1.Lipid Production by Rhodosporidium toruloides Using Jerusalem Artichoke Tubers
Yan-Yan HUA ; Xin ZHAO ; Jin ZHAO ; Su-Fang ZHANG ; Zong-Bao ZHAO ;
China Biotechnology 2006;0(10):-
The effects of pretreatment methods of Jerusalem artichoke tubers on microbial lipids fermentation with an oleaginous yeast strain Rhodosporidium toruloides Y4 were investigated in shaking flask culture.The yeast strain accumulated substantial amount of lipids using either purple-or white-skinned Jerusalem artichoke tubers as sole carbon and energy source.When cells were cultured on the extracted juice or the acidhydrolysate,cellular lipid content reached 40%(w/w),while cultured on the pulp,the white-skinned tubershadhigher lipid productivity,yielding 12.1 g lipids per100 g dried tubers.Major fatty acid constituents of microbial lipids were those contained 16-and 18-carbon atoms based on GC analysis,which is quite similar to traditional vegetable oil.Microbial lipids prepared from Jerusalem artichoke can be applied to biodiesel production.
2.Optimized culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides.
Yong-Hong LI ; Bo LIU ; Zong-Bao ZHAO ; Feng-Wu BAI
Chinese Journal of Biotechnology 2006;22(4):650-656
Culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides were optimized with single factor and uniform design experiment. The best medium recipe was found with 70 g/L glucose, 0.1 g/L (NH4)2SO4, 0.75 g/L yeast extract, 1.5 g/L MgSO4. 7H2O, 0.4g/L KH2PO4, sterilized at 121 degrees C for 15 min, and then supplemented with ZnSO4 1.91 x 10(-6) mmol/L, CaCl2 1.50 mmol/L, MnCl2 1.22 x 10(-4) mmol/L and CuSO4 1.00 x 10(-4) mmol/L. The optimal fermentation conditions were as follows: 50 mL of medium (pH 6.0) in 250 mL Erlenmeyer flask with 10% inoculum (28h) under orbital shaking at 200 r/min for 120h at 30 degrees C. Under these conditions, yeast biomass accumulated lipids up to 76.1%.
Basidiomycota
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growth & development
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metabolism
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Copper
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pharmacology
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Culture Media
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Fermentation
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Hydrogen-Ion Concentration
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Lipids
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biosynthesis
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Magnesium Sulfate
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pharmacology
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Zinc
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pharmacology
3.Primers for detecting gene rearrangement in different regions of immunoglobulin heavy chain genes and their application in diagnosis of paraffin-embedded lymphoma tissues.
Zong-Li QI ; Bao ZHANG ; Xi-Qun HAN ; Mei-Gang ZHU ; Tong ZHAO
Journal of Southern Medical University 2008;28(11):1964-1967
OBJECTIVETo analyze and optimize the gene rearrangement primers of different frame regions (FR) of immunoglobulin heavy chain (IgH) genes by bioinformatic methods and explore the application of these primers in the detection of paraffin-embedded lymphoma tissues.
METHODSThree pairs of primers from IgH FR1, FR2 and FR3 regions (P1c, P2A and P31, respectively) were selected as the B cell gene rearrangement primers after comparison of the gene fragments in 44 IgH variable and 6 joining regions. Using one pair of T cell receptor (TCR) gamma primer as the T cell gene rearrangement primer, 101 histopathologically confirmed lymphoproliferative samples including 80 B cell lymphomas, 14 T cell lymphomas, and 7 reactive proliferative lymph nodes were examined by PCR for gene arrangement. The DNAs from DG75 and Jurkat cell lines were used as the positive controls for B and T cell lymphoma, respectively, with those from reactive proliferative lymph nodes as the negative control.
RESULTSThe positivity rates of IgH primers (P1c, P2A and P31) in the 80 B cell lymphomas were 37.5% (30/80), 52.5% (42/80) and 70.0% (56/80), respectively, and only one of the 14 T cell lymphoma cases was positive for the primers, suggesting significant differences in the detection rates of B cell lymphomas by the 3 primers. The detection rate was increased to 83.9% by combining the results by P31 and P2A primers. No positivity was found in the proliferative reaction tissues.
