Objective To study the effects of Alternariol(AOH) on DNA polymerase ?(DNA POL?)expression in NIH3T3 cells.Methods RT-PCR,Immunocytochemistry and Western blot were used to detected mRNA and the protein levels of DNA POL? in NIH3T3 cell line induced by AOH.Results The expression of DNA POL? in NIH3T3 cells contaminated by AOH was significantly higher than that in the control group(P

Objective:To explore the application value of the suction-flushing electric coagulation in surgery of the traumatic splenic rupture.Methods:The clinical data of 31 patients with traumatic splenic rupture in the Affiliated Huai′an No.1 People′s Hospital of Nanjing Medical University from January 2013 to December 2019 were retrospectively analyzed. All patients were treated with suction-flushing electric coagulation.Results:The intraoperative hemorrhage was 100 to 1 200 (551 ± 90) ml, the operative time was 60 to 210 (113 ± 36) min, and the postoperative fluid drainage volume was 120 to 650 (367 ± 66) ml. All patients recovered successfully after surgery, electrocoagulation hemostasis was performed in 5 patients with splenic rupture, 25 patients with total splenectomy, and spleen preserving surgery in 1 patient with spleen preserving surgery after pancreatic body transection. There were no splenic infarction, splenic infection and portal vein thrombosis after 1 to 6 months′ follow-up.Conclusions:Suction-flushing electric coagulation is convenient and effective in the treatment of traumatic splenic rupture.

The retinal organoid is one of the most widely studied organoids in vitro, which is an "organ in a dish" with a highly similar tissue structure to those in vivo.Since it was first reported, the differentiation methods of retinal organoids have been continously developed to improve the differentiation efficiency and achieve further maturation.Retinal organoids have a broad application prospect as developmental and disease models, as well as seed cells for replacement therapy.However, there are still some problems to be solved, including the universality, effectiveness, heterogeneity of retinal organoid differentiation, as well as the difference between retinal organoids development and the real fetal retinal development.Researchers should investigate the regulational mechanism during organoid development from molecular, genetic, cellular and histological structure levels in order to promote the clinical practices of organoid culture technology.

The morphology of the alveolar bone at the maxillary anterior teeth in periodontitis patients was evaluated by cone-beam computed tomography (CBCT) to investigate the distribution of alveolar defects and provide guidance for clinical practice. Ninety periodontitis patients and 30 periodontally healthy individuals were selected to determine the morphology of the alveolar bone at the maxillary anterior teeth according to the degree of bone loss, tooth type, sex and age. The differences in the dimensions between periodontitis patients and healthy individuals were compared, and the distribution of alveolar bone defects was analyzed. A classification system was established regarding the sagittal positions and angulations of the teeth. The buccal residual bone was thicker and the lingual bone was thinner in the periodontitis patients than in the periodontally healthy individuals, and there were differences between the different tooth types, sexes and age subgroups. The buccal undercut was close to the alveolar ridge, while fenestration was reduced and the apical bone height was higher in periodontitis patients than in periodontally healthy individuals. The apical bone height increased with the aggravation of bone loss and age. The proportions of different sagittal positions changed with the aggravation of bone loss. Moreover, the teeth moved more buccally regarding the positions of the maxillary anterior teeth. The morphology of the alveolar bone at the maxillary anterior teeth differed between periodontitis patients and healthy individuals, and the differences were related to the degree of bone loss, tooth type, sex and age.