2.Functional magnetic stimulation can improve the urination of persons with urine retention after a spinal cord injury
Chen SONG ; Jiang LI ; Chao HAN ; Jie HAN ; Chuanmei YANG ; Zihan XU
Chinese Journal of Physical Medicine and Rehabilitation 2022;44(1):57-61
Objective:To observe any effect of functional magnetic stimulation (FMS) of the sacral 3 nerve root on relieving urine retention after a spinal cord injury.Methods:Forty-four patients with neurogenic bladder disorders after a spinal cord injury were divided at random into a 5Hz group ( n=14), a 20Hz group ( n=15), and a sham stimulation group ( n=15). In addition to conventional bladder control training, the 5Hz and 20Hz groups were given 20 minutes of FMS of the sacral 3 nerve root at the appropriate frequency five days a week for 4 weeks. The sham stimulation group was not given any stimulation. A detailed urination diary was kept during the treatment, and before and after the 4 weeks, everyone′s bladder pressure volume was assessed and an electromyogram was recorded. Results:After the treatment those in the 5Hz and 20Hz groups had improved significantly in terms of average bladder capacity, bladder pressure, residual urine volume, daily number of urethral catheterizations, daily micturition frequency, single urine output, H-reflex latency, and the amplitude and incubation period of the F wave. Those in the sham stimulation group showed no consistent improvement in any of these indicators. Moreover, the average residual urine volume, daily urination frequency and H-reflex latency and amplitude of the 20Hz group were significantly better than those of the 5Hz group.Conclusions:Magnetic stimulation of the S3 nerve roots can effectively improve the urination of persons with neurogenic bladder disorders after a spinal cord injury. The preferred frequency is 20Hz.
3.Reliability of implant stability measuring devices depending on various clinical conditions: an in vitro study
Han-Na LEE ; Myoung-Sub KIM ; Jeong-Yol LEE ; Xu ZIHAN ; Jae-Jun RYU ; Ji-Suk SHIM
The Journal of Advanced Prosthodontics 2023;15(3):126-135
PURPOSE:
. The aim of this study was to evaluate the reliability of implant stability measuring devices depending on the location of the implant and the position of the patient.
MATERIALS AND METHODS:
. Six implants were installed in different dentate sextants of six artificial bone models. Implant stability was measured in three conditions of the bone model (without mounting on a phantom head, mounted on a phantom head in supine position, and mounted on a phantom head in upright position). A resonance frequency analysis device (Osstell) and two damping capacity analysis devices (Periotest and Anycheck) were used to measure implant stability. The values measured outside the phantom head were treated as controls, and the values inside the phantom head were compared using an independent t-test.
RESULTS:
. Osstell showed different results in two of the six divisions in both the supine and upright positions compared to outside of the mouth (P < .05). Periotest showed different results in all six parts in the supine position and in five parts in the upright position compared to outside of the mouth (P < .05). While Anycheck showed different results in five areas in the supine position compared to outside of the mouth, it showed different results in only one area in the upright position (P < .05).
CONCLUSION
. In the difficult implant position for the operator to access, the implant stability measuring devices show less reliability. The accessibility of implant is greatly affected in the order of Osstell, Anycheck, and Periotest. [J Adv Prosthodont 2023;15:126-35]
4.Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses
Zihan ZHAO ; Yue LIU ; Yushi ZHANG ; Zeyu GENG ; Rina SU ; Lipeng ZHOU ; Chao HAN ; Zhanjun WANG ; Shuangcheng MA ; Weidong LI
Journal of Pharmaceutical Analysis 2022;12(5):733-742
Pruni Semen,the seed of several unique Prunus plants,is a traditional purgative herbal material.To determine the authentic sources of Pruni Semen,46 samples from four species were collected and analyzed.Ten compounds including multiflorin A(Mul A),a notable purative compound,were isolated and identified by chemical separation and nuclear magnetic resonance spectroscopy.Seventy-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry,and acetyl flavonoid glycosides were recognized as characteristic constituents.The flavonoids were distributed in the seed coat and cyanogenic glycosides in the kernel.Based on this,methods for identifying Pruni Semen from different sources were established using chemical fingerprinting,quantitative analysis of the eight principal compounds,hierarchical cluster analysis,principal component analysis,and orthogonal partial least squares discriminant analysis.The results showed that the samples were divided into two categories:one is the small seeds from Prunus humilis(Ph)and Prunus japonica(Pj),and the other is the big seeds from Prunus pedunculata(Pp)and Prunus triloba(Pt).The average content of Mul A was 3.02.6.93,0.40,and 0.29 mg/g,while the average content of amygdalin was 18.5,17.7,31.5,and 30.9 mg/g in Ph,Pj,Pp,and Pt,respectively.All the above information suggests that small seeds might be superior sources of Pruni Semen.This is the first comprehensive report on the identification of chemical components in Pruni Semen from different species.
