BACKGROUND:Superparamagnetic iron oxide (SPIO) labeling can trace the migration of stem cells in vivo, and the fluorescent DiI dye is suitable for marking and tracing cells because of its less influence on cellviability, proliferation and differentiation.
OBJECTIVE:To observe the effect and safety of SPIO and fluorescent DiI dye to double label bone marrow mesenchymal stem cells.
METHODS:The bilateral lower limbs of rats were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. Bone marrow mesenchymal stem cells were isolated by the whole bone marrow adherence method and purified by differential attachment method. Purified cells were dual-labeled with SPIO particle and fluorescent DiI dye.
RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells could be separated at 8-10 days after primary culture and the subculturing cycle was 3-4 days. Rat bone marrow mesenchymal stem cells could be effectively labeled with SPIO-DiI and the labeling efficiency was almost 100%. Blue irons contained in intracytoplasmatic vesicles could be observed clearly with Prussian blue staining, and the fluorescence microscopy showed red fluorescence at cytoplasm. Survival and apoptosis percentages obtained by MTT analysis were similar among labeled and unlabeled bone marrow mesenchymal stem cells that were both about 95%.These findings indicate that the rat bone marrow mesenchymal stem cells could be efficiently labeled with SPIO-DiI to construct a nano-bioprobe, without significant changes in morphology, viability and proliferation.

Objective To analyze research literature about integration of the body and the spirit; To understand the research status of this classical theory.Methods Relevant articles about integration of the body and the spirit in CNKI, Wanfang Data, VIP-Chinese Scientific and Technological Journal Data were searched, and bibliometric analysis with NoteExpress3.0, Bicomb2.0 and SPSS19.0 software was used to analyzed the data.ResultsA total of 156 efficient articles were retrieved. The number of published articles increased, but the core author group had not yet been formed. The number of cited articles was low. The study hot spots mainly focused on TCM constitution, the subject of health preservation, TCM basic theories, and psychosomatic medicine.Conclusion Domestic studies on integration of the body and the spirit are still in the initial stage. Research number should be increased, meanwhile, innovation and quality should be emphasized.

Objective To investigate the clinical features of polymyositis/dermatomyositis (PM/DM) with anti-nuclear matrix protein (anti-2NXP2) antibodies in Han-Chinese.Methods ImmunoprecipitationWestern Blotting (IP-WB) method was used for screening anti-NXP2 antibodies in 141 adult Han-Chinese patients with PM/DM.The clinical and laboratory data were collected,analyzed and compared with the antiNXP2-positive patients reported in the literature.Statistical analyses were performed using chi-square test and Fisher's exact test.Results Seven (5％) patients,including 6 with DM and 1 with PM,were identified as anti-NXP2 positive.Consistent with the 114 anti-NXP2-positive patients reported in the literature,Han patients with anti-NXP2 had higher frequencies of weakness,heliotrope rash and Gottron's sign.Compared with anti-NXP2-negative patients,anti-NXP2-positive patients presented significantly higher frequencies of dysphagia [43％(3/7) vs 9％(12/134),x2=8.04,P=0.027] and edema [43％(3/7) vs 2％(3/134),x2=26.94,P=0.001 4],while an absence of PM/DM related cancer was observed in Han patients with anti-NXP2.Conclusion Adult Han-Chinese patients with anti-NXP2 are DM predominant,and are characterized by a high frequencies of edema and dysphagia.

Worster-Drought syndrome (WDS) is a special and rare type of cerebral palsy, which is characte-rized by pseudobulbar palsy, selective weakness of the muscles of the lips, tongue, and soft palate, dysarthria, dysphagia, salivation, and jaw spasm.The onset of WDS is linked with a variety of prenatal, intrapartum and postnatal factors.The report of family cases of WDS suggested the genetic predisposition.Although there is no cure at present, early genetic counseling, early diagnosis and intervention can reduce the morbidity of offspring and improve the symptoms of children with WDS, which will greatly reduce the burden on the family and society in the future.In the present study, this paper aims to explore the etiology, pathogenesis, clinical manifestation, imaging examination, diagnosis, disease spectrum and current treatment of WDS, thus improving the diagnostic and therapeutic efficacy.

