong-Qi ZHANG ; Yan ZHAO ; Xian-Wen DONG ; Xing-Yu WANG ; Li-Qing ZHANG
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(2):179-184
OBJECTIVETo explore the potential effect of Guizhi plus Gegen Decoction (GGD) in improving learning and memory of lipopolysaccharides (LPS) induced neuroinflammatory mice and its possible mechanisms.
METHODSTotally 63 male ICR mice were randomly divided into 5 groups, i.e., the normal control (n = 13), the model group (n = 13), the low dose GGD group (n = 10), the high dose GGD group (n = 14), and the positive control group (n = 13). Mice were intraperitoneally injected with LPS (0.33 mg/kg) to induce Alzheimer's disease (AD) model. Mice in the high and the low dose GGD groups were administered with 12 g/kg or 6 g/kg by gastrogavage for 4 successive weeks. Mice in the control group were intraperitoneally injected with minocycline (50 mg/kg) for 3 days. By the end of treatment LPS were injected 4 h before behavior test each day, and then behavior test was conducted in mice of each group. Effect of GGD on learning and memory of AD mice was observed by using open field test, novel object recognition task, and Morris water maze.
RESULTSOpen field test showed there was no statistical difference in the movement time and the movement distance among all groups (P > 0.05), suggesting that LPS and GGD had no effect on locomotor activities of mice. In novel object recognition test, AD mice spent significantly shorter time to explore novel object after they were induced by LPS (P < 0.05), while for AD mice in the low and high dose GGD groups, their capacities for exploration and memory were significantly improved (P < 0. 05, P < 0.01). Results of Morris water maze showed that AD mice exhibited increased escape latency (P < 0.05) and spent much less time in swimming across the original platform (both P < 0.05). However, AD mice in the low and high dose GGD groups had obvious shortened latency and increased time percentage for swimming (P < 0.05, P < 0.01).
CONCLUSIONGGD possessed certain improvement in learning and memory disorder of LPS induced AD mice.
Alzheimer Disease ; chemically induced ; drug therapy ; psychology ; Animals ; Drugs, Chinese Herbal ; therapeutic use ; Lipopolysaccharides ; adverse effects ; Male ; Memory Disorders ; prevention & control ; Mice ; Mice, Inbred ICR ; Neuritis ; chemically induced ; drug therapy ; psychology ; Phytotherapy
10.Small hairpin RNA silencing Tim-3 affects peripheral blood Th1 and Th17 cells differentiation in asthmatic mice.
Xiao-Xia LU ; Jia-Li XU ; Zong-Qi DONG ; Peng CHEN ; Ying WANG
Chinese Journal of Contemporary Pediatrics 2013;15(4):302-307
OBJECTIVETo investigate the effects of down-regulating Tim-3 gene in the peripheral blood mononuclear cells (PBMCs) of an asthmatic mouse model by short hairpin RNA (shRNA) and to explore the effect of Tim-3 on Th1 and Th17 cell differentiation.
METHODSAn asthmatic murine model was established by ovalbumin sensitization and challenge. PBMCs were isolated from asthmatic mice and transfected by shRNA targeting Tim-3 gene. The mRNA and protein expressions of Tim-3 were detected by quantitative PCR and Western blot. Flow cytometry analysis was performed to determine the levels of Th1 and Th17, and ELISA was performed to determine concentrations of IFN-γ, IL-4 and IL-17 in the supernatant.
RESULTSTim-3 mRNA expression in PBMCs was significantly increased in asthmatic mice. The mRNA and protein expression of Tim-3 decreased significantly in the shRNA group. Compared with the negative groups, Th1 cell levels increased and Th17 cell levels decreased significantly in the asthmatic groups after Tim-3 shRNA interference. In the Tim-3 shRNA interference groups concentrations of IFN-γ increased significantly while IL-17 decreased significantly.
CONCLUSIONSSpecific Tim-3 shRNA effectively silences the expression of Tim-3 and change in Tim-3 expression could affect T cell differentiation.
Animals ; Asthma ; immunology ; therapy ; Cell Differentiation ; Female ; Gene Silencing ; Hepatitis A Virus Cellular Receptor 2 ; Mice ; Mice, Inbred BALB C ; RNA, Small Interfering ; genetics ; Receptors, Virus ; antagonists & inhibitors ; genetics ; Th1 Cells ; cytology ; Th17 Cells ; cytology