Objective:To explore influencing factors of quality of life (QOL)in aged patients with coronary heart disease (CHD)and perform correlation analysis among quality of life and its influencing factors.Methods:A total of 70 aged CHD patients treated in our hospital from 2011 to 2015 were selected and followed up.All patients received evaluation of Chinese Questionnaire of Quality of life in Chinese patients with cardiovascular diseases (CQQC ). CQQC score was compared between patients with different gender,age,course of disease,body mass index (BMI) and number of complications.Spearman correlation analysis was used to analyze the correlation between influencing factors of QOL and CQQC score.Results:There was no significant difference in CQQC score between male and fe- male patients (P=0.697).CQQC scores of patients with age 60~70 years,course of disease <15 years,BMI<30 kg/m2 and no complications were significantly higher than those of patients with age 71~87 years,course of disease>15 years,BMI>30 kg/m2 and complications,P<0.01 all.Spearman correlation analysis indicated that age (r=-0.621),course of disease (r=-0.691)and BMI (r=-0.655)were significant inversely correlated with CQQC score,P<0.01 all.Fourfold table chi-square test found that for percentage of patients with CQQC score >50 scores,scores of 60~70 years were significantly higher than those of>70 years (85.42% vs.31.82%),scores of course of disease <15 years were significantly higher than those of course of disease >15 years (79.55% vs. 3.85%),scores of BMI<30 kg/m2 were significantly higher than those of BMI>30 kg/m2 (62.22% vs.20.00%) in aged CHD patients,P<0.01 all.Conclusion:Quality of life in CHD patients are mainly affected by age,course of disease and body mass index etc.So attention should be paid to follow-up,guiding therapy and intervening lifes- tyle in order to improve patients'quality of life.

Blood typing and compatibility testing directly affect curative effects of blood transfusion.To avoid severe blood transfusion reactions, more and more new immunohematology technologies and methods are becoming gradually applied to blood transfusion laboratories.Gel test, monocyte monolayer assay and analysis of blood group genes have been confirmed to be beneficial on improving the sensitivity and automatization.Some technologies can predict red cell survival in vivo more accurately.It can improve the efficacy of blood compatibility before transfusion by using these technologies.There is no general methods used in solving of complex serological problems, but medical staffs can provide appropriate blood for different sufferers by using different technologies at the right time.

The role of Sonic Hedgehog (SHH)signaling pathway in embryonic lung development has been recognized.What's more,it also plays a key role in postnatal development and maintenance of tissue or organ integrity and function.In recent years, studies have found that SHH signaling pathways are involved in the lung repairing process, suggesting that SHH signaling pathways may play a role in pulmonary fibrosis.This paper reviews the effect of the SHH signaling pathway in pulmonary fibrosis.

Objective To determine the expressions of transcription factor activator protein-2α (AP-2α), estrogen receptor-β (ER-β) and matrixmetalloproteinase-9 (MMP-9) in lichen planus (LP) lesions and their significance. Methods Tissue samples were resected from the lesions of 30 patients with LP and normal skin of 30 human controls. An immunohistochemical method using streptavidin-peroxidase (SP) was performed to detect the expression of ER-β and MMP-9, reverse transcription-PCR and Western blot to measure the mRNA and protein expressions of AP-2α, ER-β and MMP-9 in these specimens. Results There was a significant decrease in the mRNA expressions of AP-2α (0.488 ± 0.039 vs. 1.008 ± 0.023, P < 0.01 ) and ER-β (0.365 ± 0.032 vs. 0.998 ± 0.036, P < 0.01), together with an increase in the expression of MMP-9 (1.237 ± 0.027 vs. 0.567 ± 0.015, P< 0.01) in the LP lesions compared with the control specimens; similar results were observed for the protein expressions of AP-2α, ER-β and MMP-9. In LP lesions, the expression of AP-2α was positively correlated with that of ER-β (R = 0.89, P < 0.01), but negatively correlated with that of MMP-9 (r = -0.91, P < 0.01). Conclusions The down-regulation of ER-β expression and up-regulation of MMP-9 expression may be ascribed to the abnormal regulation of upstream target genes mediated by the decreased expression of AP-2α, which may be involved in the pathogenesis of LP.

Copy number variation (CNV) is one kind of structural variations,which exists extensively in human genome.In addition,it is the crucial source of inter-individual gene differences.Recently,with the successful development of gene array and gene sequencing,molecular methods for CNV testing are more and more standardizing and automating.Therefore,ongoing attentions have been attached to the effects and mechanisms of CNV in complicated diseases like autoimmune diseases.Molecular methods for CNV testing can be divided into two categories:target screening and the whole genome scanning platforms.Currently,the studies about CNV and autoimmune diseases mainly focused on systemic lupus erythematosus and rheumatoid arthritis.Considering the limitations of measurement methods and testing costs,it is still in the infant.