Post-stroke depression (PSD) is an important mental complication of stroke, affecting nearly 1/3 of stroke patients, seriously affecting patients' functional recovery and quality of life, and is associated with increased mortality of stroke patients. Traditional antidepressant treatments include medication and psychotherapy, but there may be problems with adverse reactions, tolerance, or limited effectiveness. Repetitive transcranial magnetic stimulation (rTMS), as a non-invasive neuroregulatory technique, offers a new treatment option for patients with PSD. This article reviews the application of rTMS in the treatment of PSD and its possible mechanism.

Objective To investigate the value of low-dose multi-slice CT (MSCT) chest scan in the diagnosis of coal workers' pneumoconiosis.Methods A total of 90 patients with a confirmed diagnosis of coal workers' pneumoconiosis were enrolled,and under the conditions of fixed tube voltage,pitch,and slice thickness,they underwent CT scan with a normal dose (150 mA) and a low dose (30-50 mA).The quality of images obtained from two scans was compared,and the imaging findings,opacity profusion,stage,and radiation doses were also compared.Results Compared with the normal-dose scan,low-dose scan increased the image noise,and the images obtained from scans with doses of 30,40,and 50 mA did not show significant reductions in signal-to-noise ratio or contrast-to-noise ratio (P＞0.05).There was no significant difference in the percentage of image quality between low-dose and normal-dose scans (P＞0.05).There were no significant differences in the percentage of various imaging findings,opacity profusion,or percentage of different stages between low-dose (30,40,and 50 mA) and normal-dose (150 mA) scans (P＞0.05).Conclusion There are no significant differences between low-dose MSCT chest scan and normal-dose CT in image quality,imaging findings of coal workers" pneumoconiosis,opacity profusion,and stage.Meanwhile,low-dose MSCT chest scan greatly reduces the radiation dose and can be used to assist the diagnosis and follow-up reexamination of coal workers" pneumoconiosis and cover the shortage of high-kilovoltage chest X-ray.

Objective To investigate the value of low-dose multi-slice CT (MSCT) chest scan in the diagnosis of coal workers' pneumoconiosis.Methods A total of 90 patients with a confirmed diagnosis of coal workers' pneumoconiosis were enrolled,and under the conditions of fixed tube voltage,pitch,and slice thickness,they underwent CT scan with a normal dose (150 mA) and a low dose (30-50 mA).The quality of images obtained from two scans was compared,and the imaging findings,opacity profusion,stage,and radiation doses were also compared.Results Compared with the normal-dose scan,low-dose scan increased the image noise,and the images obtained from scans with doses of 30,40,and 50 mA did not show significant reductions in signal-to-noise ratio or contrast-to-noise ratio (P＞0.05).There was no significant difference in the percentage of image quality between low-dose and normal-dose scans (P＞0.05).There were no significant differences in the percentage of various imaging findings,opacity profusion,or percentage of different stages between low-dose (30,40,and 50 mA) and normal-dose (150 mA) scans (P＞0.05).Conclusion There are no significant differences between low-dose MSCT chest scan and normal-dose CT in image quality,imaging findings of coal workers" pneumoconiosis,opacity profusion,and stage.Meanwhile,low-dose MSCT chest scan greatly reduces the radiation dose and can be used to assist the diagnosis and follow-up reexamination of coal workers" pneumoconiosis and cover the shortage of high-kilovoltage chest X-ray.

Artemisinin is an anti-malarial drug with poor water solubility and oral absorption; so a variety of derivatives based on the parent nucleus have been developed.Compared with artemisinin , dihydroartemisinin (DHA) has a stronger anti-malaria activity, and has the advantages of high metabolic rate and better water solubility .Recent studies have discovered that DHA has a good inhibitory effect on tumor cells, which is closely related to the peroxide bridge in its molecular structure . Since tumor cells need more Fe 3+ than normal cells, there are a large number of transferrin receptors on the tumor cell membrane .DHA can break the peroxide bridge in the presence of Fe 2+, and the free radicals generated can play its lethal effect on tumor cells.In addition, DHA can promote endocytosis of transferrin receptor , and thus prevent cancer cells from taking Fe 3+from microenvironment .This article reviews the anti-tumor molecular mechanism of DHA , including accelerating oxidative damage , inducing apoptosis , inhibiting the growth , proliferation and invasion of tumor cells , reversing tumor multidrug resistance .

