Objective To explore the diagnostic significance of C reactive protein(CRP)in diagnosing haemorrhagic fever with renal syndrome(HFRS).Methods 96 cases of patients with HFRS of different stages were enrolled in this study,and serum speci-men were collected.30 cases of patients with fever of unknown origin(fever of unknown origin group)and and 30 healthy individu-als(healthy control)were selected as control.Serum levels of CRP,alanine aminotransferase(ALT),aspartate amino-transferase (AST),creatine phosphokinase(CK),lactate dehydrogenase(LDH)and creatinine(Cr)were detected.Results Serum levels of CRP in patients with HFRS of different stages were lower than that in the fever of unknown origin group,had statistically significant differences(P <0.05).The variation trend of CRP in each stage of HFRS was consistent with the trend of AST,CK,LDH and Cr. Conclusion CRP has clinical significance in differentiating HFRS from fever of unknown origin.

Objective To study the changes condition and clinical significance of serum cystatin C(Cys‐C) ,β2 microglobulin(β2‐MG) and retinol binding protein (RBP) detection in different stages of hemorrhagic fever with renal syndrome(HFRS) .Methods The levels of serum Cys‐C ,β2‐MG and RBP were detected in 22 patients with HFRS and 30 cases of healthy physical examination and the detection results were statistically analyzed by the SPSS13 .0 software .Results The serum Cys C and β2‐MG levels in every stage of HFRS were increased compared with the healthy control group(P< 0 .05) ,which in the oliguria stage was most significant (P< 0 .01) .RBP had no significant differences in the fever stage and shock stage compare with the healthy control group (P >0 .05) ,while RBP was significantly increased in the oliguria stage ,polyuria stage and convalescence stage(P< 0 .01) ,but no statisti‐cally significant differences among the three stage(P> 0 .05) .Conclusion β2‐MG is more sensitive than Cys‐C in the early stage of HFRS ;RBP has the clinical guidance significance in the progression of HFRS .

Objective Discuss the interference of injection cefotiam on vanadate oxidation method and dry chemical method assay total bilirubin .Methods Collected 60 examples ,include total bilirubin concentration 20 examples less than 20 μmol/L ,20 examples between 150-220 μmol/L and 20 examples between 350-410 μmol/L ,add an equal volume of various concentrations of cefotiam in each case ,formulated into cefotiam final concentrations of 300 ,150 ,75 mg/L of serum samples as the test group ,add an equal volume of water in each serum samples as the control group ,determine all the samples total bilirubin concentration respectively by vanadate oxidation method and dry chemical method ,compared the interference of cefotiam on determined total bilirubin by two method ,analyze the data by SPSS13 .0 .Results Determined total bilirubin by dry chemical method ,the test group higher than the control group ,the difference was statistically significant(P<0 .05) ,at the same total bilirubin levels ,with cefotiam concentrations decreased ,increased rate of total bilirubin concentration were decreased in the experimental group .Determined total bilirubin by vanadate oxidation method ,when the total bilirubin concentration between 150 -220 μmol/L ,the test group was higher than the control group ,the difference was statistically significant(P<0 .05) .Conclusion Interference of injection cefotiam on determined to‐tal bilirubin by dry chemical method is strong ,and with the drug concentration increased ,effect is more obvious ,but determination of total bilirubin by vanadate oxidation method has almost no effect .

Objective To explore the moderating and mediating roles of psychological capital in the associations of effort?reward imbalance and overcommitment with job burnout among iron?steel workers. Methods Totally 1 700 male iron?steel workers from an iron and steel company were enrolled for the study. A set of questionnaires that included demographic and working factors,the Maslach Burnout Inventory?General Survey,the Ef?fort?reward Imbalance Scale and the Psychological Capital Questionnaire,as well as perceived work environment was distributed to the subjects. A to?tal of 1 254 effective respondents(73.76%)were collected. Hierarchical linear regression analysis was performed to explore moderating role,and as?ymptotic and resampling strategies were used to detect mediating role. Results There was no moderating role of psychological capital on the associa?tion between effort?reward imbalance and job burnout. Psychological capital had significant and positive moderating roles on the associations of over?commitment with emotional exhaustion and cynicism. Psychological capital showed significant mediating role in the associations of effort?reward im?balance with emotional exhaustion and cynicism,and the proportion of mediating role was 5.84%and 18.75%,respectively. Conclusion In work?ers with high level of psychological capital,emotional exhaustion and cynicism would be significantly increased with overcommitment,and there is a weak increasing trend of emotional exhaustion and cynicism in workers with low level of psychological capital. Effort?reward imbalance could lead to emotional exhaustion and cynicism through the reduction of psychological capital of iron?steel workers.

Mass cytometry is an emerging technology. Metal isotope tags are used to replace fluorescent tags, and are quantified by mass spectrometry. It combined mass spectrometry with flow cytometry, which could simultaneously measure various parameters without compensation and greatly improve the ability to evaluate complex cellular systems and processes. Its characteristics of high throughput, high sensitivity and high stability are especially suitable for the study in immunity, tumor, blood, medicine and genetics, etc.

