ObjectiveTo study the dynamic express of CD57 on T cell of PBMC and clinical significance in acute HIV infection.MethodsSeventeen patients with acute HIV infection were enrolled study randomly diagnosed from 2006.11 to 2009.12 and 15 healthy donors as control group.The PBMCs from 1th,3th and 6th during acute infection were collected.The proportion of CD3+CD57+T lymphocytes,CD3+ CD8+CD57 +T lymphocytes and CD3 + CD4 + CD57 + T lymphocytes were evaluated by flow cytometric analysis with three or double color staining.The relationship between the proportion of CD57+ T phenotypes and virus load and CD4+T cells count was analyzed.ResultsThe proportion of CD57+T lymphocytes in PMBC in 1th,3th and 6th during acute HIV acute was 15.24％ ±1.49％,13.51％ ±2.45％ and 14.65％ ±1.83％,respectively,and was higher than normal control group and the difference was significantly(P＜0.0001 ).The proportion of CD8+ CD57+T lymphocytes was 7.79％ ±2.10％ and 9.88％ ±2.36％ in 1th and 3th month during acute infection,respectively.The proportion of CD8+ CD57+T lymphocytes in 1th and 3th month during acute infection were positive relationship with virus load in corresponding time,and R2 was 0.3700 and 0.3768,and P value was 0.0096 and 0.0088,respectively.The proportion of CD8+CD57+T lymphocytes in the 1th and 3th month during acute infection was negative relationship with CD4+T lymphocytes count.The R2 was 0.3768 and 0.4235,and P value was 0.0215 and 0.0017,respectively.In 6 rapid progressors and 11 no rapid progressors on the 1th month after HIV infection,CD8+ CD57+T lymphocytes percentage was 11.20％±2.21％ and 6.16％ ±1.09％,respectively,and CD4+CD57+T lymphocytes percentage 2.79％ ±0.31％and 1.40％ ±0.30％,respectively.Both CD8+CD57+T and CD4+CD57+T in rapid progressors were higher than no rapid progressors,and P value was 0.0338 and 0.0106,respectively.ConclusionCD57+ T lymphocytes percentage in peripheral blood increase in acute HIV infection patients,in which the increasing CD8+CD57+T lymphocyte may mirror the dynamic of HIV replication and CD4+T cell count.The CD57 high express on T lymphocyte on the early HIV acute infection predicts rapid progression.

Objective To analyze the prevalence,burden and risk factors of mental disorders in Chinese population from 1990 to 2019.Methods Data on disease burden of mental disorders in China from 1990 to 2019 was obtained from the Global Burden of Disease(GBD)in 2019.The prevalence,disability adjusted of life years(DALY)and burden attributable to risk factors of mental disorders were analyzed.Joinpoint was used to estimate the annual percent change average(APC)and the annual percent change(AAPC)of DALY rate of mental disorders and analyze the trend of disease burden.Results In 2019,the prevalence of mental disorders was 11260.42/100000.Among mental disorders,depressive disorder(3519.26/100000)and anxiety disorder(3363.60/100000)had the highest prevalence.In 2019,the DALY rate of females(1564.22/100000)was higher than that of males(1294.51/100000).Depressive disorder(531.65/100000),anxiety disorder(321.60/100000)and schizophrenia(250.99/100000)had the highest DALY rates,accounting for 37.26%,22.54%and 17.59%of the total DALY rates of mental disorders respectively.From 1990 to 2019,the DALY rate of mental disorders in China increased from 1321.15/100000 to 1426.78/100000,the age-standardized DALY rate decreased with an average annual decrease of 0.19%(95%CI:-0.22%～-0.15%),and the age-standardized DALY rate of eating disorders increased the most(AAPC=1.94%,95%CI:1.85%～2.02%).The DALY rate of depressive disorder attributable to intimate partner violence was the highest in China in 2019(26.72/100000).The DALY rate of male depression attributed to childhood sexual abuse increased significantly from 1990 to 2019(AAPC=0.68%,95%CI:0.26%～1.09%).Conclusion Attention should be paid to the prevention and treatment of mental disorders,screening and intervention measures should be carried out among high risk populations,and etiological prevention should be carried out according to risk factors to reduce the burden of mental disorders.

Objective: To understand the current situation and differences in physical activity among children and adolescents in different regions of China, so as to provide a scientific reference for promoting the level of overall physical activity promotion among children and adolescents in China. Methods: From June to December 2023, the student satisfaction assessment questionnaire for the national children and adolescents theme fitness activity "Run, Youth!" was conducted, which was administered via a combined online and offline questionnaire survey to explore 397 013 students participation in the "Run, Youth!" activity in 2023. Descriptive statistics, Logistic regression, and other approaches were utilized for data analysis. Results: There were notable disparities in the rates of children and adolescents meeting the physical activity standards among northeast China, northwest China, north China, east China, central China and southwest China (10.27%, 11.79%, 9.32%, 8.48%, 8.81%, 7.30%) ( χ 2=33.48, P <0.05). The results of multivariate Logistic regression analysis showed that rate of recommended level of physical activity among children in northwest China, boys, children aged 16-18, children in urban areas were relatively high ( OR =1.12, 1.68, 0.60, 1.88, P < 0.05 ). After adjusting for confounding factors, the results of binary Logistic regression analysis showed that there was an interactive effect relationship between the interaction of region, age, gender, and urban-rural distribution and physical activity among children and adolescents ( OR=0.51, P <0.01). Based on gender analysis, weekly physical activity duration of boys in each region surpassed that of girls; based on age analysis, except for the central and southwestern regions, weekly physical activity duration of children and adolescents gradually increased with age in other regions; based on the proportion of urban and rural areas, weekly physical activity duration of children and adolescents in urban areas across all regions exceeded that in rural areas. Conclusions The attainment rate of sufficient physical activity among children and adolescents in northwest China is the highest, and the differences between regions are not significant. The participation and level of physical activity of male students in each region surpass those of females and the attainment rate of physical activity of physical activity of urban children and adolescents is higher than that of those in rural areas.