1.Dihydroartemisinin attenuates ischemia/reperfusion-induced renal tubular senescence by activating autophagy.
Huiling LIU ; Zhou HUANG ; Hong JIANG ; Ke SU ; Zilin SI ; Wenhui WU ; Hanyu WANG ; Dongxue LI ; Ninghua TAN ; Zhihao ZHANG
Chinese Journal of Natural Medicines (English Ed.) 2023;21(9):682-693
Acute kidney injury (AKI) is an important factor for the occurrence and development of CKD. The protective effect of dihydroartemisinin on AKI and and reported mechanism have not been reported. In this study, we used two animal models including ischemia-reperfusion and UUO, as well as a high-glucose-stimulated HK-2 cell model, to evaluate the protective effect of dihydroartemisinin on premature senescence of renal tubular epithelial cells in vitro and in vivo. We demonstrated that dihydroartemisinin improved renal aging and renal injury by activating autophagy. In addition, we found that co-treatment with chloroquine, an autophagy inhibitor, abolished the anti-renal aging effect of dihydroartemisinin in vitro. These findings suggested that activation of autophagy/elimination of senescent cell might be a useful strategy to prevent AKI/UUO induced renal tubular senescence and fibrosis.
Animals
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Kidney
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Acute Kidney Injury/chemically induced*
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Ischemia
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Reperfusion Injury/drug therapy*
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Autophagy
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Reperfusion