Objective To study the effects of Xinkeshu capsule (mainly com po sed of Radix Salviae Miltiorrhizae, Radix Puerariae, Fructus Crataegi, Radix Not oginseng, Radix Auklandiae,etc.) on hemorheology of experimental atherosclerosis rabbits. Methods FASCO- 300 viscosity meter was used to detect whole blood v iscosity and plasma viscosity, hematocrit (Wen's tube) and erythrocyte sedimen tation (Wintrobe tube) were tested, and other items of hemorheology were exami ned. Results Xinkeshu capsule significantly inhibited the abnormal rise of who le blood viscosity, plasma viscosity, whole blood reduced viscosity, erythr ocyte aggregation index, erythrocyte sedimentation (ES), ES rate K, erythro cyte rigid index and Tk in the rabbits with atherosclerosis induced by high fat foodd( P

Objective To observe the clinical efficacy of acupuncture plus medication in treating sudden hearing loss.Method Sixty patients with sudden hearing loss were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by acupuncture plus medication, while the control group was intervened by medication alone. The two groups were treated once a day, 10 d as a treatment course. The pure-tone hearing threshold, plasma endothelin (ET) content, comparative and absolute counting of endothelial progenitor cells (EPCs) were observed before the treatment and after 2 treatment courses, and the clinical efficacies were compared. Result The pure-tone hearing threshold, plasma ET content, comparative counting and absolute counting of EPCs were significantly changed after the treatment in both groups (P<0.05). After the intervention, the pure-tone hearing threshold, plasma ET content, comparative counting and absolute counting of EPCs in serum of the treatment group were significantly different from those of the control group (P<0.05). The total effective rate was 92.6% in the treatment group versus 82.1% in the control group, and the between-group difference was statistically significant (P<0.05).Conclusion Acupuncture plus medication is an effective method in treating sudden hearing loss.

Purpose:To detect DNA polymerase ? gene (pol?) mutations in human gastric cancer specimens and the relationship between H.pylori infection and pol? mutation. Methods:Extracting total RNA from gastric carcinoma,corresponding cancer specimens tissues and normal tissues, synthesizing cDNA and then using them as templates proceed PCR,The products of PCR were checked by SSCP. Extracting DNA from the specimen, we could detect the H.Pylori from the tissue of gastric carcinoma and the tissue adjacent to them by PCR.Results:There were 7 abnormal SSCP of 32 gastric carcinoma samples, and the mutation rate was 21.9%, but nothing abnormal was found in the tissues adjacent to the tumor. The results of H.Pylori DNA were positive in 15 samples from 32 gastric carcinoma tissues. Positive rate was 46.9(15/32).Detection result of tissues adjacent to tumor was consistent with gastric carcinoma. Comparing pol? SSCP to H.pylori-DNA in gastric carcinoma,we found the positive samples of pol? SSCP correlated with H.pylori-DNA.Conclusions:It is suggested that the pol?gene mutation may be associated with carcinogenesis and development of human gastric cancer. H.pylori infection is possibly related to pol? mutation in gastric carcinoma.

Objective To study the expression and clinical significance of toll-like receptor (TLR) 4 and TLR7 in primary nephrotic syndrome (PNS) of different pathological types in children. Methods Renal expressions of TLR4 and TLR7 were amined and analyzed retrospectively in renal biopsy specimens from 110 PNS patients and 21 healthy controls by immunohisto-chemical method. According to the renal pathologic classification of PNS, the TLR4 and TLR7 expression levels in PNS of dif-ferent types were compared. Results Compared with the renal tissue of healthy controls, the expression level of TLR4 on renal tissue of PNS patients was significantly increased (P<0.01). Among MN, MsPGN and FSGS, the highest expression of TLR4 was observed in MCD (P<0.01). Compared with the renal tissue of healthy controls, the expression level of TLR7 in re-nal tissue of PNS patients was also significantly increased (P<0.01). Among MCD, MN and FSGS, the highest expression of TLR7 was observed in MsPGN (P<0.01). Conclusions TLR4 and TLR7 expression levels are increased in renal tissue of PNS patients and the expression levels may be correlated with renal pathological types.

The learning theory of constructivism, formed by the development of cognitive psychology, shaped well thecognitive structure of medical students. It paid attention to the curriculum configuration and integration, and also thoughtmuch of the situated instruction. The theory embodied the main ideology of regarding medical students as the instruc-tional central and the principle of independent study. It offered the theoretical basis and a new view for the setting ofclinical integrate curriculum.

