Objective To investigate the application of molecular absorbent recycling system (MARS) in auto liver transplantation (ALT).Methods The clinical data of 46 patients who underwent ALT in Zhongnan Hospital of Wuhan University from September 2014 to August 2015 were retrospectively studied.The patients were randomly divided into the MARS group (n =30) and the hemodialysis group (n =16).In the MARS group,20 patients were male and 10 were female with an average age of (15 ±67) years,and the median age was 46.In the hemodialysis group,8 were male and 8 were female with an average age of (22 ± 54) years.A heparinized left iliac vein was linked to a centrifugal pump of a MARS and an output tube was linked to a left jugular vein.Before the anhepatic phase,physical pretreatment was carried out by ligating the porta hepatis and inferior vena cava (IVC) to adapt the patient to the anhepatic phase.Subsequently,a bypass was established followed by ligation of the IVC.The duration of operation,length of stay in ICU after operation,one-year survival rate and hepatorenal function were studied.Results The duration of operation in the MRAS group and in the hemodialysis group was not significantly different (P ＞0.05).The length of stay in ICU after operation in the MARS group was (12.0 ±3.0) d,which was significantly shorter than that of the hemodialysis group (20.0 ± 2.0) d (P ＜ 0.05).The one-year survival rate in the MARS group was (100％),which was significantly higher than that of the hemodialysis group (93.8％,P ＜0.05).Conclusion The application of MARS in ALT shortened the length of stay in hospital and improved one-year survival rate,resulting in better outcomes than the conventional method.

Objective To prepare diosmin suppository and establish a method for its quality control.Methods Diosmin was used as active ingredient.Polyethylene glycol 6000, polyethylene glycol 400 and glycerol were chosen as substrates.Fusion method was applied to prepare the diosmin suppository.The appearance, content uniformity, weight variation and melting time of suppository were evaluated.Results The diosmin suppository has brown color with good hardness at room temperature.The content uniformity, weight variation, melting time and dissolution rate meet the requirements of quality control.Conclusion The diosmin suppository prepared by this method was satisfied the quality requirements of suppository and serves as a new formulation of diosmin.

Objective To explore relevant factors of drinking relapse after alcohol withdrawal for patients with alcohol dependence. Methods 130 patients with pure alcohol dependence who were admitted from January 2012 to February 2013 were selected. They received withdrawal treatment and were followed-up after discharge. They were assigned to relapse group and non-relapse group. The relations between drinking relapse and clinical characteristics, socio-demographic data, family environment and cognitive functions during withdrawal period in the two groups were analyzed. Results Excepted for 21 patients who were out of touch, there were 67 patients in the relapse group and 42 patients in the non-relapse group. Amount of drinking, frequency of drinking, personality changes, emotional symptoms, being single/divorced, finan-cial situation, education years, and family history of alcoholism before the treatment between the two groups were signifi-cantly different (P<0.05). Intimacy, emotional expression, independence, entertainment, organization, controlling and con-tradictoriness in the family environment between the two groups were significantly different (P<0.05). During withdrawal pe-riod, total response number, number of completed classification and correct response rate of attention (DST and CPT-IP), memory (S/VM, SS and DS) and execution (WCST) between the two groups were significantly different (P<0.05). Amount of drinking, emotional symptoms, family intimacy, family organization, family independence, attention and execu-tion were independent influencing factors of drinking relapse for patients with alcohol dependence after withdrawal (P<0.05). Conclusion Patients with alcohol dependence have a large capacity for alcohol previously, and patients with emo-tional symptoms, in lack of intimacy, organization and independence in family environment, or in lack of attention and ex-ecution during withdrawal period are highly likely to relapse. Management of family environment and psychological thera-py should be attached great importance so as to improve patients' cognitive function during withdrawal period.

Objective To investigate the changes of event-related potentials (ERPs) in patients with intelligence impairments after craniocerebral trauma.Methods 60 patients with intelligence impairments after craniocerebral trauma were enrolled as case group,and 60 healthy subjects were enrolled as control group.EEG instrument was used to record P300 and P50 of the two groups and the differences in P300 and P50 components were compared.Results There are significant differences between case group and control group in latency of P300 ((440.430 ± 77.367) ms vs (342.928 ± 36.175) ms,P＜ 0.01),and case group showed decreased amplitude ((12.692±8.152) μV vs (18.138±6.590) μV,P＜0.01).The S2-P50 amplitude of case group was significantly higher than that of control group ((3.75± 1.59) μV vs (2.42±1.43) μV,P＜0.01).In addition,the S2-P50 amplitude/S1-P50 amplitude ratio of case group was higher than that of the control group,and the difference was statistically significant (0.78±0.54 vs 0.46±0.18,P＜0.01).The latency and amplitude of P300 were significantly correlated with the total score of WAIS-RC (r=-0.31,P＜0.01;r=0.17,P＜0.01);The amplitude of S2-P50 and the ratio of S2-P50 amplitude to S1-P50 amplitude were significantly negatively correlated with the total score of WAIS-RC (r=-0.33,P＜0.01;r=-0.45,P＜0.01).Conclusion P300 and P50 components of ERP can provide references for judicial expertise to evaluate intelligence impairments after craniocerebral trauma.