Objective A lentivirus-mediated colon cancer cell line stably overexpressing human SAMHD1 gene was constructed to observe the effect of this gene on the ability of the cells to resist herpes simplex virus type 1 (HSV-1) infection. Methods p CDH-EF1-MCS-SAMHD1-T2 A-GFP recombinant plasmid was constructed using pCDH-EF1-MCS-T2 A-copGFP lentiviral vector. After confirming successful synthesis with sequencing, the recombinant plasmid and lentivirus packaging plasmids were co-transfected into 293 T cells. The pseudovirus solution was collected and concentrated, then human colon cancer cells were infected with the concentrated solution. The cell line overexpressing SAMHD1 gene was infected with HSV-1 in contrast to the control group, and the virus infection efficiency was assessed by Western blotting and immunofluorescence analyses. Results The pCDH-EF1-MCS-SAMHD1-T2 A-GFP recombinant plasmid was successfully constructed and verified by gene sequencing. Western blotting confirmed the overexpression of the SAMHD1 gene with higher levels of the gene in the transfected cells in contrast to control human colon cancer cell line. Western blotting and immunofluorescence showed that the cell line overexpressing human SAMHD1 gene could effectively inhibit the infection of HSV-1. Conclusion Lentivirus-mediated stable cell line overexpressing human SAMHD1 gene was effective in inhibiting HSV-1 replication and could help us to further investigate the function of SAMHD1.

Objective To investigate the effects of salvianolic acids(SA) on improving cerebral ischemic damage through silencing information regulator protein 1 (SIRT1)/high-mobility group box 1 (HMGB1) signal pathway in rat. Methods 132 male general-grade healthy SD rats were randomly divided into four groups:sham operation group(Sham),ischemia model group(IS),SA group and inhibitor group(EX527). Focal cerebral ischemia was induced by occlusion of right MCA for 2 h. The neurological deficits were assessed by modified Neurological Severity Scores (mNSS) at 1,3,and 7 days after reperfusion. The concentrations of SIRT1,HMGB1 mRNA in peri-ischemic brain tissues were detected by RT-PCR at 1,3 and 7 days after reperfusion. SIRT1,HMGB1,P-53 and NF-κB protein expression were detected by Western blot. Serum P-53,NF-κB levels were measured by ELISA. The histomorphology in peri-ischemic brain tissues was observed by HE staining first day after reperfusion. The terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) method was used to detect the neuronal apoptosis index. Results (1)Compared with IS and EX527 group,the mNSS score in SA group was significantly lower at 1 d,3 d and 7 d after reperfusion. (2)The expressions of HMGB1,P-53 and NF-κB in SA group were significantly lower than those in IS and EX527 group 7 d after reperfusion. The expression of HMGB1,P-53 and NF-κB protein in EX527 group was lower than that in IS group while it was significantly higher than the sham group 7 d after reperfusion. The expression of SIRT1 protein in SA group was significantly higher than that in IS and EX527 group,while the expression of SIRT1 protein in EX527 group was significantly higher than the sham group and IS group. (3)The expression of HMGB1 mRNA in SA group was significantly lower than that in sham group,IS group and EX527 group in the 7 d. The expression of HMGB1 mRNA in EX527 group was significantly higher than that in IS group and lower than that in sham group. The expression of SIRT1 mRNA in SA group was significantly higher than that in IS group,EX527 group and sham group. The expression of SIRT1 mRNA in EX527 group was significantly higher than that in sham operation and IS group. (4)The expressions of P-53 and NF-κB in peripheral blood of rats in SA group were significantly lower than those in IS group and EX527 group while the expression of P-53 and NF-κB protein in EX527 group was significantly lower than that in IS group and was higher than that in sham group at different time points. (5)The neuronal apoptosis index in SA group was significantly lower than that of IS group and EX527 group (P<0.05),while the neuronal apoptosis index of EX527group was significantly lower than that of IS group (P<0.05). Conclusion SA alleviate cerebral ischemc injury in rat by promoting SIRT1 transcription,inhibiting HMGB1 migration and expression,reducing the release of inflammatory factors P-53 and NF-κB in downstream pathways,and inhibiting neuronal apoptosis.