5.Application and progress of intraoperative neuromonitoring techniques in robotic thyroid surgery
Cheng WANG ; Chengqiu SUI ; Zihan ZHAO ; Han ZHANG ; Daqi ZHANG ; Hui SUN
Chinese Journal of Endocrine Surgery 2022;16(1):126-128
Robotic surgical systems are gradually being used in minimally invasive surgery with their advantages of high-definition magnified 3D images, stable surgical field and flexible operation. The change of surgical approach and the narrow operating space in robotic thyroid surgery have made it more difficult to identify and protect the laryngeal nerve, and the application of nerve monitoring has been limited. Many researchers have attempted to improve the monitoring equipment and probe placement to make intraoperative neuromonitoring techniques work well in robotic thyroid surgery. In this paper, we seek effective ways to protect the laryngeal nerve in robotic thyroid surgery, and lay the foundation for a more minimally invasive and standardized development of this technology.
6.Efficacy and safety of glucokinase activators for type 2 diabetes mellitus therapy:a meta-analysis
Jie LI ; Xiaofei CHEN ; Zihan XU ; Xiaoyan LIU ; Yuechan ZHANG ; Yi HAN ; Wei ZHANG ; Zhitao JIANG
China Pharmacy 2023;34(1):102-106
OBJECTIVE To systematically evaluate the efficacy and safety of glucokinase activators in the treatment of type 2 diabetes mellitus. METHODS PubMed, Cochrane Library, Web of Science, Embase and CNKI databases were searched from the inception to March 2022. Randomized controlled trials about glucokinase activators versus placebo (or other oral hypoglycemic agents) in the treatment of type 2 diabetes were included, data were extracted and meta-analysis was analyzed using RevMan 5.4 software. RESULTS A total of 9 studies with 215 0 patients were included. In terms of hypoglycemic effect, compared with control group, glucokinase activators significantly reduced glycosylated hemoglobin (HbA1c) [MD=-0.40, 95%CI(-0.53, -0.26), P<0.000 01], fasting blood glucose[MD=-0.53, 95%CI(-0.85, -0.20), P=0.001] and 2 h postprandial blood glucose [MD=-2.28, 95%CI(-2.68, -1.88), P<0.000 01] in diabetic patients. In terms of safety, the incidence of hypoglycemia caused by glucokinase activators was higher than control group on the whole [RR=1.55, 95%CI(1.20,2.01), P= 0.000 8]. According to the subgroup analysis of organs activated by glucokinase activator, the incidence of hypoglycemia in the pancreas-liver dual activator group [RR=1.44, 95%CI(1.11,1.89), P=0.007] and liver-selective activator group [RR=2.26, 95%CI(1.02,5.03), P=0.05] was higher than that in the control group, the difference was statistically significant. CONCLUSIONS Glucokinase activators can effectively reduce HbA1c, fasting blood glucose and 2 h postprandial blood glucose in patients with type 2 diabetes, but the risk of hypoglycemia remains to be addressed.
7.Alteration of gut microbiota in type 2 diabetes complicated with cholelithiasis patients.
Jiajia CHEN ; Linlin YAN ; Xingfan MA ; Ping YUAN ; Fan ZHAO ; Zihan HAN ; Jingshan LIU ; Wenbo WANG ; Donghai ZHOU ; Hongyu ZHAO ; Nan FENG ; Dandan HUANG ; Shoukui HU ; Jin GU
Chinese Medical Journal 2022;135(17):2125-2127
8. Clinicopathological characteristics of type 2 diabetes mellitus complicated with colorectal cancer
Zihan HAN ; Jiajia CHEN ; Nan FENG ; Pengfei NIU ; Can SONG ; Zhaoya GAO ; Qingkun GAO ; Dengbo JI ; Wenbo WANG ; Fuming LEI ; Jin GU
Chinese Journal of Gastrointestinal Surgery 2019;22(10):966-971
Objective:
To analyze the clinicopathological features of type 2 diabetes mellitus complicated with colorectal cancer (DCRC).