CONCLUSIONPrimers from IgH FR3 region genes are more sensitive than that from the FR1 and FR2 regions in the detection of gene rearrangement in paraffin-embedded lymphoma tissues. The detection rates can be increased by combining the results with the primers for IgH FR3 with that of FR2.
DNA Primers ; Gene Rearrangement, B-Lymphocyte, Heavy Chain ; genetics ; Humans ; Immunoglobulin Heavy Chains ; genetics ; Lymphoma, B-Cell ; diagnosis ; genetics ; pathology ; Lymphoma, Non-Hodgkin ; diagnosis ; genetics ; pathology ; Lymphoma, T-Cell ; diagnosis ; genetics ; pathology ; Male ; Paraffin Embedding
4.Different sources of mesenchymal stem cells for the treatment of cartilage repair in knee joint.
Lian WANG ; Peng HOU ; Tao JIANG ; Zong-Bao WANG ; Yin-Xue ZHAO ; Ke WU
China Journal of Orthopaedics and Traumatology 2017;30(6):581-586
As propose of organ repair stem cell therapy technology, articular cartilage cannot be repaired by itself has become one of the research hotspots, repair of articular cartilage with mesenchymal stem cells has shown obvious advantages for the treatment. The scholars have made a preliminary study on the role of mesenchymal stem cells from different sources in the repair of knee articular cartilage, and with the combination of transplantation and cartilage tissue engineering, these technologies improved the human cartilage repair effect of bone marrow, adipose, synovium, cord blood derived stem cells, which achieved good clinical curative effect. Due to the different sources, the dominant and recessive factors, each stem cell will have certain advantages and disadvantages. At present, the clinical research is still in the experimental stage, there is no definite conclusion on which kind of stem cell or technology is more suitable for human cartilage repair. This requires the validation of large-scale or combining with new processing technology clinical trials and the long-term clinical effect, it also provides for the basis for further clinical research.
5.Anatomic study and clinical application of thinned posterior tibial artery perforator flap.
Feng-Lin ZHAO ; Zong-Bao LI ; Xin WANG ; Hui-Feng HAN ; Chang-De WANG ; Liang HU
Chinese Journal of Plastic Surgery 2013;29(4):261-265
OBJECTIVETo explore the feasibility and therapeutic effect of thinned posterior tibial artery free perforator flap for the reconstruction of soft tissue defects at dorsum of hands.
METHODSSix fresh adult lower limbs specimens were injected with red latex via arterial cannula and dissected. The number, distribution, branches, and outer diameter of posterior tibia artery perforators were observed. Based on the anatomic study, the perforator flaps were designed to reconstruct soft tissue defects at dorsum of hands and wrists. The redundant fat on the flaps was removed, but preserving the nutrient vascular system. 11 flaps were used with the size ranging from 2 cm x 5 cm to 10 cm x 14 cm.
RESULTS43 skin perforators of posterior tibial artery were observed in six lower limbs, 29 perforators with the outer diameter is greater than 0.5 mm when they threading over the deep fascia plane, on average every 4.8 bundles of sides. The mean outside diameter of perforating artery is (1.8 +/- 0.5) mm, and the length is (44 +/- 15) mm. 6 perforators were founded both in the second and fifth zone which could be used for anastomosis for its better diameters. All flaps survived completely without any complication at donor sites. 7 cases were followed up for 3-12 months. Both satisfactory functional and cosmetic results were achieved with a soft and thinned appearance.
CONCLUSIONSThe thinned posterior tibial artery free perforator flap has a reliable blood supply and good appearance. It is very suitable for the reconstruction of small or medium-sized defects at the dorsum of hands and wrists.
Adolescent ; Adult ; Female ; Hand Injuries ; surgery ; Humans ; Lower Extremity ; anatomy & histology ; blood supply ; Male ; Middle Aged ; Perforator Flap ; blood supply ; Reconstructive Surgical Procedures ; methods ; Tibial Arteries ; anatomy & histology ; transplantation ; Young Adult
6.Correlation between APOA gene polymorphism and intracranial aneurysm.
