ABSTRACTOBJECTIVE To discuss the clinical effect of Erdi Biejia decoction together with low dose of Tadalafil in treating diabetic erectile dysfunction.METHODS 70 patients with DED were randomly divided into two groupsthe control group35 cases and the treatment group35 cases.The treatment group was given Erdi Biejia decoction one dosage every dayorally taken in the morning and evening.Tadalafil pills5mg were orally taken once every night for eight weeks.The control group took Tadalafil pills5mg once every night for eight weeks.Before and after the treatmentIIEF?5 scores and nocturnal penile tumescence were recorded.RESULTS After the treatmentIIEF?5 scores and nocturnal penile tumescence of both groups sig-nificantly improvedP <0.01.After the treatmentthe treatment group performed better than the control groupP <0.05. So serious side effects showed in the two groups.CONCLUSION Erdi Biejia decoction together with low dose of Tadalafil were effective in treating DED and were worth of being widely applied in clinical practices.

In order to achieve the purpose of rapid detection of duck astrovirus type 1(DAstV-1),specific primers were designed based on the conservative region of ORF1a which belonged to DAstV-1(WF1202 strain).A real-time fluorescent quantitative PCR(qPCR)detective method for DAstV-1 was established.Clinical samples were detected by the qPCR method and the positive samples were used for virus isolation and identification.Results showed that the detection limit of the established method was 4.64×103 copies/μL,which was 10 times higher than the normal RT-PCR method.In addition,no cross-reactions were found with other common infectious disease pathogens in poultry,indicating that the qPCR method had good specificity.What's more,the coef-ficient of variations(Cv)in intra-and inter-assays were 0.85％-2.85％and 0.21％-2.94％,re-spectively,both less than 3％,indicating that the qPCR method had a good repeatability.Using this method,35 tissue samples from different duck farms in 10 provinces from 2020 to 2022 were detected for DAstV-1.Results showed that the positive rate was 25.71％(9/35),and the coinci-dence rate was 94.29％when compared with the normal RT-PCR method.A positive sample ran-domly taken for the virus isolation through duck embryo passage,and the allantoic fluid was col-lected and then was verified by the qPCR method and inoculated with 1-day-old healthy ducklings for the animal regression experiment.The infected ducklings suffered from transient disease but did not die.The liver tissues were all positive with DAstV-1 when detected by qPCR.Meanwhile,autopsy showed that there were slight changes in the livers,and the histopathological observation showed that the liver cells were steatosis.These findings indicated that the isolated DAstV-1 strain had weak pathogenicity and might be a low virulent strain.To sum up,the qPCR detection method of DAstV-1 was successfully established in this work,and could provide technical support for clini-cal diagnosis,isolation and identification,and molecular epidemiology monitoring of DAstV-1.