1.Artemisinins regulate apoptosis genes of neoplasm
International Journal of Traditional Chinese Medicine 2008;30(2):119-121
Artemisinin and its derivatives,which are known as traditional antimalarial drugs,have anticancer activities by inhibiting cell proliferation,inducing apoptosis of tumor cells,and resisting angiogenesis,etc.The activity of inducing apoptosis has become high-light for studies recently.Most scholars believed this activity was obtained by increasing apoptosis-inducing genes(Bax),decreasing anti-apoptosis genes(Survivin,Bcl-2),and regulating two-ways controlling genes,and finally led to the distinctive morphological changes.
2.Biology effect research of dihydroartemisinin induce the apoptosis in PC-3 cell lines of prostate cancer enhanced by low frequency ultrasound
Jing WANG ; Ziguo LUO ; Jian LI
Chongqing Medicine 2013;(23):2706-2709
Objective To study the enhancement effects of the low frequency ultrasound to the apoptosis induced by dihydroar-temisinin in the transplantation human prostate cancer PC-3 cells in nude mice ,and to explore the underlying action mechanism . Methods Prostate cancer PC-3 cells were transplanted into 20 nude mice to establish the solid tumor mode .These nude mice were randomly divided into 4 groups with 5 mice in each group :control group ,dihydroartemisinin group ,ultrasound group and ultrasound+ dihydroartemisinin group .After 13 days and 7 times drug administration ,The positive products of Bcl-2 ,Bax ,VEGF ,Caspase-3 and CHOP were tested by immunohistochemical method .Results The tumor volume were larger in control group and ultrasound group through observed by tumor contour ,and there were liquefication and necrosis region after incised the tumor tissue .Immuno-histochemical examination revealed that the positive products of Bcl-2 and VEGF were decreased in ultrasound+DHA group ,DHA group and ultrasound group .Immunohistochemical examination revealed that the positive products of Bax 、Caspase-3 and CHOP were significantly increased in ultrasound+DHA group ,DHA group and ultrasound group .Conclusion The low frequency ultra-sound can′t cause obvious damage to the normal cell ,which could causing the change of the positive products in transplantation hu-man prostate cancer PC-3 cells .The results show that DHA has this action alone ,the ultrasound may enhance the apoptosis of PC-3 cells induced by DHA.
3.Analysis and verification of gastric cancer metastatic lymphonodus associated long non-coding RNA
Ziguo YANG ; Xiaobo GUO ; Yulong SHI ; Changqing JING ; Leping LI
Chinese Journal of General Surgery 2015;30(10):800-803
Objective To analyze and verify the expression profiles of long non-coding RNAs (lncRNAs) in gastric cancer (GC) metastatic lymphonodus.Methods Microarray analysis was performed in 3 GC positive lymphonodus and 1 normal lymph node with Agilent Array platform to measure the expression levels of lncRNAs and mRNAs and to investigate the expression differences of lncRNAs in GC metastatic lymphonodus and normal lymphonodus, and hierarchical clustering used to screen out the differently expressed lncRNAs.15 up-regulated lncRNAs and 15 down-regulated lncRNAs were randomly chosen and RT-PCR was used to verify the expression differences.Results Comparing with normal lymphonodus, 353 lncRNAs and 547 mRNAs are up-regulated, but 464 lncRNAs and 562 mRNAs are down-regulated in GC metastatic lymphanodus as 6 times or more variation was found.The expressions of lncRNA OR3A4, LOC84740, FCGR1C and C21orf 96 were increased in GC metastatic lymphonodus, but lncRNA MSTO2P, LOC344595, TUG1, TYW3 and KRT8P10 decreased.Conclusions LncRNAs are aberrantly expressed in GC metastatic lymphonodus.
4.Induction of dihydroartemisinin on prostate cancer PC-3 apoptosis and its mechanism
Xiaoling GAO ; Ziguo LUO ; Pilong WANG ; Qingchun LI
Chinese Traditional and Herbal Drugs 1994;0(01):-
Objective To study the induction of dihydroartemisinin(DHA) on prostate cancer PC-3 apoptosis and its possible mechansim.Methods MTT was employed for cellular viability measurement,flow cytometry(FCM) and transmission electron microscopy(TEM) for observation of apoptosis,and immunocytochemical staining(SP) for analyzing the expression of Bcl-2 and Bax proteins in PC-3 cells treated with DHA of different concentration.Results DHA Significantly inhibited the proliferation of PC-3 cells,induced their apotosis in a time-concentration dependent manner,and led to mitochondrial swelling,nuclear fragmentations and apoptosis body formation,down-expression of Bcl-2 protein,and over-expression of Bax protein correspondence with DHA concentration.Conclusion DHA could induce the apoptosis in PC-3 cells by up-regulating Bax protein and down-regulating Bcl-2 protein.