Yuan-li ZHAO ; Yu-ming SUN ; Shuo WANG ; Ji-zong ZHAO ; Bao-sheng CHEN
Acta Academiae Medicinae Sinicae 2005;27(1):31-34
OBJECTIVETo study the correlation between a pentanucleotide repeats (PNR) polymorphism of APOA and the genesis of intracranial aneurysm.
METHODSFifty-eight patients with intracranial aneurysms diagnosed by angiography and 58 healthy controls were enrolled in our study. The gene polymorphism of APOA PNR were detected by polymerase chain reaction (PCR) and non-denatured polyacrylamide gels electropherosis (PAGE).
RESULTSEight kinds of gene types and 6 kinds of alleles were found in these two groups. There were two sites of sequence variance in the 5' control region of APOA gene, which were significantly different between the patients and controls.
CONCLUSIONCorrelation may exist between intracranial aneurysm and APOA gene.
Adult ; Aged ; Alleles ; Apolipoproteins A ; genetics ; Asian Continental Ancestry Group ; DNA ; genetics ; Female ; Gene Frequency ; Genotype ; Humans ; Intracranial Aneurysm ; genetics ; Male ; Microsatellite Repeats ; Middle Aged ; Polymorphism, Genetic
7.Effect of curved cutter stapler in the ultra low anterior resection for low rectal cancer.
Fang-Hai HAN ; Zhao-da ZHANG ; Zong-Guang ZHOU ; Xu-Bao LIU ; Wei-Ming HU ; Wei JIA ; Xiang ZHOU ; Hui-Min LU
Chinese Journal of Gastrointestinal Surgery 2007;10(1):60-63
OBJECTIVETo evaluate the curative effect of curved cutter stapler (Contour, Ethicon Endo-Surgery, Inc) in the ultra low anterior resection for low rectal cancer.
METHODSClinic data of 56 patients with low rectal cancer from Dec. 2005 to Sep. 2006 were reviewed retrospectively. After total mesorectal excision (TME) and lateral lymph node dissection (LLD) in 56 cases, the rectal (anal) remnant was cut and closed with curved cutter stapler (Contour), and preserved for ultra low colo-rectal (anal) anastomoses with 33 mm straight intraluminal stapler.
RESULTSThere was no operational death and the mean hospitalization time was (11.2+/-3.2) days. The incidence rate of postoperative complications in 1 month was 3.57% (2/65). Both of the cases were anastomotic leakage. One was cured by surgical drainage, the other combining with rectal vaginal fistula was cured by transverse colostomy.
CONCLUSIONCurved cutter stapler has the advantages of complete cutting, safe closure and low anastomotic leakage rate in the process of ultra low anterior resection for low rectal cancer.
Adult ; Aged ; Anastomosis, Surgical ; instrumentation ; methods ; Female ; Humans ; Middle Aged ; Rectal Neoplasms ; pathology ; surgery ; Rectum ; pathology ; Retrospective Studies ; Surgical Equipment
8.Microcalorimetric investigation of two cephalosporins on colon bacteria activity.
Fen XU ; Cheng-Gong SONG ; Rui-Hua WU ; Li-Ni YANG ; Li-Xian SUN ; Zong-Bao ZHAO ; Zhi-Heng ZHANG ; Zhong CAO ; Ling ZHANG
Acta Pharmaceutica Sinica 2009;44(10):1127-1130
The effects of cephradinum and ceftazidime on the metabolism of Escherichia coli (E. coli) DH5alpha was determined by microcalorimetry. The microbial activity was recorded as power-time curves through an ampoule method with a TAM Air Isothermal Microcalorimeter at 37 degrees C. The parameters such as the growth rate constant (k), inhibitory ratio (I), the maximum power output (Pm) and the time (tm) corresponding to the maximum power output were calculated. The results show that the ceftazidime has a better inhibitory effect on E. coli DH5alpha than cephradinum.