Objective To observe the clinical efficacy of acupuncture in treating subjective tinnitus.Method Seventy subjective tinnitus patients were randomized into a treatment group and a control group, 35 cases in each group. The control group was intervened by conventional medication, while the treatment group was by acupuncture in addition to the medication given to the control. The serum zinc level, copper level, and pure-tone hearing threshold were observed before and after intervention, and the clinical efficacies were compared.Result After intervention, the serum zinc and copper levels, and pure-tone hearing threshold were significantly changed in both groups (P<0.05). The serum zinc and copper levels, and pure-tone hearing threshold of the treatment group were significantly different from that of the control group after intervention (P<0.05). The total effective rate was 93.8% in the treatment group versus 87.9% in the control group, and there was a significant difference between the two groups (P<0.05).Conclusion Acupuncture plus medication is an effective way in treating subjective tinnitus.

Objective To observe the effect of RNAi that silences MTA1 gene on invasion and migration of esophageal carcinoma 9706 cells. Methods The siRNA expression vector that silences MTA1 gene was transfected into EC9706 cells by liposome. MTA1 mRNA and protein expressions were detected through quantitative RT-PCR and Western blot, respectively. The invasion and migration of EC9706 cells were evaluated by scrape wound healing assay and cell invasion assay in vitro. Results MTA1 gene expression significantly decreased. The scrape wound of EC9706 cells healed more slowly and the cell population that cut through Matrigel were less in the EC9706 cells transfected with siRNA expression vector than non-transfected EC9706 cells and the EC9706 cells transfected with blank vector (P

Objectives To observe the expressions ofα-smooth muscle actin (a-SMA) and type III collagen (Col-III) of tubuloin-terstitial ifbrosis(TIF) induced by unilateral ureteral obstruction (UUO) in rat and the intervention effect of supplemental hydrogen sul-ifde (H2S). Methods Ninety-six male Sprague-Dawley rats were randomly divided into 4 groups, sham-operated group, UUO model group, NaHS low-dose group and high-dose group. TIF rat model was established via UUO. After UUO operation, low-dose and high-dose group were intraperitoneally injected twice a day with 1.4μmol/kg and 7.0μmol/kg NaHS, respectively. Sham-operated group and UUO model group were given an equivalent volume of normal saline. Eight rats in each group were killed randomly at 7, 14 and 21 days after UUO operation. The concentration of plasma H2S was detected using deproteinization. Renal tubulointerstitial damage was evaluated with routine Hematoxylin and Eosin staining and Masson staining under microscope. The expressions ofα-SMA, Col-III were measured with immunohistochemistry. Results Compared with sham-operated group, renal tubulointerstitial injury was severer in UUO model group and was alleviated after intervention of NaHS. There was signiifcant difference in tubulointerstitial injury among all groups (P<0.01), but no difference was found between high-dose and low-dose group. Exogenous H2S supplement could down-regu-late the expressions ofα-SMA and Col-III in renal tissues (P<0.05). There was no difference between high-dose and low-dose group in the expressions ofα-SMA and Col-III (P>0.05). Conclusions TIF induced by UUO is associated with decreased level of endogenous H2S. H2S supplementation can ameliorate the development of UUO-associated TIF in part through down-regulating the expressions ofα-SMA and Col-III in renal tissues. However, a dose dependent manner between the two doses of exogenous H2S supplementation was not observed.

Objective To understand the overall development of TCM technique patent in Guangdong Province through the analysis on the patent application and authorization status of TCM technique patent in Guangdong Province from 2006 to 2015. Methods CNIPR and State Intellectual Property Office platform was searched, and 2006-2015 TCM technique patent application literature in Guangdong Province were obtained, the patent application and authorization status were analyzed. Results The number of application for TCM technique patent in Guangdong Province was 1434 during 2006-2015, and the number of application and authorization rates were increasing. The number of application in the Pearl River Delta (PRD) region of Guangdong Province was greater than that of the non-PRD regions, with individual application of 65.9% and enterprise application of 25.9%; A61K36 accounted for 98.7% in TCM technique patent classification statistics. Conclusion The quantity of patent application of TCM technique in Guangdong Province is large, but the number of application of non-individuals, the rate of authorization, technology statistical classification and innovation ability of TCM technique are low.

Objective To compare the therapeutic effect of Qingre Anchuang Tablets(QAT) processed by new or old technology on rabbit ear acne.Methods Experimental rabbit ear acne was induced by coal tar.After the modeling,the rabbits were administrated with QAT processed by new or old technology.The thickness,weight and PGE2 level of the acne ear were measured,and the pathological changes of the acne ear were also examined.Results QAT processed by new or old technology could decrease the thickness,weight and PGE2 level of the ear significantly,and relieve the pathological changes of the acne ear.The effects of QAT processed by new technology was better than that of QAT processed by old technology.Conclusion QAT processed by new technology has a better therapeutic effect on rabbit ear acne than QAT processed by old technology.