China Pharmaceutical University proposed and established the first second-level discipline of pharmaceutical pedagogy in China under the first-level discipline of pharmacy in 2021.Through exploring the value of pharmaceutical pedagogy and clarifying its connotation and development path, the construction of the discipline will be firmly pushed forward with a good start, laying a solid foundation for the connotative and high-quality development of pharmaceutical education in China.Using comparison, literature review and historical survey, iconic discipline factors in similar disciplines of pharmaceutical pedagogy, like medical pedagogy, nursing pedagogy, health education and social & administrative pharmacy were investigated.Our researches showed that pharmaceutical pedagogy in its narrow sense refers to the discipline of studying the phenomena and problems of pharmaceutical education with the cultivation of pharmaceutical talents as the core, and revealing the laws and characteristics of pharmaceutical education.The construction of pharmaceutical pedagogy should be further promoted with more academic achievements, stronger academic teams, organizations and journals with better and higher platforms of academic research.It was suggested that pharmaceutical pedagogy should be guided by Marxist philosophy in improving its academic system, academic institutions, academic teams and ability in participating in academic governance independently and openly.Pharmaceutical pedagogy is expected to accelerate the construction of theoretical system and discourse system of pharmaceutical education with Chinese characteristics, aiming to provide stronger intellectual support and knowledge contribution for pharmaceutical education in China and for China.

This study aims to investigate the function of lmo2363 gene in stress resistance of Liste-ria monocytogenes strain LM83-1.In this study,the lmo2363 gene deletion strain and complement-ation strain of Listeria monocytogenes were constructed using overlapping extended PCR and ho-mologous recombination techniques,and the growth ability,stress survival rate and biofilm forma-tion ability of wild,deletion strain and complementation strain were compared under different stress environments.lmo2363 gene deletion strain and complementation strain of Listeria monocy-togenes were successfully constructed in this experiment.The growth curves showed that the growth capacity of the deletion strain was weaker than the wild strain LM83-1 under 4 ℃,7％NaCl,10％NaCl,3.5％ethanol,4.0％ethanol and pH5 stress(P＜0.001).The results of stress survival test showed that the survival rate of the deletion strain was significantly lower than the wild strain after 1 h treatment with pH3 and 10 mmol/L H2 O2 stress(P＜0.010).The biofilm forming ability of the deletion strain was decreased compared with that of the wild strain(P＜0.050).This study confirmed that lmo2363 gene mediated the adaptation of LM to low temperature,high osmotic pressure,ethanol and acid stress environment and affected the formation of LM bio-film.This study laid a foundation for further exploring the function of lmo2363 gene in the stress resistance process of Listeria monocytogenes.