1.Isolation and Identification of two Escherichia albertii strains in Shanxi Province, China
Jitao WANG ; Dequan MENG ; Jiane GUO ; Guodong YAN ; Ling ZHANG ; Ziting WAN ; Qun LI ; Li GAO ; Ruihong GAO ; Lifeng ZHAO ; Hong WANG
Chinese Journal of Preventive Medicine 2020;54(10):1121-1126
Objective:To investigate the prevalence of Escherchia albertii in Shanxi province. Methods:The chicken intestines were enriched in EC broth. The eae gene was detected by PCR, and the eae-positive EC enrichments were inoculated in MacConkey agar plate. The eae-positive lactose non-fermenting isolates were presumed as Escherchia albertii, and then analyzed by triplex-PCR, 16S rDNA sequencing and MLST. Results:Two suspected Escherchia albertiiwere isolated from 250 samples of chicken intestines. It was identified as Escherchia albertii by phenotypic, specific genes,16S rDNA sequencing, and MLST analyses . The cytolethal distending toxin B ( cdtB) showed positive by PCR,and they were clusted to Ⅱ/Ⅲ/Ⅴ group by sequencing. Conclusion:This study showed that the Escherchia albertii was existed in Shanxi province, China.
2.Isolation and Identification of two Escherichia albertii strains in Shanxi Province, China
Jitao WANG ; Dequan MENG ; Jiane GUO ; Guodong YAN ; Ling ZHANG ; Ziting WAN ; Qun LI ; Li GAO ; Ruihong GAO ; Lifeng ZHAO ; Hong WANG
Chinese Journal of Preventive Medicine 2020;54(10):1121-1126
Objective:To investigate the prevalence of Escherchia albertii in Shanxi province. Methods:The chicken intestines were enriched in EC broth. The eae gene was detected by PCR, and the eae-positive EC enrichments were inoculated in MacConkey agar plate. The eae-positive lactose non-fermenting isolates were presumed as Escherchia albertii, and then analyzed by triplex-PCR, 16S rDNA sequencing and MLST. Results:Two suspected Escherchia albertiiwere isolated from 250 samples of chicken intestines. It was identified as Escherchia albertii by phenotypic, specific genes,16S rDNA sequencing, and MLST analyses . The cytolethal distending toxin B ( cdtB) showed positive by PCR,and they were clusted to Ⅱ/Ⅲ/Ⅴ group by sequencing. Conclusion:This study showed that the Escherchia albertii was existed in Shanxi province, China.