Methods:
A case-control study was conducted. Inclusion criteria: (1) hospitalized patients receiving fibrocolonoscopy; (2) adenocarcinoma and mucinous adenocarcinoma diagnosed by pathology; (3) with preoperative cTNM clinical staging; (4) colorectal cancer patients undergoing surgical treatment; (5) with postoperative pTNM staging; (6) no smoking or drinking habits. Exclusion criteria: (1) familial adenomatous polyposis (FAP); (2) Lynch syndrome; (3) carcinoma of anal canal and perianal carcinoma; (4) multiple primary cancer; (5) with serious cardiocerebrovascular diseases or multiple organ failure. Clinicopathlogical data of 32 DCRC patients who were diagnosed and treated in Peking University Shougang Hospital from December 2017 to December 2018 were retrospectively collected and analyzed. Forty nondiabetic colorectal cancer (CRC) patients during the same period were selected as control group according to the sex ratio and the age difference less than 5 years. Student′s
9.Burden of vitiligo on Chinese patients: An online survey.
Abdulrahman AMER ; Yan WU ; Chunying LI ; Juan DU ; Hong JIA ; Shanshan LI ; Caixia TU ; Qiang LI ; Hongxia LIU ; Junling ZHANG ; Tao LU ; Jinsong LIU ; Aihua MEI ; Han LIU ; Fei TIAN ; Chong LU ; Zihan LI ; Lixin CAO ; Xinghua GAO
Chinese Medical Journal 2023;136(19):2365-2367
10.Metagenomic and targeted metabolomic analyses reveal distinct phenotypes of the gut microbiota in patients with colorectal cancer and type 2 diabetes mellitus.
Yong YANG ; Zihan HAN ; Zhaoya GAO ; Jiajia CHEN ; Can SONG ; Jingxuan XU ; Hanyang WANG ; An HUANG ; Jingyi SHI ; Jin GU
Chinese Medical Journal 2023;136(23):2847-2856
BACKGROUND:
Type 2 diabetes mellitus (T2DM) is an independent risk factor for colorectal cancer (CRC), and the patients with CRC and T2DM have worse survival. The human gut microbiota (GM) is linked to the development of CRC and T2DM, respectively. However, the GM characteristics in patients with CRC and T2DM remain unclear.
METHODS:
We performed fecal metagenomic and targeted metabolomics studies on 36 samples from CRC patients with T2DM (DCRC group, n = 12), CRC patients without diabetes (CRC group, n = 12), and healthy controls (Health group, n = 12). We analyzed the fecal microbiomes, characterized the composition and function based on the metagenomics of DCRC patients, and detected the short-chain fatty acids (SCFAs) and bile acids (BAs) levels in all fecal samples. Finally, we performed a correlation analysis of the differential bacteria and metabolites between different groups.
RESULTS:
Compared with the CRC group, LefSe analysis showed that there is a specific GM community in DCRC group, including an increased abundance of Eggerthella , Hungatella , Peptostreptococcus , and Parvimonas , and decreased Butyricicoccus , Lactobacillus , and Paraprevotella . The metabolomics analysis results revealed that the butyric acid level was lower but the deoxycholic acid and 12-keto-lithocholic acid levels were higher in the DCRC group than other groups ( P < 0.05). The correlation analysis showed that the dominant bacterial abundance in the DCRC group ( Parvimonas , Desulfurispora , Sebaldella , and Veillonellales , among others) was negatively correlated with butyric acid, hyodeoxycholic acid, ursodeoxycholic acid, glycochenodeoxycholic acid, chenodeoxycholic acid, cholic acid and glycocholate. However, the abundance of mostly inferior bacteria was positively correlated with these metabolic acid levels, including Faecalibacterium , Thermococci , and Cellulophaga .
CONCLUSIONS
Unique fecal microbiome signatures exist in CRC patients with T2DM compared to those with non-diabetic CRC. Alterations in GM composition and SCFAs and secondary BAs levels may promote CRC development.
Humans
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Gastrointestinal Microbiome/genetics*
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Diabetes Mellitus, Type 2
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Microbiota
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Bacteria/genetics*
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Fatty Acids, Volatile
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Colorectal Neoplasms/metabolism*
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Butyrates
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Feces/microbiology*