5.Clinical observation of antimicrobial dressings tamponade combined with seal negative pressure drainage in the treatment of 52 cases of necrotizing fasciitis
Yang LI ; Xiaodong GUO ; Ziguo LI ; Huizhuan CUI ; Aiguo XU ; Hui WANG
Chinese Journal of Primary Medicine and Pharmacy 2018;25(5):595-597
Objective To observe the effect of antibacterial dressing tamponade combined with seal negative pressure drainage in the treatment of necrotizing fasciitis .Methods 52 patients with necrotizing fasciitis were selected . After full scavenging of fasciitis and necrotic tissue,then filled the whole wound with a kind of dressings with antibacterial properties-Nano silver antiseptic dressing,and a negative pressure drainage tube was placed at the bottom of the wound.Then the wound was closed with a transparent paste ,and the air was cut off,in order to make the antibacterial dressing could not only anti bacteria ,but also play a role in the growth of the wound ,and at the same time,the tissue leachate in the wound was drained through the negative pressure tube in time ,so as to accelerate the wound healing and reduce the use of antibiotics .Results Among the 52 cases,the rest were cured except for 1 case of individual cause of abandonment of treatment died of toxic shock .,the average hospitalization time was (29.0 ±15.3)days,days of using antimicrobial was (3.8 ±1.6)days,90% cases stopped using antibiotics within 1 week.Conclusion Antibacterial dressing tamponade combined with seal negative pressure drainage in the treatment of necrotizing fasciitis can reduce the switching frequency,reduced antibiotic using intensity,shorten the healing time,it is a kind of very good method.
6.The impact of night work near the day of ovulation on outcome of pregnancy.
Peizhi LI ; Ziguo FANG ; Xiaochuan PAN ; Lihua WANG ; Xiping XU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(5):369-371
OBJECTIVETo investigate the effect of night work near the day of ovulation on outcome of pregnancy.
METHODSDaily early morning urine samples were collected from eligible shift women workers, and human chorionic gonadotropin(hCG), follicle-stimulating hormone(FSH), the urinary steroid metabolites of estrogen and progesterone(PDG, E1C) were measured to confirm the day of ovulation and early fetal loss(EFL). Questionnaires were used to know workshift, other occupation exposures, and related information.
RESULTS12 clinical spontaneous abortion(SAB), 18(EFL) and 44 clinical living birth were analyzed in relation to SAB, EFL and night shift near the day of ovulation by using single factor and multi-factor logistic regression. The result showed that night shift on the day of ovulation and the day before or after ovulation was related with SAB both in single- and muti-factorial analysis(single factor: OR = 2.48, 95% CI 1.10-5.60; multi-factor; OR = 3.90, 95% CI 1.28-11.90).
CONCLUSIONNight shift near the day of ovulation may be related to SAB.
Abortion, Spontaneous ; etiology ; Chorionic Gonadotropin ; blood ; Female ; Fetal Death ; etiology ; Follicle Stimulating Hormone ; blood ; Humans ; Logistic Models ; Ovulation ; Pregnancy ; Work Schedule Tolerance
7.Progress of the pathogenesis in Merkel cell carcinoma
Shaojun XUE ; Mengyuan ZHANG ; Ziguo WANG ; Juntao ZHOU ; Xianfeng LI
Cancer Research and Clinic 2022;34(9):710-713
Merkel cell carcinoma (MCC) is a rare primary neuroendocrine carcinoma of the skin, which is named for its ultrastructure and immunophenotype similar to Merkel cells in the skin. It has been found that the integration of MCC with the oncogenic Merkel cell polyomavirus (MCPyV) may drive tumorigenesis or cause somatic mutations to the development of MCC because of ultraviolet ray-induced DNA damage. However, the pathogenesis of MCC is still unclear. This article introduces the current research progress of the pathogenesis of MCC, hoping to provide theoretical guidance for follow-up researches.
8. A multicenter retrospective study on clinical value of lymph node dissection in the radical resection of intrahepatic cholangiocarcinoma
Lei WANG ; Ziguo LIN ; Tian YANG ; Jianying LOU ; Shuguo ZHENG ; Xinyu BI ; Jianming WANG ; Wei GUO ; Fuyu LI ; Jian WANG ; Yamin ZHENG ; Jingdong LI ; Shi CHENG ; Yongyi ZENG ; Jingfeng LIU
Chinese Journal of Digestive Surgery 2020;19(1):72-80
Objective:
To investigate the clinical value of lymph node dissection (LND) in the radical resection of intrahepatic cholangiocarcinoma (ICC).
Methods:
The propensity score matching and retrospective cohort study was conducted. The clinicopathological data of 448 patients with ICC who were admitted to 12 medical centers from December 2011 to December 2017 were collected, including 279 in the Eastern Hepatobiliary Surgery Hospital of Navy Medical University, 32 in the Mengchao Hepatobiliary Hospital of Fujian Medical University, 21 in the First Hospital Affiliated to Army Medical University, 20 in the Cancer Hospital Chinese Academy of Medical Science and Peking Union Medical College, 19 in the West China Hospital of Sichuan University, 18 in the Second Hospital Affiliated to Zhejiang University School of Medicine, 18 in the Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 16 in the Beijing Friendship Hospital Affiliated to Capital Medical University, 10 in the Xuanwu Hospital Affiliated to Capital Medical University, 7 in the Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, 5 in the Beijing Tiantan Hospital Affiliated to Capital Medical University, and 3 in the Affiliated Hospital of North Sichuan Medical College. There were 281 males and 167 females, aged from 22 to 80 years, with a median age of 57 years. Of the 448 patients, 143 with routinely intraoperative LND were divided into LND group and 305 without routinely intraoperative LND were divided into control group, respectively. Observation indicators: (1) the propensity score matching conditions and comparison of general data between the two groups after matching; (2) intraoperative and postoperative situations; (3) follow-up; (4) survival analysis. Patients were followed up by outpatient examination, telephone interview and email to detect survival of patients and tumor recurrence up to October 31, 2018 or death. Follow-up was conducted once every 3 months within postoperative 1-2 years, once every 6 months within postoperative 2-5 years, and once a year after 5 years. The propensity score matching was realized using the nearest neighbor method with 1∶1 ratio. Measurement data with normal distribution were represented as