Objective To evaluate the diagnostic accuracy of CMS50F for screening in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods Sixty-four volunteers with suspected OSAHS underwent simultaneous noc?turnal polysomnography (PSG), micromovement sensitive mattress sleep monitoring system(MSMSMS)and CMS50F. The ap?nea-hypopnea index (AHI) detected by PSG and MSMSMS was used as the diagnostic standard for OSAHS. The reliability of CMS50F for monitoring sleep was assessed. Results There was no statistic difference in CMS50F-ODI3 and PSG-AHI be?tween normal, mild and moderate OSAHS groups(P>0.05). The CMS50F-ODI3 was smaller than the PSG-AHI in severe OSAHS patients(P < 0.05). There was a positive correlation between CMS50F-ODI3 and PSG-AHI(r=0.855, P < 0.05). PSG-AHI≥5 events per hour was used as the threshold value to diagnose OSAHS, the sensitivity and specificity of CMS50F were 94.5%and 88.9%. There were no significant differences in CMS50F-ODI3 and MSMSMS-AHI between normal, mild and moderate OSAHS patients(P>0.05). The value of CMS50F-ODI3 was smaller than MSMSMS-AHI in severe OSAHS patients (P < 0.05). There was also a significant correlation between CMS50F- ODI3 and MSMSMS-AHI (r=0.867,P <0.05). MSMSMS-AHI≥5 events per hour was used as the threshold value to diagnose OSAHS, the sensitivity and specificity of CMS50F were 94.5%and 88.9%. Conclusion CMS50F can be used as a portable and reliable device for screening of pa?tients suspected OSAHS.

Subthalamic nucleus (STN) deep brain stimulation (DBS) has become an important surgical treatment of Parkinson disease, but its exact mechanism is still unclear.In this study, a 16-channel implantable microelectrode array (MEA) was prepared by micro-electromechanical system (MEMS) technique and later modified with platinum black/reduced Graphene Oxide/Nafion (Pt/RGO/Nafion) nanocomposites.Extracellular dopamine (DA) content and spike of dorsal striatum neurons were synchronously recorded before and after STN stimulation.The results showed that the dopamine content began to increase within 20 s after electrical stimulation and dropped to normal level after about 50 s, with the highest rising concentration of 1.72 μmol/L.At the same time, there was an increased spike activity of interneurons in the dopamine ascending phase, and the spike firing rate of medium spiny projection neurons (MSNs) was high when the concentration of DA was higher than the normal level.The MEA sensor can simultaneously record dopamine flux and physiological signals in situ, thus providing an ideal tool for neural information detection.

A lack of effective drugs and technical means to eradicate hepatitis B virus (HBV) is a bottleneck that limits the ability to fully cure HBV infection. Recently, genome-editing technology based on clustered regularly interspaced short palindromic repeats -associated protein 9 is an emerging technique for editing specific gene loci, which can specifically target HBV covalently closed circular DNA, effectively inhibits HBV DNA replication and regulates HBV functional protein expression, and is expected to become a powerful gene therapy tool for the complete eradication of HBV. Considering this, it has become the focus of attention for scholars at home and abroad that how to use clustered regularly interspaced short palindromic repeats -associated protein 9 to accomplish modification of HBV genomes for complete eradication of HBV. This paper summarizes the latest progress based on the latest research results at home and abroad in the application of clustered regularly interspaced short palindromic repeats -associated protein 9 gene editing technology in anti-HBV infection treatment, and expounds its potential and challenges as a radical cure for HBV infection.

By using R language to deal with practical problems,we introduce two methods of obtaining time related receiver operation characteristic [ROC(t)] curves from survival data:1) nearestneighbor estimator of bivariate distribution (NNE) estimation:to obtain cumulative/dynamic ROCC/D(t) curves;2) Cox estimation:to obtain incident/dynamic ROCI/D (t) curves.The areas under the ROC(t) curves (AUC) obtained from the two methods fluctuate over time.The one obtained through NNE has bigger fluctuation than that obtained through Cox,while the mean of AUC of the two methods are similar.Time related ROC (t) can be effectively used to evaluate the diagnostic capacity of the marker in clinical trials,and help to select the best diagnostic time of the marker.According to the different scientific interests,researchers should select relevant methods for more accurate evaluation.

Objective: To investigate the current status of occupational mental health and job satisfaction in university teachers in Shenyang, China and related influencing factors. Methods: A total of 1500 teachers from 6 universities in Shenyang were randomly selected as study subjects from November 2013 to January 2014. Self-administered questionnaires were used to investigate mental health, including effort-reward imbalance questionnaire, Minnesota Satisfaction Questionnaire, Center for Epidemiological Survey-Depression Scale, and Psychological Capital Questionnaire. Results: Of all teachers, 58.9% had depressive symptoms. Depressive symptoms in university teachers were negatively correlated with the scores of psychological capital, supervisory commitment, and perceived organizational support (r=-0.461, -0.306, and -0.366, all P<0.01) and were positively correlated with the score of occupational stress (r=0.414, P<0.01) . Job satisfaction was positively correlated with psychological capital, perceived organizational support, and supervisory commitment (r=0.650, 0.715, and 0.636, all P<0.01) and negatively correlated with occupational stress (r=-0.475, P<0.01) . The direct effects of occupational stress, perceived organizational support, and supervisory commitment on job satisfaction were -0.30, 0.26, and 0.14, respectively, and their indirect effects were -0.0176, 0.0656, and 0.0368, respectively. The direct effects of occupational stress, perceived organizational support, and supervisory commitment on depressive symptoms were 0.20, -0.08, and -0.05, respectively, and their indirect effects was 0.033, -0.123, and -0.069, respectively. Conclusion Occupational mental health is closely associated with job satisfaction in university teachers in Shenyang, and psychological capital has a mediating effect on perceived organizational support, supervisory commitment, occupational stress, job satisfaction, and depressive symptoms.