Objective:To establish an analytical method for measuring the concentration of glucose in saliva by ion chromatography.Methods:The proteins in saliva were removed by thermal denaturation method, CarboPac PA20 (3×30 mm) was used as a protective column and CarboPac PA20 (3×150 mm) was used as an analytical column for ion chromatography analysis. Gradient elution was carried out with A: ultra-pure water, B: 250 mmol/L NaOH solution and C: 500 mmol/L NaAc solution. Pulsed ampere detector was used for detection.Results:This method had a good linear relationship in the range of 0.04 to 0.12 mg/L, with a linear relation coefficient of 0.9967. The detection limit of glucose was 2 μg/L, the mean value of the relative standard deviation (RSD) of the repeatability measurement was 0.75%, and the average spike recovery was 103.07%.Conclusion:This method is simple, sensitive, accurate and stable, and can be used for the determination of glucose concentration in saliva.

Objective To study the role of call stimulate in arousal of coma patients after severe craniocerebral injury.Methods Twenty patients with severe craniocerebral trauma coma,admitted to our hospital from June 2014 to June 2015,were randomly divided into stimulation group (n=13) and control group (n=7).In addition to the regular relatives call stimulation,other treatments in stimulation group were the same as the control group.One,2,and 4 weeks after call stimulation,Glasgow Coma Scale (GCS) scores,blood flow velocity changes of internal carotid artery (ICA) and middle cerebral artery (MCA),and neurotransmitter contents of5-hydroxytryptamine (5-HT),dopamine (DA),norepinephrine (NE) and acetylcholine (Ach) in the cerebrospinal fluid (CSF) were detected.Results The consciousness was significantly improved in the stimulation group since the second week;at the 4th week,the GCS scores (11.85±2.58) were significantly higher than those in the control group (9.14±2.85,P＜0.05).ICA and MCA blood flow velocity of both groups reached peak level in the first week,and then,gradually decreased till the end of the observation;ICA velocity value in the stimulation group (72.85± 7.45) was lower than that in the control group (82.14±11.61).The DA,Ach and NE content in the stimulation group were significantly increased at the 4th week as compared with those in the control group (P＜0.05);but no significant difference was noted between the two groups in the content of 5-TH (P＞0.05).Conclusion The call stimulus has certain awaking effect on coma patients,whose mechanism may be related to call induced changes of cerebral blood flow velocity and related neurotransmitter release.