Objective:To explore the relationship between work connectivity behavior after-hours, self-regulatory fatigue and work engagement of nurses, so as to provide reference for developing strategies to improve nurses′work engagement.Methods:This was a cross-sectional survey study. A total of 661 nurses from 10 hospitals in Shandong Province were selected as subjects by convenience sampling method from June to August 2022. The Work Connectivity Behavior After-hours Scale, Self-Regulatory Fatigue Scale and Work Engagement Scale were used to measure the work connectivity behavior after-hours, self-regulatory fatigue level and work engagement level. A structural equation model was constructed to evaluate the mediating effect of self-regulatory fatigue on work connectivity behavior after-hours and work engagement.Results:The scores of work connectivity behavior after-hours, self-regulatory fatigue and work engagement of nurses were (37.66 ± 7.05), (42.98 ± 10.55) and (34.29 ± 6.58) points, respectively. The total effect of work connectivity behavior after hours on work engagement was -0.336 6. The work connectivity behavior after-hours was positively correlated with self-regulatory fatigue ( r = 0.423, P<0.01) and work engagement was negatively correlated with work connectivity behavior after-hours and self-regulatory fatigue ( r = -0.361, -0.479, both P<0.01). Self-regulatory fatigue had a partial mediating effect on work connectivity behavior after-hours and work engagement, accounting for 46.61% of the total effect. Conclusions:Self-regulatory fatigue has a mediating effect on the relationship between nurses′ work connectivity behavior after-hours and work engagement. Nursing managers should reduce the degree of self-regulatory fatigue of nurses by preventing their work connectivity behavior after-hours, so as to improve the work involvement level of nurses.

OBJECTIVE: To investigate the effect of n-hexane on the level of sex hormones and expression of estrogen receptor(ER) in rats and the protective effect of Lyciumbarbarum polysaccharide(LBP) on n-hexane-induced reproductive toxicity. METHODS: Based on factorial design model of 4×2, specific pathogen free adult female SD rats were divided into control group and low-, medium-and high-n-hexane exposure groups, and each group was divided into non-LBP intervention and LBP intervention sub-group. There were 8 subgroups with 6 rats in each group. On the first day, the rats in the 4 groups were given intraperitoneal injection of n-hexane at 0, 675, 1 350 and 2 700 mg/kg body weight, respectively. On day 2-4, the rats in the non-LBP intervention subgroup were given intragastric administration of 0.9% sodium chloride solution, and the rats in the LBP intervention subgroup were given intragastric administration of LBP at 50 mg/kg body weight once a day. On the fifth day, all animals were sacrificed, and the levels of follicle stimulating hormone(FSH), luteinizing hormone(LH), estradiol, progesterone were detected by enzyme linked immunosorbent assay. The mRNA expression of Erα, Erβ and G protein coupled estrogen receptor 1(Gper1) was detected by real time fluorescence polymerase chain reaction, and the expression of ERα, ERβ and GPER1 protein was detected by Western blotting. RESULTS: i) In the absence of LBP intervention(i.e. simple n-hexane exposure), there was no significant difference in the level of serum FSH, LH, estradiol and progesterone in the 4 groups(P>0.05). The relative expression of Erβ mRNA in ovary of low dose group decreased, while the relative expression of proteins of ERα and GPER1 increased(P<0.05) when compared with the control group. The relative expression of Erα mRNA and GPER1 protein in the ovary of medium-and high-dose groups increased(P<0.05), while the relative expression of Erβ, Gper1 mRNA and ERβ protein decreased(P<0.05). The relative expression of ERα protein in ovary of high-dose group increased(P<0.05). ii) At the same dose of n-hexane exposure, the relative expression of Erα mRNA in ovary of rats in low dose group increased(P<0.05), while the relative expression of ERβ and GPER1 protein decreased in LBP intervention group compared with the no LBP intervention group(P<0.05). The relative expression of ERα and GPER1 protein in ovary of medium dose group increased(P<0.05), while the relative expression of Gper1 mRNA and GPER1 protein in ovary of high dose group decreased in LBP intervention group compared with the no LBP intervention group(P<0.05). CONCLUSION: n-Hexane can up-regulate the expression of ERα and GPER1 in rat ovary, but has no significant effect on female endocrine system. LBP may play a protective role in female reproductive system by up-regulating the expression of ERα and GPER1.