Objective · To understand current smoking status and analyze its influencing factors among university students in Shanghai, and provide reference and guidance for further efforts of tobacco control in campus. Methods · A total of 4816 students from 19 col eges in Shanghai were selected by stratified multi-stage cluster sampling and the sample size in each part was decided by proportion of col eges and types of specialities. Self-administered questionnaire was conducted to understand the current tobacco use and its influencing factors were analyzed. Results · The overal smoking prevalence of col ege students in Shanghai was 5.80%. General y, smoking prevalence of junior col ege students was higher than that of undergraduates (11.27% vs 3.68%, P<0.05) and smoking prevalence of male students was higher than that of females (11.10% vs 0.95%, P<0.05). Nonsmokers endorsed higher awareness on the harm of smoking and second-hand smoking than smokers(P<0.01). Besides, nonsmokers had a more positive attitude towards tobacco control policy than smokers (P<0.01). Students who studied in the junior college, males, in the senior grade, majored in liberal arts, with higher monthly living expenses, held negative attitude in raising cigarette prices and supporting of establishing smoke-free campuses were more likely to be smokers. Conclusion · Tobacco use among university students in Shanghai deserves attention. It is necessary to carry out systematic and in-depth education to prevent and reduce smoking among the university students.

Objective To discuss the influence of different concentration sulindac on pancreatic cancer cell line PANC-1 cell proliferation and apoptosis,and investigate the possible mechanism that sulindac can inhibit the Wnt/beta-catenin pathway to kill pancreatic cancer cells. Methods PANC-1 cell were divided into negative control group (added containing no sulindac DMSO)and experimental group (added sulindac with concentrations of 0.25 ,0.5 ,1 ,1.5 ,2 mM medium,respectively,name as 0.25 mM group,0.5 mM group,1.0 mM group,1.5 mM group,2.0 mM group),and treated with different time,cell proliferation inhibition ratio in each group was detected by MTT assay,cell apoptosis ratio was detected by flow cytometry,the expression ofβ-catenin mRNA and protein were detected by RT-PCR and immunocytochemistry.Results MTT results showed that sulindac can inhibit the cell proliferation of PANC-1 by a dose-and time-dependent manner.Cell apoptosis increased after sulindac treatment in different degrees,and there were statistical differences between 1.5,2.0 mMgroup and control groups (P<0.05).RT-PCR results showed that the expression ofβ-catenin mRNA decreased after the treatment of sulindac,there were statistical differences between 1.5,2.0 mMgroup and control group (P<0.05). In the 2.0mM group,the expression ofβ-catenin decreased along with the time extending (P<0.05 ).ICC results showed that sulindac inhibited the expression ofβ-catenin protein and nuclear accumulation,there were no statistical differences in 0.25 ,0.5 mM group and control group,but there were statistical differences in 1.0,1.5,2.0 mMgroup.Conclusion Sulindac could inhibit cell proliferation and facilitate apoptosis of PANC-1,this effect is dose-and time-dependent.The inhibition of Wnt/beta-catenin signal pathway may be a possible mechanism of its cytotoxicity.

Different maternal positions and movement are utilized gradually in clinic while today it appears that the majority of women in the world birth in supine position or lithotomy position. To know the current situation and progress of the studies concerning different maternal positions and movement during labour and to explore the current relevant problems in clinic. The search strategy was designed to identify the relevant literature. The search was limited to the last 20 years as current literature was sought. Thirty eight papers were identified as relevant and included in this literature review. The majority studies is concerning about the outcome of mother and fetus. There is no unitized clinical pathway currently, and the objects implemented are limited. There is no study related to the requirement of implementing different maternal positions and movement during labor in clinic and the effect of pelvic floor function after parturition. The positive effect of implementing different maternal positions and movement during labor can be sure for the process of parturition and the outcome of mother and baby. Childbirth in different maternal positions and movement is the trend in the future. Therefore, how to utilize different maternal positions and movement during labor well, it is still a huge challenge for medical staff. And more convincing studies and information are needed as evidence in clinic.

With the development of radiotherapy techniques,the efficacy of radiotherapy for liver tumors has been improved,and radiothera-py can be considered as an alternative treatment for patients who are not suitable for surgery.The progress in liver tumor radiotherapy repor-ted in the 55th annual meeting of the American Society for Therapeutic Radiology and Oncology (ASTRO)in 2013,particularly the efficacy and safety of stereotactic body radiation therapy for liver tumors,is reviewed.In the future,radiotherapy may be increasingly used as an ef-fective method in the treatment of liver tumors.The main studies reported at the meeting are sorted as follows in this article.

In the present study, the regulation of Vitreoscilla hemoglobin (VHb) on astragaloside IV biosynthesis was investigated. An intermediate expression vector consisting of the CaMV35S promoter fused to the vgb and nopaline synthase terminator was transferred into Astragalus membranaceus via Agrobacterium rhizogenes. The transgenic hairy roots were confirmed by PCR amplification and Southern blot hybridization. The expression of vgb in transgenic hairy roots was confirmed by RT-PCR. After 15 days cultivation, the dry weight and growth rate of transgenic hairy roots were higher than that of the non-transgenic hairy root. ELSD-HPLC analysis showed that astragaloside IV content of transgenic hairy roots was 5 to 6 times of non-transgenic hairy root control and 10 to 12 times of Radix Astragali from Shanxi Province. These results suggested that the expression of vgb promoted the growth of transgenic hairy roots, and increased the content of astragaloside IV.