Objective To investigate the effect and safety of phacoemulsification and intraocular lens implantation in advanced and middle aged senile patients. Methods Ninety five patients (80 96 years old, 100 eyes) with senile cataract were included in advanced aged group. Another 665 patients (60 79 years old, 671 eyes) with senile cataract were included in middle aged group. All patients were performed with phacoemulsification and intraocular lens implantation through a corneal limbus tunnel incision. Results Visual acuity for the advanced aged group at 1 day, one week and one month after operation was 0 5 or better in 30 eyes, 52 eyes and 74 eyes, respectively. Both groups were followed up from 3 to 36 months after operation. The visual acuity of 0 5 or better were followed up in 82 eyes (82 0%) in the advanced aged group and in 583 eyes (86 9%) in the middle aged group. The postoperative complications included tears of posterior capsule, detached vitreous, subluxation of lens, corneal edema, and muddy posterior capsule. Conclusions Phacoemulsification and intraocular lens implantation is effective and safety for both advanced and middle aged patients with senile cataract.

Objective: To analyze the neglect status of children aged 3-6 years old from non-only-child families in Beijing-Tianjin-Hebei region and influencing factors, so as to provide assistance for the healthy growth of preschool children from non-only-child families. Methods: From April to June 2022, 2 882 parents of preschool children from 15 kindergartens were selected in urban areas of Beijing-Tianjin-Hebei by randomized cluster sampling method, and were investigated with general information and neglect experiences. The χ 2 test, t-test and ANOVA were used to compare the neglect status of different categories of preschool children, and the binary Logistic regression method was used to analyze the influencing factors of the neglect of preschool children who were not only children. Results: The detection rate of neglect among children aged 3-6 years from non-only-child families in the Beijing-Tianjin-Hebei region was 33.47%, the detection rate of neglect among children from only-child families was 9.24%, and the difference was statistically significant ( χ 2=261.94, P <0.01). The neglect score for children aged 3-6 years in non-only-child families was (42.35±7.52), the neglect score for onechild families was (38.16±6.56), and the difference was also statistically significant ( t=15.95, P <0.01). Binary Logistic regression analysis showed that family income <4 000 yuan/month ( OR= 2.75, 95%CI =1.18-6.32), grandparents or other relatives as the primary guardian ( OR=2.18, 95%CI =1.35-3.52), and poor parental relationship ( OR=2.64, 95%CI =1.42-3.87), average ( OR=2.31, 95%CI =1.32-4.01) non-only-child preschoolers had a higher risk of neglect ( P <0.05). Conclusion Neglect is prevalent among 3-to 6-year-old children from non-only-child families in the Beijing-Tianjin-Hebei region. Targeted intervention measures based on influencing factors should be taken to reduce neglect among preschool children.

Objective To investigate the clinical value of magnetic resonance cholangiopancreatography (MRCP) in diagnosing biliary tract variability. Methods The accuracy of MRCP was verified by comparing the biliary tract variability found by preoperative MRCP examination with the biliary variability found during surgery. Results The accuracy of biliary tract variability by preoperative MRCP and intraoperative confirmation was 96. 7％. It turned out that the presence of biliary variability could be detected. Conclusion As a noninvasive imaging examination method, MRCP has high accuracy in the diagnosis of biliary tract variability.

FYVE and coiled-coil domain containing 1(FYCO1) is an adaptor of cellular autophagy which has RUN domain, coiled coil domain, FYVE domain, GOLD domain and LIR domain.FYCO1 protein is widely expressed and mainly interacts with Atg8 family proteins, microtubule-based kinesins, phosphatidylinositol-3-phosphate (PI3P). The FYCO1 protein involved in the movement of kinesins along microtubules and the microtubule plus end-directed transport of autophagy vesicles and related to the development and transparency maintenance of human lens.FYCO1 mutations are one of the causes inducing autosomal recessive congenital cataract.Mutations of FYCO1 can inhibit the process of autophagosome transport to lysosomes, leading to the failure of mitochondrial and other organelle degradation processes in lens fibroblasts and causing opacity of the lens.Eighteen cataract-related mutations have been identified in FYCO1 currently.In addition, FYCO1 protein plays an important role in life processes, such as cell division, and is associated with various diseases, such as Parkinson's disease, cancer, sporadic inclusion body myositis and keloid.This article reviewed the current research progress of FYCO1 gene mutations.

Cataract is the leading cause of blindness worldwide, which is a generally clinical and genetic heterogeneity eye disease.To date, more than 50 genes have been reported to be associated with congenital cataract.While for mouse, parts of human cataract related genes knockout mouse can also resulted cataract.As we know, the model of cataract related gene knockout mice can help us to understand the phenotype, pathogenesis, progress and prognosis of human cataract and it can also help to find unknow genes that not reported in human cataract yet.In this review, knockout mouse models with cataract are summarized, which can help to facilitate identification of cataract genes and clarification of the mechanisms of cataractgenisis.