Anti-Bacterial Agents
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administration & dosage
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pharmacology
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Calorimetry
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methods
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Ceftazidime
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administration & dosage
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pharmacology
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Cephradine
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administration & dosage
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pharmacology
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Escherichia coli
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drug effects
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growth & development
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metabolism
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Microbial Sensitivity Tests
9.The effect of intraoperative continuous nimodipine infusion on cerebral vasospasm during intracranial aneurysm surgery.
Ru-quan HAN ; Bao-guo WANG ; Shu-ren LI ; En-zhen WANG ; Wei LIU ; Shuo WANG ; Ji-zong ZHAO
Chinese Journal of Surgery 2004;42(24):1489-1492
OBJECTIVETo evaluate the effect of intraoperative continuous nimodipine infusion on cerebral vasospasm during intracranial aneurysm surgery.
METHODSThirty consecutive patients under-going intracranial aneurysmal surgery were prospectively randomized into two groups: Isoflurane (group A, n = 15) and nimodipine (group B, n = 15). The patients in group A were maintained with 1 minimum alveolar concentration (MAC) isoflurane anesthesia during the whole procedure. The patients in group B were given nimodipine infusion continuously (20 microg.kg(-1).h(-1)) after induction of anesthesia and anesthetized with 1 MAC isoflurane. S100B levels in cerebrospinal fluid were determined before aneurysm clipping and 0, 2, 4 h after aneurysm clipping by enzyme linked immunosorbent assay. Assessment of mean blood flow velocity of parent arterial and arterial branches were performed before and after aneurysm clipping.
RESULTS(1) S100B in cerebrospinal fluid was increased significantly at 4 h after aneurysm was clipped in group A (F = 4.11, P < 0.05). However, S100B in cerebrospinal fluid was stable in group B in the whole procedure. (2) Mean arterial flow velocity of parent vessels in group B was lower significantly than that in group A (t = 2.08, P < 0.05). However, mean arterial flow velocity of distal vessels in both groups has no significant difference.
CONCLUSIONIntraoperative nimodipine infusion may prevent cerebral vasospasm during intracranial aneurysm surgery.
Adult ; Aged ; Anesthesia, Inhalation ; Anesthesia, Intravenous ; Anesthetics, Intravenous ; administration & dosage ; Female ; Humans ; Intracranial Aneurysm ; surgery ; Intraoperative Complications ; prevention & control ; Isoflurane ; administration & dosage ; Male ; Middle Aged ; Nimodipine ; therapeutic use ; Vasodilator Agents ; therapeutic use ; Vasospasm, Intracranial ; prevention & control
10.China coronary secondary prevention study (CCSPS): outcomes from analysis of coronary heart disease patients with diabetes.
Zong-liang LU ; Bao-min DU ; Zuo CHEN ; Yang-feng WU ; Xue-hai YU ; Yu-chen ZHAO ; null
Chinese Journal of Cardiology 2005;33(12):1067-1070
OBJECTIVETo elucidate whether lipid-lowering therapy with Xuezhikang can induce a decrease of cardiac events and an attenuation of total mortality in coronary heart disease (CHD) patients with diabetes.
METHODSWe designed a random, double-blinded, placebo controlled clinical trial in selected 591 patients. All patients were administrated with capsule Xuezhikang (0.6 g, Bid) or placebo in addition to conventional therapy. The mean follow-up period was four years. The primary end-points were nonfatal myocardial infarction and death from CHD.
RESULTS(1) The incidence of CHD events and that of death from CHD were reduced by 50.8% (P = 0.0008) and by 44.1% (P = 0.0246) in treatment group, respectively; Also, the incidence of nonfatal myocardial infarction was reduced by 63.8% (P = 0.0151). (2) The incidence of stroke, tumor, and PCI/CABG were decreased by 20.2%. (3) The total mortality were lowered by 44.1% in treatment group (P = 0.0097).
CONCLUSIONXuezhikang can effectively reduce the incidence of cardiac events and total mortality in CHD patients with diabetes.
Aged ; China ; Coronary Disease ; complications ; drug therapy ; prevention & control ; Diabetes Mellitus ; Double-Blind Method ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Follow-Up Studies ; Humans ; Hypolipidemic Agents ; therapeutic use ; Male ; Middle Aged ; Secondary Prevention